Cloning the root-specific Asy promoter and genes encoding chitinase 42 kDa of Trichoderma asperellum into the plant expression vector

Tue, Nguyen Hoang; Tuong, Tran Gia Cat; Trang, Pham Thi Huyen; Chung, Nguyễn Đức; Hoa, hung Thi Bich; Tien, Nguyen Quang Duc; Lộc, Nguyễn Hoàng

doi:10.7324/jabb.2022.100302

Cited by 7 publications

(4 citation statements)

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“…The downregulation of the miR1432- OsACOT module via STTM1432 resulted in a superior phenotype, including higher yield and disease resistance, when compared to the constitutive overexpression of OsACOT that only enhanced the yield with an unchanged blast disease resistance [ 163 ]. Many promoter sequences can be explored for tissue specificity, such as seed or fruit, flower, tuber, anther, root, and leaf, which can be utilized to express STTM in a particular organ [ 162 , 164 , 165 , 166 , 167 , 168 ].…”

Section: Application Of Sttm As a Constitutive And Spatial–temporal M...mentioning

confidence: 99%

Overview of Repressive miRNA Regulation by Short Tandem Target Mimic (STTM): Applications and Impact on Plant Biology

Othman

Mustaffa

Che-Othman

et al. 2023

Plants

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The application of miRNA mimic technology for silencing mature miRNA began in 2007. This technique originated from the discovery of the INDUCED BY PHOSPHATE STARVATION 1 (IPS1) gene, which was found to be a competitive mimic that prevents the cleavage of the targeted mRNA by miRNA inhibition at the post-transcriptional level. To date, various studies have been conducted to understand the molecular mimic mechanism and to improve the efficiency of this technology. As a result, several mimic tools have been developed: target mimicry (TM), short tandem target mimic (STTM), and molecular sponges (SPs). STTM is the most-developed tool due to its stability and effectiveness in decoying miRNA. This review discusses the application of STTM technology on the loss-of-function studies of miRNA and members from diverse plant species. A modified STTM approach for studying the function of miRNA with spatial–temporal expression under the control of specific promoters is further explored. STTM technology will enhance our understanding of the miRNA activity in plant-tissue-specific development and stress responses for applications in improving plant traits via miRNA regulation.

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Section: Application Of Sttm As a Constitutive And Spatial–temporal M...mentioning

confidence: 99%

Overview of Repressive miRNA Regulation by Short Tandem Target Mimic (STTM): Applications and Impact on Plant Biology

Othman

Mustaffa

Che-Othman

et al. 2023

Plants

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“…Agrobacterium tumefaciens strain LBA 4404 containing the pNHL20 vector harboring one of three chitinase genes (Chi42, syncodChi42-1, and syncodChi42-2) expressing 42 kDa chitinase was used in the present study (Tue et al 2021). Chi42 (HM191683.1) is a wild-type gene from T. asperellum SH16 (Loc et al 2011).…”

Section: Plant Expression Binary Vectormentioning

confidence: 99%

Enhancement of resistance against fungal pathogens in peanut (Arachis hypogaea L.) cultivar L14 by heterologous expression of gene encoding chitinase 42 kDa from Trichoderma asperellum SH16

Hoa

Tue

Trang

et al. 2022

Preprint

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This study reports the expression of the 42 kDa chitinase genes from Trichoderma asperellum SH16 in peanut (Arachis hypogaea) roots under the regulation of tissue-specific Asy promoter through Agrobacterium tumefaciens-mediated transformation. The 42 kDa chitinase genes, including one wild-type sequence (Chi42) and two synthetic sequences (syncodChi42-1 and syncodChi42-2) which were optimized for codon usage for plant expression, were incorporated into the peanut genome and successfully expressed in their roots. The investigation revealed that chitinase from two synthetic genes had higher activity than that from the wild-type gene, about 901 U/mg (140 U/mL) and 1124 U/mg (197 U/mL) vs about 739 U/mg (105 U/mL), respectively. Transgenic peanut roots also exhibited extracellular chitinase activity which was driven by signal peptide of rice amylase 3D gene against the pathogenic fungus Sclerotium rolfsii under in vitro conditions. The higher chitinase activity of two synthetic genes in peanut roots promises potential applications in the field of transgenic crops against phytopathogenic fungi.

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“…Nine chitinase transgenic peanut lines were used as test materials in the present study. Peanut varieties having the plant expression vector pMYV719 harboring three genes (Chi42, syncodChi42-1, and syncodChi42-2) expressing i42 kDa chitinase were used in this study I (Loc et al 2022).…”

Section: Plant Materialsmentioning

confidence: 99%

“…By using these technologies, the different origins derived chitinase genes have been successfully transformed into different types of plants such as rice (Lin et al 1995), tobacco (Zhu et al 1994), cucumber (Kishimotoiet al 2002, Italian ryegrass I (Takahashi et al 2005), banana (Sreeramanan et al 2009), cotton (Ganesan et al 2009), and peanut I (Chu et al 2008(Chu et al , 2013 and developed the ability of fungal resistance in these crops. Though various attempts were made to enhance the fungal resistance in groundnut by utilizing tobacco chitinase (Rohini and Rao 2001), barley oxalate oxidase (Livingstone et al 2005), mustard of defensin (Anuradha et al 2008) and β-1,3-glucanase from tobacco (Sundaresha et al 2009), there are currently no reports regarding the usage of 42 kDa chitinase genes from Trichoderma asperellum SH16, except for those published by Loc et al (2022), Hoa et al (2022a and Tue et al (2022). The antifungal activity of peanutcontaining 42 kDa chitinase genes (Hoa et al 2022b) and two genes (syncod Chi42-1 and syncod Chi42-2) were codons optimized for expression in the plant from the Chi42 gene (Luong et al 2021) were reported in these two types of research.…”

Section: Introductionmentioning

confidence: 99%

Growth and development of transgenic peanut (Arachis hypogaea) lines containing chitinase 42 kDa gene from Trichoderma asperellum SH16

Hoa¹,

Phuöng²,

Trang³

et al. 2022

J Exp Bio & Ag Sci

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Peanut (Arachis hypogaea L.) is vulnerable to many diseases. Vietnam and other regions where peanut is widely cultivated have a high threat of fungal and other plant diseases. Various fungicides are available to control the fungal disease but these have various harmful effects on the natural flora, fauna, and environment. Transgenic peanut lines which possess antifungal activity provide a possible solution in managing fungal diseases apart from the traditional resistance and fungicide usage. Therefore, this study evaluated the probable growth and development of chitinase transgenic peanut lines against Sclerotium rolfsii, a pathogen that causes “southern blight” in plants, under greenhouse conditions. This study provided evidence that through Agrobacterium itumefaciens mediated transformation, 42 kDa chitinase genes from Trichoderma asperellum, which is under the regulation of 35S promoter, were successfully incorporated into the peanut’s (A. hypogaea L.) genome and expressed in their plants. This evidence also demonstrated that transgenic peanut lines were suitable for growing and developing in the greenhouse. Further, it was reported that transgenic peanut lines took approximately 133 to 145 days from planting to maturity. These results also revealed that various growth characteristics of transgenic peanut lines having two synthetic genes (syncod Chi42-2 i.e. S2-2, S2-4, S2-6, and syncod Chi42-1 i.e. S1-1, S1-2, S1-3) were greater than that from the wild-type Chi42 (WT-1, WT-2, and WT-3). In addition, yield-related parameters including the number of mature pods, 100 pods weight and 100 seeds weight for all the transgenic peanut lines were higher than that of the non-transformed plant. Among the transgenic lines, line S2-4 exhibited significantly higher growth and yield than the other transgenic lines. These results demonstrated that 42 kDa chitinase genes overexpressing peanut lines could be a candidate for improvement against plants to phytopathogenic fungus S. rolfsii and high yield.

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Cloning the root-specific Asy promoter and genes encoding chitinase 42 kDa of Trichoderma asperellum into the plant expression vector

Cited by 7 publications

References 0 publications

Overview of Repressive miRNA Regulation by Short Tandem Target Mimic (STTM): Applications and Impact on Plant Biology

Overview of Repressive miRNA Regulation by Short Tandem Target Mimic (STTM): Applications and Impact on Plant Biology

Enhancement of resistance against fungal pathogens in peanut (Arachis hypogaea L.) cultivar L14 by heterologous expression of gene encoding chitinase 42 kDa from Trichoderma asperellum SH16

Growth and development of transgenic peanut (Arachis hypogaea) lines containing chitinase 42 kDa gene from Trichoderma asperellum SH16

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