Clostridium viride sp. nov., a strictly anaerobic bacterium using 5-aminovalerate as growth substrate, previously assigned to Clostridium aminovalericum

Janssen, Peter H.; Schuhmann, Alexandra; Eikmanns, Ulrich; Messner, Paul; Sleytr, Uwe B.; Liesack, Werner

doi:10.1007/bf00282102

Cited by 22 publications

(7 citation statements)

References 32 publications

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“…1 lanes B, D, F and H), indicating that these species did not produce detectable caproic acid. Species of C. kluyveri, C. aminovalericum, C. celerecrescens and C. tepidiprofundi were only detected in ES enrichments, and confirmed their (except for C. kluyveri) inability or weak ability to produce caproic acid (Buckel et al, 1994;Slobodkina et al, 2008;Wang et al, 2014;Weimer and Stevenson, 2012). In conclusion, the PCR-DGGE approach confirmed that C. kluyveri was the main caproic acid-producing clostridia in all three distilleries.…”

Section: Direct Identification Of the Caproic Acid-producing Clostridsupporting

confidence: 56%

Identification and quantification of the caproic acid-producing bacterium Clostridium kluyveri in the fermentation of pit mud used for Chinese strong-aroma type liquor production

2015

International Journal of Food Microbiology

113

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Section: Direct Identification Of the Caproic Acid-producing Clostridsupporting

confidence: 56%

Identification and quantification of the caproic acid-producing bacterium Clostridium kluyveri in the fermentation of pit mud used for Chinese strong-aroma type liquor production

2015

International Journal of Food Microbiology

113

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“…A. hydrophila was the closest cultured species to retrieved Aeromonadaceae-related sequences and is a facultative aerobe that can ferment sugars (including glucose) to ethanol, formate, acetate, succinate, CO 2 and H 2 (Martin-Carnahan and Joseph, 2005a). Certain sequences of 'heavy fractions' from [ 13 C]-glucose treatments were closely affiliated with C. viride (Ruminococcaceae), an obligate anaerobe that can ferment glucose to acetate, formate, succinate, lactate and ethanol (Ezaki, 1984;Buckel et al, 1994). C. viride is …”

Section: Discussionmentioning

confidence: 99%

“…Methanogenic food web in the gut contents of Eudrilus K Schulz et al likewise able to ferment amino acids (Buckel et al, 1994), suggesting that bacteria related to C. viride might have additionally used endogenous amino acids in the earthworm gut contents (Drake and Horn, 2007). The formation of butyrate, isobutyrate and propionate (Figure 3) is indicative of the fermentation of amino acids (Nanninga and Gottschal, 1985;Gottschalk, 1986), and the high relative abundance of Peptostreptococcaceaeaffiliated sequences suggests that members of this amino-acid-fermenting taxon (for example, Clostridium lituseburense) may have utilized amino acids (Hippe et al, 1992;Ezaki, 2009).…”

Section: Discussionmentioning

confidence: 99%

Methanogenic food web in the gut contents of methane-emitting earthwormEudrilus eugeniaefrom Brazil

et al. 2015

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The anoxic saccharide-rich conditions of the earthworm gut provide an ideal transient habitat for ingested microbes capable of anaerobiosis. It was recently discovered that the earthworm Eudrilus eugeniae from Brazil can emit methane (CH 4 ) and that ingested methanogens might be associated with this emission. The objective of this study was to resolve trophic interactions of bacteria and methanogens in the methanogenic food web in the gut contents of E. eugeniae. RNA-based stable isotope probing of bacterial 16S rRNA as well as mcrA and mrtA (the alpha subunit of methyl-CoM reductase and its isoenzyme, respectively) of methanogens was performed with [ 13 C]-glucose as a model saccharide in the gut contents. Concomitant fermentations were augmented by the rapid consumption of glucose, yielding numerous products, including molecular hydrogen (H 2 ), carbon dioxide (CO 2 ), formate, acetate, ethanol, lactate, succinate and propionate. Aeromonadaceaeaffiliated facultative aerobes, and obligate anaerobes affiliated to Lachnospiraceae, Veillonellaceae and Ruminococcaceae were associated with the diverse fermentations. Methanogenesis was ongoing during incubations, and 13 C-labeling of CH 4 verified that supplemental [ 13 C]-glucose derived carbon was dissimilated to CH 4 . Hydrogenotrophic methanogens affiliated with Methanobacteriaceae and Methanoregulaceae were linked to methanogenesis, and acetogens related to Peptostreptoccocaceae were likewise found to be participants in the methanogenic food web. H 2 rather than acetate stimulated methanogenesis in the methanogenic gut content enrichments, and acetogens appeared to dissimilate supplemental H 2 to acetate in methanogenic enrichments. These findings provide insight on the processes and associated taxa potentially linked to methanogenesis and the turnover of organic carbon in the alimentary canal of methane-emitting E. eugeniae.

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“…Valerate is a fermentation product of 5-aminovalerate, e.g. by Clostridium viride [35], and 5-aminovalerate is a common product of anaerobic degradation of proline, arginine and ornithine [33]. Degradation of 5-aminovalerate is not sensitive to high H 2 partial pressures, but degradation of valerate is endergonic under standard conditions (valerate 3 +2 H 2 OCpropionate 3 + acetate 3 +H +2 H 2 ; vG³P = +48.3 kJ), thus explaining why it accumulated in the presence of CHCl 3 .…”

Section: Discussionmentioning

confidence: 99%

Selective inhibition of reactions involved in methanogenesis and fatty acid production on rice roots

Conrad

Klose

2000

FEMS Microbiology Ecology

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Washed excised roots of rice (Oryza sativa) produced H(2), CH(4) and fatty acids (millimolar concentrations of acetate, propionate, butyrate; micromolar concentrations of isovalerate, valerate) when incubated under anoxic conditions. Surface sterilization of the root material resulted in the inactivation of the production of CH(4), a strong reduction of the production of fatty acids and a transient (75 h) but complete inhibition of the production of H(2). Radioactive bicarbonate was incorporated into CH(4), acetate, propionate and butyrate. About 20-40% of the fatty acid carbon originated from CO(2) reduction. In the presence of phosphate, CH(4) was exclusively produced from H(2)/CO(2), since phosphate selectively inhibited acetoclastic methanogenesis. Acetoclastic methanogenesis was also selectively inhibited by methyl fluoride, while chloroform or 2-bromoethane sulfonate inhibited CH(4) production completely. Production of CH(4), acetate, propionate and butyrate from H(2)/CO(2) was always exergonic with Gibbs free energies <-20 kJ mol(-1) product. Chloroform inhibited the production of acetate and the incorporation of radioactive CO(2) into acetate. Simultaneously, H(2) was no longer consumed and accumulated, indicating that acetate was produced from H(2)/CO(2). Chloroform also resulted in increased production of propionate and butyrate whose formation from CO(2) became more exergonic upon addition of chloroform. Nevertheless, the incorporation of radioactive CO(2) into propionate and butyrate was inhibited by chloroform. The accumulation of propionate and butyrate in the presence of chloroform probably occurred by fermentation of organic matter, rather than by reduction of acetate and CO(2). [U-(14)C]Glucose was indeed converted to acetate, propionate, butyrate, CO(2) and CH(4). Radioactive acetate, CO(2) and CH(4) were also products of the degradation of [U-(14)C]cellulose and [U-(14)C]xylose. Addition of chloroform and methyl fluoride did not affect the product spectrum of [U-(14)C]glucose degradation. The application of combinations of selective inhibitors may be useful to elucidate anaerobic metabolic pathways in mixed microbial cultures and natural microbial communities.

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Clostridium viride sp. nov., a strictly anaerobic bacterium using 5-aminovalerate as growth substrate, previously assigned to Clostridium aminovalericum

Cited by 22 publications

References 32 publications

Identification and quantification of the caproic acid-producing bacterium Clostridium kluyveri in the fermentation of pit mud used for Chinese strong-aroma type liquor production

Identification and quantification of the caproic acid-producing bacterium Clostridium kluyveri in the fermentation of pit mud used for Chinese strong-aroma type liquor production

Methanogenic food web in the gut contents of methane-emitting earthwormEudrilus eugeniaefrom Brazil

Selective inhibition of reactions involved in methanogenesis and fatty acid production on rice roots

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