Cloning, molecular characterization, and spatial and developmental expression analysis of GPR41 and GPR43 genes in New Zealand rabbits

Fu, Chunyan; Liu, L.; Qingrong, Gao; Sui, Xinxia; Li, F.C.

doi:10.1017/s175173111700043x

Cited by 18 publications

(9 citation statements)

References 29 publications

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“…GPR41 and 43 are highly expressed in adipose tissue of pigs and mice [42,51]. In our study, we found also high expression of GPR41 and 43 in adipose but did not detect the GPR41 and 43 genes in liver and muscle, which is in agreement with the findings of Fu et al [52]. In 3T3-L1 cells, GPR43 blocking inhibited the PPARγ transcription [53].…”

Section: Discussionsupporting

confidence: 91%

Acetate Affects the Process of Lipid Metabolism in Rabbit Liver, Skeletal Muscle and Adipose Tissue

Liu

2019

Animals

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Simple SummaryLots of short-chain fatty acids (SCFAs) are produced in the rabbit cecum after dietary fiber fermentation. In addition to supplying energy, SCFAs could regulate lipid metabolism, but the related mechanism is still unknown. In our experiment, we study the effect of acetate (major SCFAs, 70–80%) on rabbit lipid metabolism. The present study found that acetate alters the process of lipid metabolism in rabbit liver, skeletal muscle and adipose tissue, and inferred some signaling pathways related to the process. A mechanism of acetate-regulating lipid metabolism is useful to identify the function in fat metabolism of microbiological products from rabbit and rabbit processes for nutrition metabolism.AbstractShort-chain fatty acids (SCFAs) (a microbial fermentation production in the rabbit gut) have an important role in many physiological processes, which may be related to the reduced body fat of rabbits. In the present experiment, we study the function of acetate (a major SCFA in the rabbit gut) on fat metabolism. Ninety rabbits (40 days of age) were randomly divided into three groups: a sham control group (injection of saline for four days); a group experiencing subcutaneous injection of acetate for four days (2 g/kg BM per day, one injection each day, acetate); and a pair-fed sham treatment group. The results show that acetate-inhibited lipid accumulation by promoting lipolysis and fatty acid oxidation and inhibiting fatty acid synthesis. Activated G protein-coupled receptor 41/43, adenosine monophosphate activated protein kinase (AMPK) and extracellular-signal-regulated kinase (ERK) 1/2 signal pathways were likely to participate in the regulation in lipid accumulation of acetate. Acetate reduced hepatic triglyceride content by inhibiting fatty acid synthesis, enhancing fatty acid oxidation and lipid output. Inhibited peroxisome proliferator-activated receptor α (PPARα) and activated AMPK and ERK1/2 signal pathways were related to the process in liver. Acetate reduced intramuscular triglyceride level via increasing fatty acid uptake and fatty acid oxidation. PPARα was associated with the acetate-reduced intracellular fat content.

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Section: Discussionsupporting

confidence: 91%

Acetate Affects the Process of Lipid Metabolism in Rabbit Liver, Skeletal Muscle and Adipose Tissue

Liu

2019

Animals

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“…We extracted total RNA, from intestinal tissue by the TRIzol method, and then reverse transcribed it with the kit provided by Beijing TransGen Biology Co., Ltd. and finally obtained cDNA. Then, we used the kit provided by Hunan Accurate Biological Co., Ltd. to perform RT‐PCR on a Swiss Roche LightCycler 96 real‐time fluorescence quantitative PCR instrument (Fu et al., 2017, 2018; Liu et al., 2017). Finally, we used the 2 −ΔΔCt method to calculate the expression of related genes.…”

Section: Methodsmentioning

confidence: 99%

Effect of sodium butyrate on slaughter performance, serum indexes and intestinal barrier of rabbits

Chen

Zhao

et al. 2021

Animal Physiology Nutrition

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The purpose of this study was to investigate the effect of sodium butyrate on slaughter performance, serum indexes and the intestinal barrier in rabbits. Six hundred healthy weaned rabbits were randomly divided into three groups (5 replicates per group, 40 rabbits per replicate): control (fed a basal diet), sodium butyrate (fed a basal diet containing 0.5% sodium butyrate) and antibiotic (fed a basal diet containing 0.004% antibiotic). The trial lasted 35 days, including 7 days of pretesting and 28 days of formal testing. The results showed that dietary sodium butyrate supplementation increased the full‐bore weight, the half‐bore weight and the half‐bore rate of rabbits. Meanwhile, the content of aspartate aminotransferase (AST) in serum was increased in rabbits fed the sodium butyrate diet. According to the intestinal barrier, after adding sodium butyrate to feed, the tight junction function of the rabbit intestine is enhanced, and the intestinal microbial composition is also improved. To sum up, after sodium butyrate was added to feed instead of antibiotics, slaughter performance was significantly enhanced, serum indexes were improved, and intestinal barrier function was also enhanced. Therefore, sodium butyrate can be added to feed as an additive and can replace antibiotics.

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“…In swine, FFAR2 expresses in the heart [13], liver [13], spleen [13,86], pancreas [86], adipose tissues [13,86,87], kidney [13], small intestine [88], caecum [88], colon [88], and skeletal muscle [13]. In sheep, FFAR2 expresses in abomasal (distal gastric) lymph nodes [89]. In New Zealand rabbits FFAR2 expresses in thymus, spleen, pancreas, adipose tissue, lungs, duodenum, jejunum, cecum ileum and colon [90].…”

Section: Expression Of Ffar2 and Ffar3 In Different Species And Tissues/cellsmentioning

confidence: 99%

Free Fatty Acid Receptors 2 and 3 as Microbial Metabolite Sensors to Shape Host Health: Pharmacophysiological View

et al. 2020

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The role of the gut microbiome in human health is becoming apparent. The major functional impact of the gut microbiome is transmitted through the microbial metabolites that are produced in the gut and interact with host cells either in the local gut environment or are absorbed into circulation to impact distant cells/organs. Short-chain fatty acids (SCFAs) are the major microbial metabolites that are produced in the gut through the fermentation of non-digestible fibers. SCFAs are known to function through various mechanisms, however, their signaling through free fatty acid receptors 2 and 3 (FFAR2/3; type of G-coupled protein receptors) is a new therapeutic approach. FFAR2/3 are widely expressed in diverse cell types in human and mice, and function as sensors of SCFAs to change several physiological and cellular functions. FFAR2/3 modulate neurological signaling, energy metabolism, intestinal cellular homeostasis, immune response, and hormone synthesis. FFAR2/3 function through Gi and/or Gq signaling, that is mediated through specific structural features of SCFAs-FFAR2/3 bindings and modulating specific signaling pathway. In this review, we discuss the wide-spread expression and structural homologies between human and mice FFAR2/3, and their role in different human health conditions. This information can unlock opportunities to weigh the potential of FFAR2/3 as a drug target to prevent human diseases.

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Cloning, molecular characterization, and spatial and developmental expression analysis of GPR41 and GPR43 genes in New Zealand rabbits

Cited by 18 publications

References 29 publications

Acetate Affects the Process of Lipid Metabolism in Rabbit Liver, Skeletal Muscle and Adipose Tissue

Acetate Affects the Process of Lipid Metabolism in Rabbit Liver, Skeletal Muscle and Adipose Tissue

Effect of sodium butyrate on slaughter performance, serum indexes and intestinal barrier of rabbits

Free Fatty Acid Receptors 2 and 3 as Microbial Metabolite Sensors to Shape Host Health: Pharmacophysiological View

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