Cloning ofXenopusTFIIS and Its Expression in Oocytes and Early Embryos

Kugawa, Fumihiko; Shinga, Jun; Imagawa, Masayoshi; Arae, Ken; Nagano, Michiyo; Shibata, Mikihito; Shiokawa, K.; Aoki, Masatada

doi:10.1006/bbrc.1996.0780

Cited by 8 publications

(9 citation statements)

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“…Z54216), and fission and baker's yeast (Nakanishi et al, 1992;Williams and Kane, 1996;Marshall et al, 1990;Plant et al, 1996;Kugawa et al, 1996;Labhart, 1997a;Hirashima et al, 1988;Xu et al, 1994;Chen et al, 1992), as well as from archaebacteria and viruses (Langer and Zillig, 1993;Yamada et al, 1992;Rodriguez et al, 1992). More distantly related proteins include the 30-kDa subunit of vaccinia virus RNA polymerase (Ahn et al, 1990), a 21-kDa protein encoded by a HeLa cell cDNA (Yeh and Shatkin, 1994), and elongin A (Aso et al, 1995).…”

Section: Introductionmentioning

confidence: 96%

Identification of Novel Genes Encoding Transcription Elongation Factor TFIIS (TCEA) in Vertebrates: Conservation of Three Distinct TFIIS Isoforms in Frog, Mouse, and Human

Labhart

Morgan

1998

Genomics

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Section: Introductionmentioning

confidence: 96%

Identification of Novel Genes Encoding Transcription Elongation Factor TFIIS (TCEA) in Vertebrates: Conservation of Three Distinct TFIIS Isoforms in Frog, Mouse, and Human

Labhart

Morgan

1998

Genomics

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“…In our previous studies, we discovered, using the Xenopus oocyte development system, that XGTFIIS mRNA expression oscillated during Xenopus oogenesis, 16) and by genomic cloning, we found that like other basic transcription factors, XGTFIIS has a unique TATA-less transcriptional start site; this is a characteristic feature of house-keeping genes. 17) These findings suggested that a unique transcription regulation mechanism could be involved in XGTFIIS gene expression.…”

Section: Resultsmentioning

confidence: 97%

“…We have studied TFIIS in Xenopus laevis, 16,17) because this animal is very useful for studying development, and because we observed an intriguing oscillation of the Xenopus TFIIS mRNA expression during oogenesis. 16) After we first cloned the Xenopus TFIIS cDNA, other researchers identified two additional types of TFIIS genes in Xenopus laevis, and the one we cloned turned out to be the general type TFIIS (TCEA1) of Xenopus laevis (which we refer to here as XGTFIIS).…”

Section: Introductionmentioning

confidence: 99%

“…16) After we first cloned the Xenopus TFIIS cDNA, other researchers identified two additional types of TFIIS genes in Xenopus laevis, and the one we cloned turned out to be the general type TFIIS (TCEA1) of Xenopus laevis (which we refer to here as XGTFIIS). 18) In our previous genomic cloning of the XGTFIIS gene, 17) we found that XGTFIIS has two unique transcription start sites.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of the Promoter Region of the Xenopus General Type TFIIS (TCEA1) Gene

Kugawa

Aoki

2004

JOURNAL OF HEALTH SCIENCE

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TFIIS is a general transcription elongation factor that interacts with Elongin (SIII), a product of the tumor suppressor gene for the human hereditary disorder von Hippel-Lindau (VHL) disease. Here, we have studied the promoter region of the Xenopus general type TFIIS (XGTFIIS) gene using a luciferase assay. The previously cloned region 1600-bp upstream of the XGTFIIS gene was divided into 12 sequential deletion fragments, and each fragment was inserted into the pGL3-Basic vector, which was used as a reporter for a luciferase assay in the Xenopus cultured cell-line, XTC. Except for the proximal promoter region close to the transcription start sites, no positively or negatively regulating element was found for TFIIS gene expression within the 1600-bp upstream region of the XGTFIIS gene. Of the two previously identified transcription start sites, the one that is distal to the ATG codon was preferred over the proximal one.

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“…Previously, we cloned a Xenopus TFIIS cDNA and investigated its expression during oogenesis and early embryogenesis (Kugawa et al, 1996). We observed the oscillation of Xenopus TFIIS mRNA expression during oogenesis, and in a more pronounced manner during embryogenesis.…”

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confidence: 95%

Genomic Cloning of Xenopus TFIIS (TCEA1) and Identification of Its Transcription Start Site

Kugawa

Aoki

2002

DNA Sequence

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We previously cloned the cDNA of the transcription factor TFIIS (SII) from Xenopus laevis and showed that its expression was not constant during Xenopus development. To investigate its regulation, we cloned the genomic DNA of Xenopus TFIIS, focusing on the 5'-promoter region. Here, we present the Xenopus TFIIS genomic sequence (-1730 to +214) and transcription start site (cap site). We define the position of the primary cap site as the adenine located 142 bp upstream from the adenine of the ATG (Met) codon. Another putative start-site region, where 13 transcriptional start sites are clustered within 12 bp, was mapped about 100 bp downstream of the primary cap site. Although a computer search found putative trans-element binding sites proximal to two Xenopus TFIIS transcription start sites, we could not identify typical "TATA" or "CAATT" boxes upstream of the primary cap site, probably owing to TFIIS's character as a "house keeping gene".

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Cloning ofXenopusTFIIS and Its Expression in Oocytes and Early Embryos

Cited by 8 publications

References 0 publications

Identification of Novel Genes Encoding Transcription Elongation Factor TFIIS (TCEA) in Vertebrates: Conservation of Three Distinct TFIIS Isoforms in Frog, Mouse, and Human

Identification of Novel Genes Encoding Transcription Elongation Factor TFIIS (TCEA) in Vertebrates: Conservation of Three Distinct TFIIS Isoforms in Frog, Mouse, and Human

Analysis of the Promoter Region of the Xenopus General Type TFIIS (TCEA1) Gene

Genomic Cloning of Xenopus TFIIS (TCEA1) and Identification of Its Transcription Start Site

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