Co-circulation of Hepatitis E Virus (HEV) Genotype 3 and Moose-HEV-Like Strains in Free-Ranging-Spotted Deer (Axis axis) in Uruguay

Cancela, Florencia; Cravino, Alexandra; Icasuriaga, Romina; González, Pablo; Bentancor, Federico; Leizagoyen, Carmen; Echaides, César; Ferreiro, Irene; Cabrera, Andrés; Arbiza, Juan; Mirazo, Santiago

doi:10.1007/s12560-023-09563-2

Cited by 2 publications

(2 citation statements)

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“…HEV infection has also been identified and characterized in domestic pigs, with an antibody and RNA prevalence of 46.8% and 16.6%, respectively, and in wild boars, indicating a widespread circulation of the virus in their natural reservoirs [ 16 ]. Additionally, we showed that HEV-3 could also infect and be transmitted among white-collared peccaries (New World pigs) and spotted deer ( Axis axis ) [ 17 , 40 ]. In this work, to provide novel insights into the molecular epidemiology and transmission patterns of HEV in Uruguay we reexamined the phylogenetic relationships with updated data and additional analyses.…”

Section: Discussionmentioning

confidence: 99%

“…Unfortunately, ORF2 sequences from white-collared peccaries and spotted deer were not available for this study and this is a limitation in terms of exploring potential transmission paths involving other ecological reservoirs/hosts. However, previous analyses carried out with ORF1 partial sequences had shown close phylogenetic relationships with sequences of human origin [ 17 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

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Epidemiology Update of Hepatitis E Virus (HEV) in Uruguay: Subtyping, Environmental Surveillance and Zoonotic Transmission

Cancela,

Icasuriaga,

Cuevas

et al. 2023

Viruses

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Hepatitis E Virus (HEV) infection is an emergent zoonotic disease of increasing concern in developed regions. HEV genotype 3 (HEV-3) is mainly transmitted through consumption of contaminated food in high-income countries and is classified into at least 13 subtypes (3a–3n), based on p-distance values from complete genomes. In Latin America, HEV epidemiology studies are very scant. Our group has previously detected HEV3 in clinical cases, swine, wild boars, captive white-collared peccaries, and spotted deer from Uruguay. Herein, we aimed to provide novel insights and an updated overview of the molecular epidemiology of zoonotic HEV in Uruguay, including data from wastewater-based surveillance studies. A thorough analysis of HEV whole genomes and partial ORF2 sequences from Uruguayan human and domestic pig strains showed that they formed a separate monophyletic cluster with high nucleotide identity and exhibited p-distance values over the established cut-off (0.093) compared with reference subtypes’ sequences. Furthermore, we found an overall prevalence of 10.87% (10/92) in wastewater, where two samples revealed a close relationship with humans, and animal reservoirs/hosts isolates from Uruguay. In conclusion, a single, new HEV-3 subtype currently circulates in different epidemiological settings in Uruguay, and we propose its designation as 3o along with its reference sequence.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Epidemiology Update of Hepatitis E Virus (HEV) in Uruguay: Subtyping, Environmental Surveillance and Zoonotic Transmission

Cancela,

Icasuriaga,

Cuevas

et al. 2023

Viruses

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Comprehensive analysis of mRNA Transcriptome Profiles in HepG2 Cells Expressing Genotype IV Swine Hepatitis E Virus ORF3

Jiao,

Meng,

Jiao

et al. 2024

Preprint

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Background Porcine hepatitis E is a zoonotic infectious disease caused by swine hepatitis E virus (HEV), open reading frames 3 is an important virulence protein of porcine HEV, which plays an important role in the release of viral particles and host innate immune response, regulation of autophagy and apoptosis, etc., but its main function and pathogenic mechanism are not perfect in current research. Results In our study, adenoviruses ADV4-ORF3 and ADV4-GFP were successfully constructed and mediated the overexpression of enhanced green fluorescent protein (EGFP)-ORF3 and EGFP in HepG2 cells. A total of 217 differentially expressed messenger RNAs (mRNAs) were screened by high-throughput sequencing, and 27 statistically significant differentially expressed genes were screened for further quantitative real-time reverse transcription (qRT-PCR) verification by functional enrichment (Gene Ontology [GO] and Kyoto Encyclopedia of Genes and Genomes [KEGG]). They are mainly involved in 6 pathways: cellular response to unfolded protein, Inflammatory response, cytokine activity, TNF signaling pathway, Influenza A, and Pathways in cancer. Conclusions The differential genes were successfully verified, which laid a genetic foundation for the physiological function and mechanism of HEV ORF3

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Co-circulation of Hepatitis E Virus (HEV) Genotype 3 and Moose-HEV-Like Strains in Free-Ranging-Spotted Deer (Axis axis) in Uruguay

Cited by 2 publications

References 46 publications

Epidemiology Update of Hepatitis E Virus (HEV) in Uruguay: Subtyping, Environmental Surveillance and Zoonotic Transmission

Epidemiology Update of Hepatitis E Virus (HEV) in Uruguay: Subtyping, Environmental Surveillance and Zoonotic Transmission

Comprehensive analysis of mRNA Transcriptome Profiles in HepG2 Cells Expressing Genotype IV Swine Hepatitis E Virus ORF3

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