Cloning and sequence analysis of cDNA encoding the precursor of a human endothelium‐derived vasoconstrictor peptide, endothelin: Identity of human and porcine endothelin

Abstract

A cDNA encoding a human endothelium-derived vasoconstrictor peptide, endothelin, was isolated from a human placenta cDNA library. The nucleotide sequence of this cDNA clone showed that the primary structure of the human preproendothelin has 212 amino acid residues and is highly homologous to porcine preproendothelin, and that human endothelin is identical with porcine endothelin.

Detection of immunoreactive endothelin in plasma of hemodialysis patients

“…The fact that the dilution curve of large IR-ET paralleled the standard curve suggests that large IR-ET is a precursor of ET. Large IR-ET is probably on intermediate produced from preproendothelin [2] through unknown processing. The findings that the dilution curves of plasma from HD patients were not parallel with the standard curve may indicate the existence of unknown substances in plasma of HD patients which accelerate radioligand binding in the RIA system.…”

“…ET is known to elevate the arterial blood pressure of rats and it has been suggested that disturbances in the control of ET production contribute to the pathogenesis of hypertension [1,2]. However, it remains unknown as to whether ET exists in human plasma.…”

Ultraviolet B irradiation increases endothelin‐1 and endothelin receptor expression in cultured human keratinocytes

“…3A. The PCR products were found to coincide to human ET-1 and ET A receptor, respectively, by direct sequencing [2,29]. mRNA expression.…”

“…ET-1 primers were designed as 5'-TGC TCC TGC TCT TCC CTG ATG GAT AAA GAG TGT GTC-3" (sense) and 5'-GGT CAC ATA ACG CTC TCT GGA GGG CTT-3' (antisense) [27]. The primers were predicted to amplify a 462 base pair DNA fragment (nucleotides 157 to 618) [2]. ET A receptor primers were designed as 5'-AGC TTC CTG GTT ACC ACT CAT CAA-3' (sense) and 5'-T CAA CAT CTC ACA AGT CAT GAG-3' (antisense) [28].…”

“…ET consists of three isopeptides, termed ET-1, ET-2 and ET-3 (ETs), while their receptors are classified, both pharmacologically and molecularly, into ET A and ETB subtypes [2][3][4][5]. The ET A receptor has different affinities for the three ET isoforms in the order of ET-I>ET-2>>ET-3.…”