Co–Culture with Amniotic Membrane Stem Cells for Oocyte Maturation and Early Embryonic Development in Bovine

Han, Kuang-Wei; Chun, Ju Lan; Lee, Ji Hey; Kim, Keun Jung; Lee, Kyung Bon; Gotoh, Taksfumi; Hee, Chang Hong; Kim, Min Kyu

doi:10.5109/1526400

Cited by 2 publications

(7 citation statements)

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“…They suggested that epidermal growth factor (EGF) produced by e‐AMSCs, which is normally secreted from the endometrium, may be responsible for this function (Lange‐Consiglio et al., 2012). However, the results of this study on co‐culture were inconsistent with the results of some studies (Han et al., 2015; Miranda et al., 2016; Opiela et al., 2018). The reasons for this discrepancy may be related to some variables such as embryonic stage in co‐culture, media composition, cell type, MSCs passage number and confluency of stem cell.…”

Section: Discussioncontrasting

confidence: 99%

“…Additionally, their differentiation into adipose and bone tissues was consistent with other studies (Díaz‐Prado et al., 2011; Lv et al., 2014). Considering the innate role of amniotic membrane in pregnancy, ease and non‐invasive isolation and faster growth rate of its stem cells compared to the bone marrow and adipose tissue derived MSCs, as well as its positive effect on in‐vitro oocyte maturation and embryonic development (Han et al., 2015), their use has been highly regarded by researchers. On the other hand, considering the sterility of the human amniotic membrane and its application in tissue repair and the possibility of its co‐culture with the human oocyte and embryo, it has attracted enormous interest.…”

Section: Discussionmentioning

confidence: 99%

“…It seems that hAMSCs due to its special characteristics (reproductive origin and its activity during pregnancy) compared to other stem cells (e.g. adipose‐derived stem cells) have a greater effect on embryo development (Han et al., 2015; Ling et al., 2008).…”

Section: Discussionmentioning

confidence: 99%

“…adipose-derived stem cells) have a greater effect on embryo development (Han et al, 2015;Ling et al, 2008).…”

Section: Ta B L Ementioning

confidence: 99%

“…Among different types of stem cells, oocyte co‐culture with amniotic membrane‐derived stem cells reduced the number of apoptotic cells in the resulting embryo by increasing glutathione and the expression of anti‐apoptotic genes, which was reflected by increased the rates of cleavage and blastocyst formation (Han et al., 2015). In addition, it has been shown that the monolayer of equine amniotic progenitor epithelial cells as feeding cells in bovine embryo culture medium increases blastocyst rate (Lange‐Consiglio et al., 2012).…”

Section: Introductionmentioning

confidence: 99%

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Human amniotic membrane stem cells’ conditioned medium has better support for in‐vitro production of bovine embryos than FBS

Shadmanesh

Nazari

Shirazi

et al. 2021

Reprod Domestic Animals

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Apart from oocyte quality, the media used has a significant effect on the production and quality of blastocysts produced in vitro. This study was designed to evaluate the replacement of serum with human amniotic membrane stem cells’ conditioned medium (hAMSCs‐CM) during bovine embryo culture on the quantity and quality of produced blastocysts. The in‐vitro‐produced embryos on the third day of IVC were randomly divided into the following culture groups: SOFaa + 5% FBS (Control), SOFaa + 5% hAMSCs‐CM (5% CM), SOFaa + 2.5% hAMSCs‐CM + 2.5% FBS (2.5% CM) and SOFaa + hAMSC co‐culture (co‐culture). The blastocyst and hatching rates, blastocyst cells number (the number of trophectoderm, inner cell mass and total cells), and the expression of some developmentally important genes (OCT4, PLAC8 and COX2 genes) in the treated groups, especially in the 5% CM, compared to the control had improved (p < .05). No significant difference was observed between groups for viability and hatching rate in vitrified‐warmed blastocysts. Due to the positive effect of hAMSCs’ conditioned medium (hAMSCs‐CM) on blastocyst production, as well as its ease of preparation and the need to avoid the transmission of microbial contamination to the culture medium, hAMSCs‐CM can be used as a suitable alternative to FBS during 3 to 8 days of bovine embryo culture.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…adipose-derived stem cells) have a greater effect on embryo development (Han et al, 2015;Ling et al, 2008).…”

Section: Ta B L Ementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Human amniotic membrane stem cells’ conditioned medium has better support for in‐vitro production of bovine embryos than FBS

Shadmanesh

Nazari

Shirazi

et al. 2021

Reprod Domestic Animals

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Scoping review of primary studies evaluating Bovine in vitro oocyte maturation and embryo development

Lizarraga,

Gaxiola,

Castro del Campo

et al. 2024

Vet Mex OA

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We conducted a scoping review to 1) map and categorize published studies reporting interventions during the in vitro production of Bovine embryos and 2) qualitatively summarize the effects of treatments based on hormones, growth factors, sera, and reproductive fluids during oocyte maturation and embryo development. We searched electronic databases using keywords ('Bovine', 'embryo', 'blastocyst', 'oocyte', 'in vitro', 'quality') derived from the PIOS approach of the PRISMA statement. We identified 231 studies published during 2000−2021, with 133 being published between 2012 and 2021. The 231 studies were classified into four treatment categories: culture conditions (28), medium composition (45), bioactive supplements (79), and other additives (79), with 19 subcategories within these categories. A total of 77 studies included hormones, growth factors, sera, and reproductive fluids, among which 53 studies reported a positive effect on embryo production. Hormone-based treatments using melatonin, gonadotropins, and steroids were the most effective, followed by interventions assessing growth factors such as EGF, FGF, IGF-1, and BMP. These treatments improved oocyte competence, cytoplasmic/nuclear maturation, oocyte quality, and blastocyst development. Although free-serum media and synthetic/alternative products can partially or totally replace serum, it is sometimes necessary even at low concentrations. At high concentrations, hormones or growth factors may have detrimental effects on oocyte nuclear maturation, impair embryo development, and decrease embryo survival. Further meta-analyses are needed to estimate the intervention-specific effects. Future research should focus on interventions that have a positive effect and can be used extensively for Bovine production.

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Co–Culture with Amniotic Membrane Stem Cells for Oocyte Maturation and Early Embryonic Development in Bovine

Cited by 2 publications

References 0 publications

Human amniotic membrane stem cells’ conditioned medium has better support for in‐vitro production of bovine embryos than FBS

Human amniotic membrane stem cells’ conditioned medium has better support for in‐vitro production of bovine embryos than FBS

Scoping review of primary studies evaluating Bovine in vitro oocyte maturation and embryo development

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