Cloning and sequence analysis of two embryonic β-like globin cDNAs (y and z) of hamster

Subar, Milayna; Lee, Kai Mon; Boussios, Thalia

doi:10.1016/0167-4781(92)90532-5

Cited by 5 publications

(1 citation statement)

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“…AF314660 and AF314661), revealing 88% identity to the previously cloned (Lee et al, 1992) golden hamster ␤-major globin chain cDNA. In addition, oligonucleotide primers corresponding to the golden hamster embryonic z gene (Li et al, 1992) were used to amplify a fragment from Chinese hamster genomic DNA that was then cloned (pHEB) and used to identify four overlapping phage (JHC2, JHC4, JHC6, and JHC8). A 400-bp PvuII/SacI PCR fragment from JHC2 was then sequenced (EMBL/GenBank/DDBJ under accession nos.…”

Section: Probes and Fishmentioning

confidence: 99%

The replication timing program of the Chinese hamster β-globin locus is established coincident with its repositioning near peripheral heterochromatin in early G1 phase

Feng

Chen

Izumi

et al. 2001

The Journal of Cell Biology

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We have examined the dynamics of nuclear repositioning and the establishment of a replication timing program for the actively transcribed dihydrofolate reductase (DHFR) locus and the silent β-globin gene locus in Chinese hamster ovary cells. The DHFR locus was internally localized and replicated early, whereas the β-globin locus was localized adjacent to the nuclear periphery and replicated during the middle of S phase, coincident with replication of peripheral heterochromatin. Nuclei were prepared from cells synchronized at various times during early G1 phase and stimulated to enter S phase by introduction into Xenopus egg extracts, and the timing of DHFR and β-globin replication was evaluated in vitro. With nuclei isolated 1 h after mitosis, neither locus was preferentially replicated before the other. However, with nuclei isolated 2 or 3 h after mitosis, there was a strong preference for replication of DHFR before β-globin. Measurements of the distance of DHFR and β-globin to the nuclear periphery revealed that the repositioning of the β-globin locus adjacent to peripheral heterochromatin also took place between 1 and 2 h after mitosis. These results suggest that the CHO β-globin locus acquires the replication timing program of peripheral heterochromatin upon association with the peripheral subnuclear compartment during early G1 phase.

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Section: Probes and Fishmentioning

confidence: 99%