Cloning, characterization and computational analysis of the 5′ regulatory region of ovine glucose 6-phosphate dehydrogenase gene

Laliotis, George P.; Bizelis, Iosif; Argyrokastritis, Alexandros; Rogdakis, E.

doi:10.1016/j.cbpb.2007.04.001

Cited by 14 publications

(11 citation statements)

References 36 publications

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“…Combined, these findings suggest that the mRNA expression level of PNPLA3 and G6PD is tightly controlled by the same upstream regulator. The lipogenic transcription factor sterol regulatory element binding protein 1 (SREBP-1) has been identified as a possible regulator of G6PD expression in liver [24,28]. It will therefore be interesting to study whether SREBP can also act as a transcriptional regulator of hepatic PNPLA3 expression.…”

Section: Discussionmentioning

confidence: 98%

The expression level of non-alcoholic fatty liver disease-related gene PNPLA3 in hepatocytes is highly influenced by hepatic lipid status

Hoekstra

Kruijt

et al. 2010

Journal of Hepatology

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Section: Discussionmentioning

confidence: 98%

The expression level of non-alcoholic fatty liver disease-related gene PNPLA3 in hepatocytes is highly influenced by hepatic lipid status

Hoekstra

Kruijt

et al. 2010

Journal of Hepatology

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“…Non-RARE regions located between proximal and distal RAREs, which contain several SP1 and SP3 sites appear to play an important role in the basal promoter activity in HEK293T cells only, but in response to at-RA in both cell types. These transcriptional factors have been shown to interact with RARs in driving the expression of several genes in response to RA (Wolf et al, 2005; Khan et al, 2007; Laliotis et al, 2007; Islam et al, 2008; Zolfaghari and Ross, 2009). …”

Section: Discussionmentioning

confidence: 99%

Multiple retinoic acid response elements cooperate to enhance the inducibility of CYP26A1 gene expression in liver

Zhang

Zolfaghari

Ross

2010

Gene

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CYP26A1, which catalyzes the oxidation of all-trans (at)-retinoic acid (RA), is induced moderately by RA in numerous tissues, but is highly responsive in liver. To understand this difference, we have examined the CYP26A1 gene sequence, identified multiple RA response elements (RAREs) and tested them functionally in HepG2 cells as model hepatocytes, and in the liver of vitamin A (VA)-adequate and -deficient rats. Analysis of a 2.2 kbp 5′-flanking region upstream of the CYP26A1 transcription start site (TSS) identified 3 conserved hexameric direct repeat-5 elements, RARE1, -2 and -3, and a half site, RARE4. The full-length promoter containing all 4 elements was sufficient and necessary to increase promoter activity similar to levels of endogenous CYP26A1 mRNA produced in HepG2 cells treated with at-RA. In DNA binding and chromatin immunoprecipitation assays, the binding of RARs to the proximal RARE1 and distal RARE2, -3, and -4 regions of the CYP26A1 promoter was increased in RA-treated HepG2 cells, and greater in VA-sufficient than VA-deficient liver. Moreover, RA increased the binding of RNA polymerase-II in the distal as well as the proximal region, indicating that the distal region may be looped to become positioned close to the TSS, a process favored by retinoic acid receptors. The results support a cooperative model in which the functioning of multiple RAREs may account for the strong inducibility of CYP26A1 in liver, which, in turn, may be important physiologically for restoring retinoid homeostasis when the concentration of RA rises.

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“…The presence of RAREs of the DR2 and DR5 types in the promoter region of retinoid-responsive genes are often a signature for those genes that respond directly, and often very rapidly, to RA or to other retinoid receptor agonists [20, 29]. However, RA -activated RAR receptors may also interact with other nuclear receptors such as AP1 [30–33], NFκB [34, 35], SP1 [36–40], and Nrf2 [41], and these may also regulate the expression of genes containing DNA sequences for these proteins. Balmer and Blomhoff [19] reported that whereas about 530 genes are known to be regulated by RA, only a few dozen of have definitively been shown to be directly regulated by RA.…”

Section: Discussionmentioning

confidence: 99%

“…Whether these elements have any functional role in the expression of the gene remains to be determined. Specificity proteins including SP1 and SP3 which are expressed ubiquitously in many tissues including liver in both hepatocytes and stellate cells [45–48] have been shown to interact with RA nuclear receptors in expression of a few genes [36–40]. However, no specific pattern of interaction common to all these genes has been reported.…”

Section: Discussionmentioning

confidence: 99%

An essential set of basic DNA response elements is required for receptor-dependent transcription of the lecithin:retinol acyltransferase (Lrat) gene

Zolfaghari¹,

Ross²

2009

Archives of Biochemistry and Biophysics

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Lecithin:retinol acyltransferase (LRAT) is essential for vitamin A storage. Nuclear run-on assays demonstrated transcriptional regulation of the Lrat gene in vivo by all-trans-retinoic acid (RA) and other retinoids. Analysis of a 2.5 kb segment of rat genomic DNA revealed that the region ~300 bp upstream from the transcription start site (TSS) is necessary for high luciferase (Luc) reporter activity in HEK293T and HepG2 cells. Although this region lacks retinoid receptor binding elements, it responded to the nuclear receptors RARα, RARβ or RARγ, with RXRα, with and without ligand. Removal of -111 bp from the TSS, which is well conserved in human, rat and mouse genomes, completely eliminated activity. This region contains several basic elements (TATA box, SP3 site, AP-1 site, CAAT box), all of which were essential. Nuclear extracts from RA-treated cells exhibited enhanced binding. Therefore, this proximal region together with basal transcription factors may be sufficient to drive Lrat expression.

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Cloning, characterization and computational analysis of the 5′ regulatory region of ovine glucose 6-phosphate dehydrogenase gene

Cited by 14 publications

References 36 publications

The expression level of non-alcoholic fatty liver disease-related gene PNPLA3 in hepatocytes is highly influenced by hepatic lipid status

The expression level of non-alcoholic fatty liver disease-related gene PNPLA3 in hepatocytes is highly influenced by hepatic lipid status

Multiple retinoic acid response elements cooperate to enhance the inducibility of CYP26A1 gene expression in liver

An essential set of basic DNA response elements is required for receptor-dependent transcription of the lecithin:retinol acyltransferase (Lrat) gene

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