Cloning, expression and antioxidant activity of a novel collagen from Pelodiscus sinensis

Xu, Ran; Li, Dengfeng; Peng, Jiao; Fang, Jing; Zhang, Liping; Liu, Lianguo

doi:10.1007/s11274-016-2057-9

Cited by 8 publications

(5 citation statements)

References 24 publications

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“…The DPPH radical scavenging activity of fluorescent phycocyanin was tested according to the method of Xu et al [ 32 ], with some modifications. The purified recombinant protein was diluted with KPB to 50, 100, 150, 200, and 250 µg/mL.…”

Section: Methodsmentioning

confidence: 99%

Biosynthesis of Fluorescent β Subunits of C-Phycocyanin from Spirulina subsalsa in Escherichia coli, and Their Antioxidant Properties

Yang

Chen

et al. 2018

Molecules

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Phycocyanin, which covalently binds phycocyanobilin chromophores, is not only a candidate fluorescent probe for biological imaging, but also a potential antioxidative agent for healthcare. Herein, a plasmid harboring two cassettes was constructed, with cpcB from Spirulina subsalsa in one cassette and the fusion gene cpcS::ho1::pcyA in the other, and then expressed in Escherichia coli. PCB-CpcB(C-82), a fluorescent phycocyanin β subunit, was biosynthesized in E. coli, exhibiting an absorption maximum at 620 nm and fluorescence emission maximum at 640 nm. When cpcS was replaced by cpcT, PCB-CpcB(C-153), another fluorescent phycocyanin β subunit, was produced, exhibiting an absorption maximum at 590 nm and fluorescence emission maximum at 620 nm. These two fluorescent biliproteins showed stronger scavenging activity toward hydroxyl and DPPH free radicals than apo-CpcB. The IC50 values for hydroxyl radical scavenging by PCB-CpcB(C-82), PCB-CpcB(C-153), and apo-CpcB were 38.72 ± 2.48 µg/mL, 51.06 ± 6.74 µg/mL, and 81.82 ± 0.67 µg/mL, respectively, and the values for DPPH radical scavenging were 201.00 ± 5.86 µg/mL, 240.34 ± 4.03 µg/mL, and 352.93 ± 26.30 µg/mL, respectively. The comparative antioxidant capacities of the proteins were PCB-CpcB(C-82) > PCB-CpcB(C-153) > apo-CpcB, due to bilin binding. The two fluorescent biliproteins exhibited a significant effect on relieving the growth of E. coli cells injured by H2O2. The results of this study suggest that the fluorescent phycocyanin β subunits of S. subsalsa were reconstructed by one expression vector in E. coli, and could be developed as potential antioxidants.

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Section: Methodsmentioning

confidence: 99%

Biosynthesis of Fluorescent β Subunits of C-Phycocyanin from Spirulina subsalsa in Escherichia coli, and Their Antioxidant Properties

Yang

Chen

et al. 2018

Molecules

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“…The soft-shelled-turtle (Pelodiscus sinensis) is a commercially important aquatic species rich in collagen. Investigations conducted by the researchers Xu et al (2016), demonstrated the antioxidant activity with the elimination of free radicals in approximately 98.9% from a new collagen extracted from P. sinensis. More recent studies have also demonstrated that antioxidant enzymes, peroxiredoxins, isolated softshelled-turtle have been shown to influence the defense against oxidative and modular stress in the immunohistochemical response in vitro (Zhang et al, 2019).…”

Section: Determination Of Lbfpe's Antioxidant Activitymentioning

confidence: 98%

Antibacterial, total phenols, antioxidant, and fatty acids of the lyophilized body fat of Podocnemis expansa (Schweigger, 1812) from farm in Acre State, Brazil

Da¹,

De²,

Ferraz³

et al. 2020

J. Med. Plants Res.

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Podocnemis expansa (Podocnemididae) is a chelonian of suborder Pleurodira and is popularly known as Amazon turtle. The animal's body fat was removed from the farm in Xapuri city in Acre State, Brazil. The fat was lyophilized and the oil was extracted by the Soxhlet method using hexane, as a solvent. The fatty acids majority, identified and quantified by gas chromatography-flame ionization detector, were: Arachidonic (25.2%), oleic (ω9, 17.6%), palmitic (ω7, 13.5%), stearic (11.9%) and linoleic (ω6, 9.5%). These, are essential for human health and can be helpful in the treatment of cardiovascular diseases, arthritis, psoriasis, improving symptoms of depression, Alzheimer's disease, among others therapies and pathologies. The total phenolic compounds (377.52%) and the antioxidant activity (88.64%) determined in this investigation indicate its high potential in preventing lower risks of cardiovascular and other chronic diseases. Therefore, the results demonstrate the pharmacological, therapeutic and nutraceutical potential of the main chemical constituents of these substances for prospecting and developing biologically active and functional molecules to expand scientific investigations and the commercial chemical industry.

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“…A total of 400 µM Isopropyl-β-D-thiogalactopyranoside (IPTG) was added to the medium to induce expression, which was maintained at 20 • C. Bacteria were collected by centrifugation and ultrasonically crushed to obtain a crude extract. The His-tagged proteins were subsequently isolated using a His GraviTrap column (Cytiva, Marlborough, MA, USA) and concentrated using an ultrafiltration filter (Millipore, St. Louis, MO, USA) to obtain the target proteins [46]. The detection of biolayer interferometry was performed as previously described [47,48].…”

Section: Expression and Purification Of Rta And Its Mutantsmentioning

confidence: 99%

Identification and Biological Evaluation of a Novel Small-Molecule Inhibitor of Ricin Toxin

Yang,

Wei,

Cao

et al. 2024

Molecules

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The plant-derived toxin ricin is classified as a type 2 ribosome-inactivating protein (RIP) and currently lacks effective clinical antidotes. The toxicity of ricin is mainly due to its ricin toxin A chain (RTA), which has become an important target for drug development. Previous studies have identified two essential binding pockets in the active site of RTA, but most existing inhibitors only target one of these pockets. In this study, we used computer-aided virtual screening to identify a compound called RSMI-29, which potentially interacts with both active pockets of RTA. We found that RSMI-29 can directly bind to RTA and effectively attenuate protein synthesis inhibition and rRNA depurination induced by RTA or ricin, thereby inhibiting their cytotoxic effects on cells in vitro. Moreover, RSMI-29 significantly reduced ricin-mediated damage to the liver, spleen, intestine, and lungs in mice, demonstrating its detoxification effect against ricin in vivo. RSMI-29 also exhibited excellent drug-like properties, featuring a typical structural moiety of known sulfonamides and barbiturates. These findings suggest that RSMI-29 is a novel small-molecule inhibitor that specifically targets ricin toxin A chain, providing a potential therapeutic option for ricin intoxication.

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Cloning, expression and antioxidant activity of a novel collagen from Pelodiscus sinensis

Cited by 8 publications

References 24 publications

Biosynthesis of Fluorescent β Subunits of C-Phycocyanin from Spirulina subsalsa in Escherichia coli, and Their Antioxidant Properties

Biosynthesis of Fluorescent β Subunits of C-Phycocyanin from Spirulina subsalsa in Escherichia coli, and Their Antioxidant Properties

Antibacterial, total phenols, antioxidant, and fatty acids of the lyophilized body fat of Podocnemis expansa (Schweigger, 1812) from farm in Acre State, Brazil

Identification and Biological Evaluation of a Novel Small-Molecule Inhibitor of Ricin Toxin

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