Cloning of cDNA encoding the murine IgG1 induction factor by a novel strategy using SP6 promoter

Abstract

Complementary DNA encoding the IgG1 induction factor, the first lymphokine directed to B lymphocytes, from a murine T-cell line has been cloned using a new strategy. The putative primary amino-acid sequence was deduced from the nucleotide sequence determined. The lymphokine synthesized by the direction of this cloned cDNA has many other functions, such as production of B-cell growth factor-1 and induction of Ia on B cells.

Interleukin‐4 stimulates rheumatoid synovial fibroblasts to express matrix metalloproteinase‐1 (tissue collagenase) and histamine H1 receptor mRNA

“…Subsequent to biochemical purification (27) and molecular cloning (28,29), it was reported that II.,-4 and IgG1 enhancing factor, also called B cell differentiation factor-,/ (BCDF-~,), arc the same lymphokine (30). IL-4 was later shown to act also on T cells as well as on other cell types including monocytes, endothelial cells, and fibroblasts (13,31).…”

Studies on synthesis of interleukin-6 and gammaglobulin in peripheral blood mononuclear cells of patients with systemic lupus erythematosus.

“…Recently, evidence has been accumulated to show that the growth of B cells and their differentiation into antibody producing cells require several soluble factors. Three factors regulating the B cell response have been reported in the human ; interleukin-4 (IL-4) for the early activation of resting B cells (Noma et al 1986; Yokota et al 1986), IL-5 for the growth of activated B cells , and IL-6 for the final differentiation of B cells into antibody producing cells (Hirano et al 1985(Hirano et al , 1986. It has been reported that cardiac myxoma patients showed autoimmune symptoms, hypergammaglobulinemia, and autoantibody production which could be mediated by a large amount of IL-6 released from myxoma .…”

Calcium mobilization by angiotensin II and neurotransmitter receptors expressed in Xenopus laevis oocytes

“…No responses to the transmitters were observed under these conditions in oocytes injected with aequorin alone This approach offers several advantages over assay techniques which are currently being used to clone Ca 2 +-mobilizing receptors [ 15,17,18]. The present method is rapid and convenient; no radioisotopes are required and no electrophysiological expertise or equipment is necessary.…”