Cloning, Expression, and Characterization of the hxk-1 Gene from the White Truffle Tuber borchii Vittad.: A First Step toward Understanding Sugar Metabolism

Agostini, Deborah; Polidori, Emanuela; Palma, Francesco; Ceccaroli, Paola; Saltarelli, Roberta; Tonelli, Daniela; Stocchi, Vilberto

doi:10.1006/fgbi.2001.1268

Cited by 7 publications

(3 citation statements)

References 23 publications

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“…, 1999). The first gene found in the T. melanosporum genome, Tmelhxk , codes for a protein showing the highest similarity with T. borchii Hxk‐1 (AF309088, 94% identity) (Agostini et al. , 2001) and with several fungal hexokinases, such as Botryotinia fuckeliana (BC1G_12086, 68% identity) and N. crassa (XP_965673.2 or NCU02542, 66% identity).…”

Section: Resultsmentioning

confidence: 99%

“…In S. cerevisiae, two hexokinase isoenzymes and a glucokinase are present, in N. crassa, three hexokinases and a glucokinase have been described (Borkovich et al, 2004), and, in T. borchii, three distinct forms of hexokinase have been identified during mycelial growth (Ceccaroli et al, 1999). The first gene found in the T. melanosporum genome, Tmelhxk, codes for a protein showing the highest similarity with T. borchii Hxk-1 (AF309088, 94% identity) (Agostini et al, 2001) and with several fungal hexokinases, such as Botryotinia fuckeliana (BC1G_12086, 68% identity) and N. crassa (XP_965673.2 or NCU02542, 66% identity). The second gene, Tmelhxk2, shares maximum identity (39%) with an N. crassa hypothetical protein (NCU06996) and with several putative hexokinase family proteins XprF, which are purely regulatory in terms of function and play a role in the response to carbon starvation in the multicellular fungus Aspergillus nidulans (Bernardo et al, 2007).…”

Section: Hexose Catabolismmentioning

confidence: 99%

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Genomic profiling of carbohydrate metabolism in the ectomycorrhizal fungus Tuber melanosporum

et al. 2010

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Summary• Primary carbohydrate metabolism plays a special role related to carbon ⁄ nitrogen exchange, as well as metabolic support of fruiting body development, in ectomycorrhizal macrofungi. In this study, we used information retrieved from the recently sequenced Tuber melanosporum genome, together with transcriptome analysis data and targeted validation experiments, to construct the first genomewide catalogue of the proteins supporting carbohydrate metabolism in a plantsymbiotic ascomycete.• More than 100 genes coding for enzymes of the glycolysis, pentose phosphate, tricarboxylic acid, glyoxylate and methylcitrate pathways, glycogen, trehalose and mannitol metabolism and cell wall precursor were annotated. Transcriptional regulation of these pathways in different stages of the T. melanosporum lifecycle was investigated using whole-genome oligoarray expression data together with real-time reverse transcription-polymerase chain reaction analysis of selected genes.• The most significant results were the identification of methylcitrate cycle genes and of an acid invertase, the first enzyme of this kind to be described in a plantsymbiotic filamentous fungus.• A subset of transcripts coding for trehalose, glyoxylate and methylcitrate enzymes was up-regulated in fruiting bodies, whereas genes involved in mannitol and glycogen metabolism were preferentially expressed in mycelia and ectomycorrhizas, respectively. These data indicate a high degree of lifecycle stage specialization for particular branches of carbohydrate metabolism in T. melanosporum.

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Section: Resultsmentioning

confidence: 99%

Section: Hexose Catabolismmentioning

confidence: 99%

Genomic profiling of carbohydrate metabolism in the ectomycorrhizal fungus Tuber melanosporum

et al. 2010

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“…In the literature, very little is known about the early metabolism of glucose and fructose in truffles, but there is much evidence that sugars are not only important energy sources and structural components, but in higher plants they play also a role of signal molecules (Jang et al, 1997). Recently, in an attempt to extend our knowledge on sugar metabolism in Tuber species, we cloned, expressed and characterized the hxk-1 gene from T. borchii mycelium (Agostini et al, 2001). Although glycolysis appears to be a general pathway in all organisms, remarkable differences have been observed for the enzymes involved in hexose utilization and their regulation.…”

Section: Introductionmentioning

confidence: 98%