Clostridium perfringens in suckling piglets with diarrhoea and its PCR typing and prevalence in the Czech Republic in 2001-2003

Brno, Czanderlova, L.,Sevaron Consulting Ltd.,; Hložek, Pavel; Chmelař, Dittmar; Lány, P.

doi:10.17221/5578-vetmed

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…The β2-toxin is being associated mainly with type A but has also been identified in association with types C and E in piglets and calves, respectively [32]. Similar findings by other investigators have also indicated that none of the isolates studied was positive to specific sequences of the gene coding the enterotoxin production (cpe) [33,34]. The enterotoxin (cpe) plays an important role in the development of intestinal disease in many animal species including man [7,35].…”

supporting

confidence: 77%

Genotyping of Clostridium perfringens Isolates from Domestic Livestock in Saudi Arabia

Omer

Al-Olayan

Babiker

et al. 2020

BioMed Research International

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The present study was undertaken to confirm the genetic identity of Clostridium perfringens isolates from domestic livestock in Saudi Arabia and to characterize the genes encoding to alpha, beta, epsilon, and iota (α-, β-, ε-, and ι-) toxins. C. perfringens were confirmed in 104 out of 136 isolates on multiplex polymerase chain reaction using specific primers amplifying genes related to toxins produced by C. perfringens. Genes encoding α-toxins were detected in 104 samples. Of the isolates, 80.8% were diagnosed as type A, 15.4% as type D, 2.9% as type C, and 0.96% as type B. None of the isolates has genes encoding iota (ι-) toxin. All isolates investigated yielded enterotoxin (cpe) products and none yielded β2 (cpb2-toxin) or NetB products. PLC gene sequences encoding α-toxin showed >96.7% similarity. Isolates which had α-toxins as well as enterotoxin (cpe) are regarded as type F. Phylogenetic analysis using maximum likelihood analysis yielded two clades, and the majority of the isolates were in one group while only two isolates clustered on the second clade. Within the Kingdom of Saudi Arabia strains, 54 variable positions and 23 polymorphic amino acids were noticed. Isolates with ε- and β-toxins were variable and were found to be close to those published for C. perfringens. ETX gene sequences encoding ε-toxins were found to be related to CPE sequences.

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confidence: 77%

Genotyping of Clostridium perfringens Isolates from Domestic Livestock in Saudi Arabia

Omer

Al-Olayan

Babiker

et al. 2020

BioMed Research International

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“…Attaching special attention to the development mechanism of severe course of the Perfringens disease it can be stated that it is endowed with high morbidity and mortality. According to the authors, in the pig-breeding farms of Hungary massive losses of piglets occurred due to Perfringens, which is followed by annual outbreaks for a long period (Altukhov and Dushenin, 1987;Urguev, 1987;Kudryashov, 1990;Satio, 1990;Czanderlova, et al, 2006;Salimov, 2006;Kudryashov and Svyatkovskiy, 2007;Sargsyan, 2012;Sidorchuk and Masimov, 2024). Complications of enteritis in newborn piglets can be caused by opportunistic bacteria, viruses and endoparasites belonging to the genera Escherichia coli and Salmonella.…”

Section: A R T I C L E I N F Omentioning

confidence: 99%

Epidemiological Analysis of the Swine Perfringens Disease in the Aragatsotn Region

Sargsyan

2024

AgriScience and Technology

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Over the recent 20 years, for the first time, the disease of swine perfringens has been studied in the Aragatsotn region of RA and related epidemiological analysis has been conducted. To identify A, C, and D serological types of Cl. Perfringens and special diagnostic sera produced by the Kursk biofactory were used. It has been found that the main serological infection type of swine perfringens is C and sometimes A and D subtypes. In the pig breeding farms of the mentioned region about 6887 heads of swine (aging from 3 days to 1 year old) were registered. Per the research results, about 45 out of 72 communities were recognized as insecure/vulnerable. As to the above-stated animal number, it should be mentioned, that 1722 heads of swine fell down, whereas 5510 were considered bacteria carriers. Thus, as a result of epidemiological analysis, the morbidity (bacteria carrying rate), mortality, insecurity/vulnerability, and focality coefficients (indicators) have made 0.80 (80 %), 0.25 (25 %), 0.63 (63 %), and 122 animals, respectively.

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Diagnosis of acute diarrhea in pigs and piglets in Meghalaya, India

Das¹,

Mazumder²,

Dutta³

et al. 2009

MJM

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This paper reports the diagnosis of acute diarrhoea in three male pigs and two female piglets died in an organized pig farm in Meghalaya, India. Intestinal contents and liver samples from dead pigs and piglets were investigated for isolation and identification of bacteria. Bacterial isolation confirmed the causative agent as Clostrodium perfringens. A total of eight C. perfringens were isolated out of 10 clinical samples. Isolates harbored identical double plasmid DNA of molecular weights 42.8 kb and 51.9 kb common to all. In polymerase chain reaction (PCR), all the eight (100%) clinical isolates harboured alpha toxin gene (cpa) and beta2 toxin gene (cpb2). None of the isolates were positive for beta, epsilon, iota and enterotoxin genes. The PCR analysis revealed that all isolates derived from acute diarrhoea belonged to C. perfringens type A. The partial cpa gene sequences from two Indian field isolates in BLAST showed similarity values greater than 99% to the published database sequences of C. perfringens. The phylogenetic analysis also revealed 98.6 to 100% sequence homology among the isolates studied. The findings suggested that C. perfringens type A having additional cpb2 gene might play a significant role in occurrence of acute diarrhoea in pigs and piglets in Meghalaya, India.

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Clostridium perfringens in suckling piglets with diarrhoea and its PCR typing and prevalence in the Czech Republic in 2001-2003

Cited by 3 publications

References 13 publications

Genotyping of Clostridium perfringens Isolates from Domestic Livestock in Saudi Arabia

Genotyping of Clostridium perfringens Isolates from Domestic Livestock in Saudi Arabia

Epidemiological Analysis of the Swine Perfringens Disease in the Aragatsotn Region

Diagnosis of acute diarrhea in pigs and piglets in Meghalaya, India

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