Cloning, characterization and tissue-specific expression of the antimicrobial peptide hepcidin from caspian trout (Salmo caspius) and the antibacterial activity of the synthetic peptide

Shirdel, Iman; Kalbassi, Mohammad Reza; Hosseinkhani, Saman; Paknejad, Hamed

doi:10.1016/j.fsi.2019.05.010

Cited by 28 publications

(11 citation statements)

References 54 publications

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“…To our knowledge, the existence and function of the secretory cell in teleost skin (such as mucous goblet cells, squamous cells, pigment cells, and so on) have been confirmed and provided the first line of defense against pathogen invasion ( 1 , 74 ). The mucus secreted by these cells contains a large number of antimicrobial substances ( 75 , 76 ). Previous studies have demonstrated that MOS can increase the LZ activity and bactericidal activity in the skin of greater amberjack ( 13 ).…”

Section: Discussionmentioning

confidence: 99%

Mannan Oligosaccharides Application: Multipath Restriction From Aeromonas hydrophila Infection in the Skin Barrier of Grass Carp (Ctenopharyngodon idella)

et al. 2021

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The objective of this study was to evaluate the efficacy of dietary Mannan oligosaccharides (MOS) supplementation on skin barrier function and the mechanism of on-growing grass carp (Ctenopharyngodon idella). Five hundred forty grass carp were fed for 60 days from the growing stage with six different levels of MOS diets (0, 200, 400, 600, 800, and 1,000 mg kg-1). At the end of the growth trial, the 14-day Aeromonas hydrophila challenge experiment has proceeded. The obtained data indicate that MOS could (1) decline skin lesion morbidity after being challenged by the pathogenic bacteria; (2) maintain physical barrier function via improving antioxidant ability, inhibiting excessive apoptosis, and strengthening the tight junction between the epithelial cell and the related signaling pathway (Nrf2/Keap1, p38MAPK, and MLCK); and (3) regulate immune barrier function by modulating the production of antimicrobial compound and expression of involved cytokines and the related signaling pathway (TOR and NFκB). Finally, we concluded that MOS supplementation reinforced the disease resistance and protected the fish skin barrier function from Aeromonas hydrophila infection.

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Section: Discussionmentioning

confidence: 99%

Mannan Oligosaccharides Application: Multipath Restriction From Aeromonas hydrophila Infection in the Skin Barrier of Grass Carp (Ctenopharyngodon idella)

et al. 2021

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“…Very few studies have been published concerning the immunomodulatory effects of hepcidin peptides in salmonids, despite that several hepcidins were identified in this group of fish [ 30 , 31 , 32 , 33 , 105 ]. As of the writing of this review, such immunomodulatory studies are limited to Caspian trout ( Salmo caspius ).…”

Section: Immunomodulatory Role Of Salmonid Ampsmentioning

confidence: 99%

“…As of the writing of this review, such immunomodulatory studies are limited to Caspian trout ( Salmo caspius ). Injection of 1 µg/g synthetic Caspian trout Hepc into healthy specimens induced the expression of pro-inflammatory cytokine genes in the spleen and kidney [ 105 ]. Twenty-four hours after injection, only interleukin 6 ( il6 ) and tnfa gene expressions were significantly increased in the spleen, whereas in the kidney, all pro-inflammatory cytokines tested ( il1b , il6 , and tnfa ) were significantly upregulated at the gene level [ 106 ].…”

Section: Immunomodulatory Role Of Salmonid Ampsmentioning

confidence: 99%

Antimicrobial Peptides of Salmonid Fish: From Form to Function

Brunner

Varga

Dixon

2020

Biology

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Antimicrobial peptides (AMPs) are small, usually cationic, and amphiphilic molecules that play a crucial role in molecular and cellular host defense against pathogens, tissue damage, and infection. AMPs are present in all metazoans and several have been discovered in teleosts. Some teleosts, such as salmonids, have undergone whole genome duplication events and retained a diverse AMP repertoire. Salmonid AMPs have also been shown to possess diverse and potent antibacterial, antiviral, and antiparasitic activity and are induced by a variety of factors, including dietary components and specific molecules also known as pathogen-associated molecular patterns (PAMPs), which may activate downstream signals to initiate transcription of AMP genes. Moreover, a multitude of cell lines have been established from various salmonid species, making it possible to study host-pathogen interactions in vitro, and several of these cell lines have been shown to express various AMPs. In this review, the structure, function, transcriptional regulation, and immunomodulatory role of salmonid AMPs are highlighted in health and disease. It is important to characterize and understand how salmonid AMPs function as this may lead to a better understanding of host-pathogen interactions with implications for aquaculture and medicine.

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“…Orange-spotted grouper (Epinephelus coioides) derived from AMP EPI is effective against GPB (Su and Chen, 2020). Cysteine-rich Hepcidins (CtHep) from vertebrates such as fish, reptiles, and amphibians can significantly inhibit Streptococcus iniae and A. hydrophila (Shirdel et al, 2019). Marine betta fish Betta splendens produce four families of AMPs, including defensins, piscidins, hepcidins, and LEAP-2, which vigorously suppress the growth of fungi, bacteria, virus, and parasites (Amparyup et al, 2020).…”

Section: Antimicrobial Peptides From Marine Vertebratesmentioning

confidence: 99%

Antimicrobial Peptides: Novel Source and Biological Function With a Special Focus on Entomopathogenic Nematode/Bacterium Symbiotic Complex

et al. 2021

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The rapid emergence of multidrug resistant microorganisms has become one of the most critical threats to public health. A decrease in the effectiveness of available antibiotics has led to the failure of infection control, resulting in a high risk of death. Among several alternatives, antimicrobial peptides (AMPs) serve as potential alternatives to antibiotics to resolve the emergence and spread of multidrug-resistant pathogens. These small proteins exhibit potent antimicrobial activity and are also an essential component of the immune system. Although several AMPs have been reported and characterized, studies associated with their potential medical applications are limited. This review highlights the novel sources of AMPs with high antimicrobial activities, including the entomopathogenic nematode/bacterium (EPN/EPB) symbiotic complex. Additionally, the AMPs derived from insects, nematodes, and marine organisms and the design of peptidomimetic antimicrobial agents that can complement the defects of therapeutic peptides have been used as a template.

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Cloning, characterization and tissue-specific expression of the antimicrobial peptide hepcidin from caspian trout (Salmo caspius) and the antibacterial activity of the synthetic peptide

Cited by 28 publications

References 54 publications

Mannan Oligosaccharides Application: Multipath Restriction From Aeromonas hydrophila Infection in the Skin Barrier of Grass Carp (Ctenopharyngodon idella)

Mannan Oligosaccharides Application: Multipath Restriction From Aeromonas hydrophila Infection in the Skin Barrier of Grass Carp (Ctenopharyngodon idella)

Antimicrobial Peptides of Salmonid Fish: From Form to Function

Antimicrobial Peptides: Novel Source and Biological Function With a Special Focus on Entomopathogenic Nematode/Bacterium Symbiotic Complex

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