Cloning, expression and characterization of L-cysteine desulfhydrase gene from Pseudomonas sp. TS1138

Bai, Gang; Yu, Yang; Yang, Li; Jin, Yongjie; Liu, Chunqin

doi:10.1007/s11515-007-0059-6

Cited by 3 publications

(3 citation statements)

References 18 publications

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“…On the other hand, Cys also may break down to release sulfide in bacteria through the enzyme cysteine desulfhydrase (EC: 4.4.1.15) [ 103 , 104 , 105 , 106 ]. The gene encoding this enzyme ( dcyD ) was found in 16 strains, not including the P. aeruginosa , P. deceptionensis , Pseudomonas sp.…”

Section: Resultsmentioning

confidence: 99%

Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source

Gallardo-Benavente

Campo-Giraldo

Castro‐Severyn

et al. 2021

Genes

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Here, we present the draft genome sequence of Pseudomonas sp. GC01, a cadmium-resistant Antarctic bacterium capable of biosynthesizing CdS fluorescent nanoparticles (quantum dots, QDs) employing a unique mechanism involving the production of methanethiol (MeSH) from methionine (Met). To explore the molecular/metabolic components involved in QDs biosynthesis, we conducted a comparative genomic analysis, searching for the genes related to cadmium resistance and sulfur metabolic pathways. The genome of Pseudomonas sp. GC01 has a 4,706,645 bp size with a 58.61% G+C content. Pseudomonas sp. GC01 possesses five genes related to cadmium transport/resistance, with three P-type ATPases (cadA, zntA, and pbrA) involved in Cd-secretion that could contribute to the extracellular biosynthesis of CdS QDs. Furthermore, it exhibits genes involved in sulfate assimilation, cysteine/methionine synthesis, and volatile sulfur compounds catabolic pathways. Regarding MeSH production from Met, Pseudomonas sp. GC01 lacks the genes E4.4.1.11 and megL for MeSH generation. Interestingly, despite the absence of these genes, Pseudomonas sp. GC01 produces high levels of MeSH. This is probably associated with the metC gene that also produces MeSH from Met in bacteria. This work is the first report of the potential genes involved in Cd resistance, sulfur metabolism, and the process of MeSH-dependent CdS QDs bioproduction in Pseudomonas spp. strains.

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Section: Resultsmentioning

confidence: 99%

Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source

Gallardo-Benavente

Campo-Giraldo

Castro‐Severyn

et al. 2021

Genes

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“…In Pseudomonas sp. TS1138, only one CD gene was identifi ed and functionally characterized (Yu et al, 2007b). Elimination of the gene product from crude enzyme extracts of the cells resulted in a high L-cysteine production (Yu et al, 2007a).…”

Section: Strain Improvement By Uv-mutagenesismentioning

confidence: 99%

“…Consequently, the whole racemic ATC is converted to L-cysteine. However, the effi ciency of this bioconversion process is limited, presumably due to limited production of the enzymes involved in the bioconversion and the presence of the cysteine-degrading enzymes, such as cysteine desulfhydrase in the host (Ryu et al, 1997;Sano and Mitsugi, 1978;Tamura et al, 2001;Yu et al, 2007b).…”

Section: Introductionmentioning

confidence: 99%

Isolation and genetic improvement of Pseudomonas sp. strain HUT-78, capable of enzymatic production of L-cysteine from DL-2-amino-Δ2-thiazoline-4-carboxylic acid

Yang

Liu

Deng

et al. 2011

J. Gen. Appl. Microbiol.

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Microorganisms able to bioconvert DL-2-amino-Δ 2 -thiazoline-4-carboxylic acid (DL-ATC) into Lcysteine were originally isolated from 10 soil samples with DL-ATC as the sole nitrogen source. Ninety-seven L-cysteine-producing bacterial strains were screened out and obtained in pure culture. Among them, a strain, designated as HUT-78, was selected as the best producer, with a molar bioconversion rate of 60%. Based on the 16S rRNA gene sequence analysis, this isolate was placed within the genus Pseudomonas. A novel mutant of this strain with a signifi cantly reduced activity of L-cysteine desulfhydrase, a L-cysteine-decomposing enzyme, was derived by UV-mutagenesis. This mutant, designated as mHUT-78, exhibited a 42% increase in L-cysteine producing activity. Moreover, the bioconversion reactions in both the parent and the mutant strain were signifi cantly accelerated by co-overexpression of the two key enzymes, AtcB and AtcC, involved in the bioconversion reaction.

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Effects of sulphate-deficiency on cysteine metabolism in the green alga Chlorella sorokiniana

Salbitani¹

2010

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Cloning, expression and characterization of L-cysteine desulfhydrase gene from Pseudomonas sp. TS1138

Cited by 3 publications

References 18 publications

Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source

Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source

Isolation and genetic improvement of Pseudomonas sp. strain HUT-78, capable of enzymatic production of L-cysteine from DL-2-amino-Δ2-thiazoline-4-carboxylic acid

Effects of sulphate-deficiency on cysteine metabolism in the green alga Chlorella sorokiniana

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