Cloning, chromosomal localization, SNP detection and association analysis of the porcine IRS-1 gene

Niu, P.X.; Huang, Zhenyu; Li, C.-C.; Fan, Bin; Li, K.; Liu, B.; Yu, Mei; Song, Zhao

doi:10.1007/s11033-008-9420-3

Cited by 7 publications

(2 citation statements)

References 20 publications

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“…Moreover, the IRS1 gene (insulin receptor substrate 1) protein, which showed downregulated expression in our study compared with the reference value, is associated with the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) pathway and also PI3K/AKT ( Figure A5 ) [ 74 , 75 ]. IRS1 affects cell metabolism regulation, survival, and apoptosis [ 76 ]. The association of its overexpression with GCs proliferation was demonstrated in PCOS (polycystic ovary syndrome) study, where it activated the MAPK/ERK pathway and promoted the disorder [ 77 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Profile of New Gene Markers Encoding Proteins Responsible for Structure of Porcine Ovarian Granulosa Cells

Kulus

Kranc

et al. 2021

Biology

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The extracellular matrix (ECM) in granulosa cells is functionally very important, and it is involved in many processes related to ovarian follicle growth and ovulation. The aim of this study was to describe the expression profile of genes within granulosa cells that are associated with extracellular matrix formation, intercellular signaling, and cell–cell fusion. The material for this study was ovaries of sexually mature pigs obtained from a commercial slaughterhouse. Laboratory-derived granulosa cells (GCs) from ovarian follicles were cultured in a primary in vitro culture model. The extracted genetic material (0, 48, 96, and 144 h) were subjected to microarray expression analysis. Among 81 genes, 66 showed increased expression and only 15 showed decreased expression were assigned to 7 gene ontology groups “extracellular matrix binding”, “extracellular matrix structural constituent”, “binding, bridging”, “cadherin binding”, “cell adhesion molecule binding”, “collagen binding” and “cadherin binding involved in cell-cell adhesion”. The 10 genes with the highest expression (POSTN, ITGA2, FN1, LAMB1, ITGB3, CHI3L1, PCOLCE2, CAV1, DCN, COL14A1) and 10 of the most down-regulated (SPP1, IRS1, CNTLN, TMPO, PAICS, ANK2, ADAM23, ABI3BP, DNAJB1, IGF1) were selected for further analysis. The results were validated by RT-qPCR. The current results may serve as preliminary data for further analyses using in vitro granulosa cell cultures in assisted reproduction technologies, studies of pathological processes in the ovary as well as in the use of the stemness potential of GCs.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic Profile of New Gene Markers Encoding Proteins Responsible for Structure of Porcine Ovarian Granulosa Cells

Kulus

Kranc

et al. 2021

Biology

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“…The IRS1 gene belongs to the IRS family, which is known to control postnatal growth (Pete et al, 1999 ; Baroni et al, 2001 ), and knocked-out IRS1 mice exhibit severely retarded growth (Araki et al, 1994 ). The IRS1 gene was reported to affect growth and carcass traits in rabbits (Helal et al, 2022 ; Megahed et al, 2023 ; Safaa et al, 2023 ), sheep (Zhou et al, 2021 ), pigs (Niu et al, 2009 ), and beef cattle (Wu et al 2014b ).…”

Section: Introductionmentioning

confidence: 99%

Candidate genes associated with reproductive traits in rabbits

Helal,

Sameh,

Gharib

et al. 2024

Trop Anim Health Prod

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In the era of scientific advances and genetic progress, opportunities in the livestock sector are constantly growing. The application of molecular-based methods and approaches in farm animal breeding would accelerate and improve the expected results. The current work aims to comprehensively review the most important causative mutations in candidate genes that affect prolificacy traits in rabbits. Rabbits are a source of excellent-tasting meat that is high in protein and low in fat. Their early maturity and intensive growth are highly valued all over the world. However, improving reproductive traits and prolificacy in rabbits could be very tricky with traditional selection. Therefore, traditional breeding programs need new methods based on contemporary discoveries in molecular biology and genetics because of the complexity of the selection process. The study and implementation of genetic markers related to production in rabbits will help to create populations with specific productive traits that will produce the desired results in an extremely short time. Many studies worldwide showed an association between different genes and productive traits in rabbits. The study of these polymorphisms and their effects could be useful for molecular-oriented breeding, particularly marker-assisted selection programs in rabbit breeding.

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MyoD control of SKIP expression during pig skeletal muscle development

et al. 2010

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Skeletal muscle and kidney enriched inositol phosphatase (SKIP) was identified as a 5'-inositol phosphatase that hydrolyzes PI(3,4,5)P3 to PI(3,4)P2 that negatively regulates insulin-induced phosphatidylinositol 3-kinase signaling in skeletal muscle. In this study, we obtained a 1575-bp mRNA sequence of porcine SKIP that included the full coding region encoding a protein of 450 amino acids. With the use of comparative mapping, we mapped this gene to SSC12 q1.3, where many QTLs affect Backfat thickness at 10th rib, carcass yield, the number of muscle fibers, and ham weight traits. As a candidate gene for growth and carcass traits, a novel single nucleotide polymorphism in exon 12 (G>A) was detected by PCR-RFLP. The results showed that the GG genotype had higher skin percentage (SP), carcass length to first spondyle (CL1), carcass length to first rib (CL2), but lower intramuscular fat (IMF) as compared with genotype AG (P<0.05), and allele G seemed to be associated with an increase in the growth trait. Porcine SKIP was expressed abundantly in skeletal muscle tissue and was transcriptionally upregulated during skeletal muscle differentiation. Analysis of the porcine SKIP promoter sequence demonstrated that MyoD was involved in regulating SKIP mRNA expression in myotubes, partly via the cis-acting elements in SKIP promoter. In summary, we suggested that SKIP might play a role in the regulation of skeletal muscle development in pigs.

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Cloning, chromosomal localization, SNP detection and association analysis of the porcine IRS-1 gene

Cited by 7 publications

References 20 publications

Transcriptomic Profile of New Gene Markers Encoding Proteins Responsible for Structure of Porcine Ovarian Granulosa Cells

Transcriptomic Profile of New Gene Markers Encoding Proteins Responsible for Structure of Porcine Ovarian Granulosa Cells

Candidate genes associated with reproductive traits in rabbits

MyoD control of SKIP expression during pig skeletal muscle development

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