Cloxacillin nanostructured formulation for the treatment of bovine keratoconjunctivitis

Fonseca, Mariana; Maia, Julia M.S.; Varago, F.C.; Gern, J. C.; Carvalho, Wanessa A.; Silva, S.R.; Mosqueira, Vanessa Carla Furtado; Brandão, Humberto M.; Guimarães, Alexandre Silva

doi:10.1016/j.vas.2020.100089

Cited by 6 publications

(5 citation statements)

References 16 publications

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“…bovoculi , and BHV-1, and to establish if any relationship existed between the detected pathogens and clinical manifestations of disease in cattle herds from Eastern Kazakhstan. Infectious bovine keratoconjunctivitis is present in cattle from many parts of the world, including Kazakhstan [ 1 ]. Although Mor.…”

Section: Discussionmentioning

confidence: 99%

“…Infectious bovine keratoconjunctivitis (IBK) is a contagious ocular disease of cattle characterized by ulcerative keratitis, corneal edema, and conjunctivitis. This disease is the most common eye condition of cattle [1] and occurs worldwide, including in Kazakhstan [2][3][4]. Although corneal ulcerations in cattle may heal with limited corneal scar formation, it is not uncommon for IBK to result in complete or partial loss of vision [5].…”

Section: Introductionmentioning

confidence: 99%

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Association of different microbes and pathogenic factors in cases of infectious bovine keratoconjunctivitis in cattle from Eastern Kazakhstan

Kuibagarov,

Abdullina,

Ryskeldina

et al. 2023

Vet World

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Background and Aim: Infectious bovine keratoconjunctivitis (IBK) causes a significant economic loss to cattle industries in many countries, including Kazakhstan. Although Moraxella bovis is recognized as an etiologic agent of IBK, other bacterial and viral agents have been suspected to play a role in the pathogenesis of this disease. This study aimed to evaluate samples collected from the eyes of IBK-affected cattle in Eastern Kazakhstan at different stages of IBK for the presence of Mor. bovis, Moraxella bovoculi, Mycoplasma bovis, Mycoplasma bovoculi, and Bovine Herpes Virus Type 1 (BHV-1) and to characterize Mor. bovoculi pilA gene sequence diversity from Mor. bovoculi positive samples. Materials and Methods: Individual ocular swabs (n = 168) were collected from cattle that had clinical signs of IBK during the summer of 2022 on farms in the Abay region of Kazakhstan. Eye lesion scores (1, 2, and 3) were assigned depending on the degree of ocular damage. Infectious bovine keratoconjunctivitis-associated organisms were detected using a multiplex real-time polymerase chain reaction assay. The Mor. bovoculi pilA gene was sequenced from Mor. bovoculi positive samples. Results: Mycoplasma bovis and BHV-1 were not detected in any of the collected samples. Mycoplasma bovoculi was identified in the majority of samples overall, usually in mixed infection with Moraxella spp. Moraxella bovoculi was detected in 76.2% of animals and predominated in animals with eye lesion scores 2 and 3. Mycoplasma bovoculi was detected only in association with Mor. bovis and/or Mor. bovoculi in animals with eye lesion scores 2 and 3. Moraxella bovis was found in 57.7% of animals and was always identified in association with another organism. Sequencing of the pilA gene in 96 samples from Mor. bovoculi positive samples identified five PilA groups. The majority belonged to PilA group A. However, three new PilA groups were identified and designated PilA groups N, O, and P. Conclusion: The results indicate a high prevalence of Myc. bovoculi and Mor. bovoculi in eyes of cattle with IBK on livestock farms in Eastern Kazakhstan. Additional novel Mor. bovoculi PilA groups were identified. Keywords: bovine herpes virus, infectious bovine keratoconjunctivitis, Moraxella bovis, Moraxella bovoculi, multiplex real-time polymerase chain reaction, Mycoplasma bovis, Mycoplasma bovoculi.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Association of different microbes and pathogenic factors in cases of infectious bovine keratoconjunctivitis in cattle from Eastern Kazakhstan

Kuibagarov,

Abdullina,

Ryskeldina

et al. 2023

Vet World

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“…The use of polymeric nanocarriers promotes sustained drug release in the ocular mucosa, maintains a high concentration of nanostructured antimicrobial agents, reduces the number of doses, and minimizes the antibiotic residues in mammalian milk. In a study by Fonseca et al, PCL-coated CS nanocapsules containing cloxacillin were developed to treat infectious bovine keratoconjunctivitis (IBK) caused by Moraxella bovis [ 99 ]. This was the first study in Brazil using nanoantibiotics for the treatment of clinical IBK.…”

Section: Polymeric Nps For the Delivery Of Veterinary Antimicrobial M...mentioning

confidence: 99%

An Overview of Polymeric Nanoplatforms to Deliver Veterinary Antimicrobials

Zhou,

Guo,

Dai

et al. 2024

Nanomaterials

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There is an urgent need to find new solutions for the global dilemma of increasing antibiotic resistance in humans and animals. Modifying the performance of existing antibiotics using the nanocarrier drug delivery system (DDS) is a good option considering economic costs, labor costs, and time investment compared to the development of new antibiotics. Numerous studies on nanomedicine carriers that can be used for humans are available in the literature, but relatively few studies have been reported specifically for veterinary pharmaceutical products. Polymer-based nano-DDS are becoming a research hotspot in the pharmaceutical industry owing to their advantages, such as stability and modifiability. This review presents current research progress on polymer-based nanodelivery systems for veterinary antimicrobial drugs, focusing on the role of polymeric materials in enhancing drug performance. The use of polymer-based nanoformulations improves treatment compliance in livestock and companion animals, thereby reducing the workload of managers. Although promising advances have been made, many obstacles remain to be addressed before nanoformulations can be used in a clinical setting. Some crucial issues currently facing this field, including toxicity, quality control, and mass production, are discussed in this review. With the continuous optimization of nanotechnology, polymer-based DDS has shown its potential in reducing antibiotic resistance to veterinary medicines.

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“…nfectious Bovine Keratoconjunctivitis (IBK) has been associated with ocular disease characterized by lacrimation, photophobia, conjunctivitis, keratitis, corneal opacity, and corneal ulceration in cattle and, the disease causes severe economic loss in cattle breeding worldwide as a highly contagious disease (1)(2)(3)(4)(5)(6). Moraxella bovis (M.…”

Section: Introductionmentioning

confidence: 99%

“…bovis) is considered as primary etiologic agent of IBK (3,5,7,8) which was characterized as the Gramnegative aerobic/microaerophilic rod-shaped coccobacillus (9). Many serotypes of M. bovis vary in pathogenicity and some other Moraxella species like…”

Section: Introductionmentioning

confidence: 99%

Determination of seroprevalence of infectious bovine keratoconjuctivitis disease by ELISA and AGID

Ataseven¹

2020

Atatürk Üniversitesi Veteriner Bilimleri Dergisi

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Infectious Bovine Keratoconjunctivitis (IBK) is a highly contagious disease of conjunctiva and cornea in cattle which causes significant economic losses in cattle breeding. The objective of this study is to determine serum antibodies against M. bovis and obtaining information about the seroprevalence of IBK by AGID test and ELISA. Serum samples, collected from 552 cattle in different herds in Turkey were examined for specific antibodies against Moraxella bovis (M. bovis). The seropositivity of IBK in cattle sera with clinical signs was determined as 26.1% and 5.8% by ELISA and AGID, respectively. Seropositivity in sera samples of healthy appeared cattle were detected as 26.3% and 5.6% by ELISA and AGID, respectively. According to the results, the highest seropositivity (36.5%) was detected in the animals aged 12-24 months. Similar seroprevalence detection rates were recorded in cattle with and without clinical signs. Clinical signs, spesifically, does not give knowledge about the exact percentage of the IBK. Therefore, besides isolation and identification of the agent, serological tests like ELISA, as a screening test, should be preferred to determine the presence and spread of IBK in the herd.

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Cloxacillin nanostructured formulation for the treatment of bovine keratoconjunctivitis

Cited by 6 publications

References 16 publications

Association of different microbes and pathogenic factors in cases of infectious bovine keratoconjunctivitis in cattle from Eastern Kazakhstan

Association of different microbes and pathogenic factors in cases of infectious bovine keratoconjunctivitis in cattle from Eastern Kazakhstan

An Overview of Polymeric Nanoplatforms to Deliver Veterinary Antimicrobials

Determination of seroprevalence of infectious bovine keratoconjuctivitis disease by ELISA and AGID

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