Cloning, Phylogenetic Analysis, and Distribution of Free Fatty Acid Receptor GPR120 Expression along the Gastrointestinal Tract of Housing versus Grazing Kid Goats

Abstract

G-protein-coupled receptor 120 (GPR120) is reported as a long-chain fatty acid (LCFA) receptor that elicits free fatty acid (FFA) regulation on metabolism homeostasis. The study aimed to clone the gpr120 gene of goats (g-GPR120) and subsequently investigate phylogenetic analysis and tissue distribution throughout the digestive tracts of kid goats, as well as the effect of housing versus grazing (H vs G) feeding systems on GPR120 expression. Partial coding sequence (CDS) of g-GPR120 was cloned and submitted to NCBI (accession no. KU161270 ). Phylogenetic analysis revealed that g-GPR120 shared higher homology in both mRNA and amino acid sequences for ruminants than nonruminants. Immunochemistry, real-time PCR, and Western blot analysis showed that g-GPR120 was expressed throughout the digestive tracts of goats. The expression of g-GPR120 was affected by feeding system and age, with greater expression of g-GPR120 in the G group. It was concluded that the g-GPR120-mediated LCFA chemosensing mechanism is widely present in the tongue and gastrointestinal tract of goats and that its expression can be affected by feeding system and age.

Characterization of Free Fatty Acid Receptor 4 and Its Involvement in Nutritional Control and Immune Response in Pacific Oysters (Crassostrea gigas)

“… 47 It is also expressed throughout the digestive tract of the goats. 48 Decreased activities of digestive enzymes (such as amylase, lipase, and pepsin) have previously been observed in mollusks upon starvation treatment. 49 − 51 Since then, this treatment has been applied to the study of digestive function in marine mollusks.…”

Multi-Omics Analysis of Mammary Metabolic Changes in Dairy Cows Exposed to Hypoxia

“…After treatment of hypoxia for 72 h, the level of genes related to milk fat synthesis were determined as described previously (28). In brief, 1 × 10 6 ∼1 × 10 7 cells were taken from each sample, and the cells were washed with PBS.…”

Comparison of the Effects of Nonprotein and Protein Nitrogen on Apoptosis and Autophagy of Rumen Epithelial Cells in Goats

“…After treatment for 36 h with 5 mM Met or NH 4 Cl, total RNA was extracted using AccuZol Total RNA Extraction Reagent (Bioneer, Daejeon, Korea) on the basis of the manufacturer's instructions [19]. Then, DNaseI (Thermo Scientific, Waltham, MA, USA) was used to eliminate genomic DNA, and a Nano Drop 2000 (Thermo Scientific, Waltham, MA, USA) was used to evaluate the RNA quality and quantity.…”