Cloning and sequencing an unknown gene of Tropheryma whipplei and development of two LightCycler® PCR assays

Maibach, Romana C.; Altwegg, Martin

doi:10.1016/s0732-8893(03)00063-4

Cited by 18 publications

(5 citation statements)

References 19 publications

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“…In this study, only the 16S and 23S rDNA sequences of the Tropheryma variant were determined. BLAST analysis of 16S rDNA primers described in literature for detection of T. whipplei [12,13] indicates that not all of them will detect the new variant. For example, primer W3AF [14] has a mismatch at its 3 0 end which might interfere with PCR amplification.…”

Section: Discussionmentioning

confidence: 99%

Novel Tropheryma species in a lung biopsy sample from a kidney transplant recipient

Vankeerberghen

Jonckheere

Raeve

et al. 2018

Clinical Microbiology and Infection

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Section: Discussionmentioning

confidence: 99%

Novel Tropheryma species in a lung biopsy sample from a kidney transplant recipient

Vankeerberghen

Jonckheere

Raeve

et al. 2018

Clinical Microbiology and Infection

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“…Thus, qPCR is used to quantify DNA o cDNA, determining gene or transcript numbers present within different samples [25,26,27]. qPCR offers advantages such as speed in the result, the reduced risk of contamination and the ease in handling technology [28,29]. This PCR uses fluorescence detection systems which are generally of two types: intercalating agents and labeled probes with fluorophores.…”

Section: Real Time Pcrmentioning

confidence: 99%

Polymerase Chain Reaction: Types, Utilities and Limitations

Hernández-Rodríguez¹,

Ramírez²

2012

Polymerase Chain Reaction

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“…The PCR technique described by Meibach et al . in 2003 was used [11]. Having diagnosed Tropheryma whipplei right heart endocarditis, we switched the antibiotic regimen to ceftriaxone 2 g once daily.…”

Section: Case Presentationmentioning

confidence: 99%

Tropheryma whipplei tricuspid endocarditis: a case report and review of the literature

et al. 2010

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IntroductionThe main clinical manifestations of Whipple's disease are weight loss, arthropathy, diarrhea and abdominal pain. Cardiac involvement is frequently described. However, endocarditis is rare and is not usually the initial presentation of the disease. To the best of our knowledge, this is the first reported case of a patient with Tropheryma whipplei tricuspid endocarditis without any other valve involved and not presenting signs of arthralgia and abdominal involvement.Case presentationWe report a case of a 50-year-old Caucasian man with tricuspid endocarditis caused by Tropheryma whipplei, showing signs of severe shock and an absence of other more classic clinical signs of Whipple's disease, such as arthralgia, abdominal pain and diarrhea. Tropheryma whipplei was documented by polymerase chain reaction of the blood and pleural fluid. The infection was treated with a combined treatment of doxycycline, hydroxychloroquine and sulfamethoxazole-trimethoprim for one year.ConclusionTropheryma whipplei infectious endocarditis should always be considered when facing a blood-culture negative endocarditis particularly in right-sided valves. Although not standardized yet, treatment of Tropheryma whipplei endocarditis should probably include a bactericidal antibiotic (such as doxycycline) and should be given over a prolonged period of time (a minimum of one year).

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Cloning and sequencing an unknown gene of Tropheryma whipplei and development of two LightCycler® PCR assays

Cited by 18 publications

References 19 publications

Novel Tropheryma species in a lung biopsy sample from a kidney transplant recipient

Novel Tropheryma species in a lung biopsy sample from a kidney transplant recipient

Polymerase Chain Reaction: Types, Utilities and Limitations

Tropheryma whipplei tricuspid endocarditis: a case report and review of the literature

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