Cloning of cDNA for a cell wall-bound acid invertase from tomato (Lycopersicon esculentum) and expression of soluble and cell wall-bound invertases in plants and wounded leaves of L. esculentum and L. peruvianum.

Abstract

A cDNA, Wiv-1, for an isozyme of acid invertase (EC 3.2.1.26) was cloned from wounded leaves of tomato (Lycopersicon esculentum). The encoded protein had a basic isoelectric point and strong similarity to the amino acid sequences of plant cell wall-bound invertases. The conserved sequence WECPD that is found in all plant cell wall-bound invertases was also found in the deduced protein. These results suggested that Wiv-1 encoded a cell wall-bound acid invertase of tomato. Wounding increased the levels of mRNAs for soluble and cell wall-bound invertases and the activities of these invertases in leaves of L. esculentum and of a related species, L. peruvianum. The induction of Aiv-1 mRNA for the soluble enzyme in wounded leaves was not very strong, while that of Wiv-1 mRNA for the wall-bound enzyme was prominent. The level of Aiv-1 mRNA reached a maximum 48 h after wounding while that of Wiv-1 mRNA continued to rise for up to 96 h. These findings suggested that the genes for the two isozymes responded independently to wounding. The levels in various organs of Aiv-1 and Wiv-1 mRNAs were higher in L. esculentum than in L. peruvianum. Possible roles of cell wall-bound acid invertase in wound response and in developing plant are discussed.

Temporal Changes in Allocation and Partitioning of New Carbon as 11C Elicited by Simulated Herbivory Suggest that Roots Shape Aboveground Responses in Arabidopsis

“…In Arabidopsis, invertases are not only the main route of Suc metabolism but are essential for normal plant growth and development (Barratt et al, 2009). Invertases also play a crucial role in regulating the supply of photosynthate to naturally occurring sink tissues (Tang et al, 1999;Weschke et al, 2003;Heyer et al, 2004;Roitsch and González, 2004) and are known to be up-regulated during gall formation (Rehill and Schultz, 2003;Siemens et al, 2011) and after wounding and insect attack (Zhang et al, 1996;Ohyama et al, 1998;Rosenkranz et al, 2001;Arnold and Schultz, 2002).…”

Metabolism and Accumulation of Sugars Translocated to Fruit and Their Regulation

“…SAIV (Konno et al, 1993) and two kinds of acid invertase (AIV) (Nakagawa et al, 1971) from tomato fruit, two kinds of SAIV from Japanese pear fruit (Hashizume et al, 2003) and BAIV and SAIV from apple fruit (Pan et al, 2005b) were purified. Full-length cDNA of SAIV was cloned from fruits such as tomato fruit (Ohyama et al, 1998). Lin 5, Lin 6, and Lin 7 of BAIV pallalogues in tomato (Godt and Roitsch, 1997), GIN1 and GIN2 of VAIV pallalogues in grape berries (Davis and Robinson, 1996), BAIV in papaya fruit (Zhou et al, 2003a) and PsSAIV1 and PsSAIV2 of VAIV pallalogues in Japanese pear fruit .…”

“…In transgenic tomato fruit introduced antisense BAIV gene, BAIV activity was effective for the sink activity of fruit (Ohyama et al, 1995(Ohyama et al, , 1998. SPS also influenced sink activity because over-expression of the SPS gene increased partitioning and unloading of photoassimilates into fruit (Laporte et al, 2001;Nguyen-Quoc et al, 1999).…”

Characterization of Fruit-Type Sucrose Synthase Gene Promoters Isolated from Tomato and Japanese Pear

“…Hosui) were kindly provided by Dr. S. Yamaki of Nagoya University. Tomato total RNA was isolated from Micro-Tom fruit at 35 to 45 days after anthesis (DAA) according to the method of Ohyama et al 11 . Primers used for 5'-RACE analysis of TOMSSF and PypSUS1 mRNAs were TSPRACE+200a (5'-AATTGCATCGAACTCAGCCAAAAG-3') and PyPSSRACE+400a (5'-CGGTCAATTCCTCCACACTTAG-3'), respectively.…”

“…Total RNA was extracted from transformed Micro-Tom fruit by the method of Ohyama et al 11 . Samples of total RNA (5 µg/lane) was denatured by treatment with formaldehyde and fractionated by agarose gel electrophoresis 15 .…”