Co-culture with Tetragenococcus halophilus improved the ethanol tolerance of Zygosaccharomyces rouxii by maintaining cell surface properties

Yao, Shangjie; Hao, Liang; Zhou, Rongqing; Yao, Jin; Huang, Jun; Wu, Chongde

doi:10.1016/j.fm.2021.103750

Cited by 15 publications

(11 citation statements)

References 56 publications

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“…In addition, studies showed that Z. rouxii cells with better plasma membrane integrity and higher content of amino acids and organic acids had better viability under 120 g L −1 NaCl stress 40 . As observed by LSCM, SEM and AFM, the cell wall integrity, cell morphology and surface roughness of the cell membrane were improved after exogenous fatty acid addition, which may be due to the involvement of exogenous fatty acids in repairing the dents and wrinkles along the membrane surface to reduce the surface roughness 41,42 . These results can be well correlated with the enhancement of growth performance by the addition of exogenous fatty acids.…”

Section: Discussionmentioning

confidence: 66%

Metabolomics analysis of salt tolerance of Zygosaccharomyces rouxii and guided exogenous fatty acid addition for improved salt tolerance

Wang

Huang

et al. 2022

J Sci Food Agric

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BACKGROUND: Zygosaccharomyces rouxii plays an irreplaceable role in the manufacture of traditional fermented foods, which are produced in a high-salt environment. However, there is little research on strategies for improving salt tolerance of Z. rouxii.RESULTS: In this study, metabolomics was used to reveal the changes in intracellular metabolites under salt stress, and the results show that most of the carbohydrate contents decreased, the contents of xanthohumol and glycerol increased (fold change 4.07 and 5.35, respectively), while the contents of galactinol, xylitol and D-threitol decreased (fold change −9.43, −5.83 and −3.59, respectively). In addition, the content of four amino acids and six organic acids decreased, while that of the ten nucleotides increased. Notably, except for stearic acid (C18:0), all fatty acid contents increased. Guided by the metabolomics results, the effect of addition of seven exogenous fatty acids (C12:0, C14:0, C16:0, C18:0, C16:1, C18:1, and C18:2) on the salt tolerance of Z. rouxii was analyzed, and the results suggested that four exogenous fatty acids (C12:0, C16:0, C16:1, and C18:1) can increase the biomass yield and maximum growth rate. Physiological analyses demonstrated that exogenous fatty acids could regulate the distribution of fatty acids in the cell membrane, increase the degree of unsaturation, improve membrane fluidity, and maintain cell integrity, morphology and surface roughness.CONCLUSION: These results are applicable to revealing the metabolic mechanisms of Z. rouxii under salt stress and screening potential protective agents to improve stress resistance by adding exogenous fatty acids.

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Section: Discussionmentioning

confidence: 66%

Metabolomics analysis of salt tolerance of Zygosaccharomyces rouxii and guided exogenous fatty acid addition for improved salt tolerance

Wang

Huang

et al. 2022

J Sci Food Agric

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“…After removing saline solution, the same medium described above was added in plates gently. Planktonic and biofilm-dispersed cells were stressed at 30 • C and 100 rpm, while biofilm cells were stressed statically at 30 • C. Heat stress was performed in a 52 • C bath for 1.5 h. Acid stress and oxidative stress were performed for 1.5 h, ethanol stress for 3 h. The live cell count method and survival rate calculation were described as in our previous study (Yao et al, 2021).…”

Section: Cell Viability Assessment During Environment Stressesmentioning

confidence: 99%

Formation of Biofilm by Tetragenococcus halophilus Benefited Stress Tolerance and Anti-biofilm Activity Against S. aureus and S. Typhimurium

et al. 2022

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Tetragenococcus halophilus, a halophilic lactic acid bacterium (LAB), plays an important role in the production of high-salt fermented foods. Generally, formation of biofilm benefits the fitness of cells when faced with competitive and increasingly hostile fermented environments. In this work, the biofilm-forming capacity of T. halophilus was investigated. The results showed that the optimal conditions for biofilm formation by T. halophilus were at 3–9% salt content, 0–6% ethanol content, pH 7.0, 30°C, and on the surface of stainless steel. Confocal laser scanning microscopy (CLSM) analysis presented a dense and flat biofilm with a thickness of about 24 μm, and higher amounts of live cells were located near the surface of biofilm and more dead cells located at the bottom. Proteins, polysaccharides, extracellular-DNA (eDNA), and humic-like substances were all proved to take part in biofilm formation. Higher basic surface charge, greater hydrophilicity, and lower intracellular lactate dehydrogenase (LDH) activities were detected in T. halophilus grown in biofilms. Atomic force microscopy (AFM) imaging revealed that biofilm cultures of T. halophilus had stronger surface adhesion forces than planktonic cells. Cells in biofilm exhibited higher cell viability under acid stress, ethanol stress, heat stress, and oxidative stress. In addition, T. halophilus biofilms exhibited aggregation activity and anti-biofilm activity against Staphylococcus aureus and Salmonella Typhimurium. Results presented in the study may contribute to enhancing stress tolerance of T. halophilus and utilize their antagonistic activities against foodborne pathogens during the production of fermented foods.

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“…Total RNA extracted as described above was reverse transcribed into cDNA using the PrimeScript reagent Kit (TaKaRa Biotechnology, Liaoning, China). TB Green Premix Ex Taq Kit (TaKaRa Biotechnology, Liaoning, China) was used to quantitate target genes according to the method described by Yao et al 19 PCR reaction conditions were as follows: heating to 95 °C for 30 s, denaturation at 95 °C for 5 s, annealing at 55 °C for 1 min and extension at 72 °C for 30 s, for a total of 40 cycles. Primer sequences are listed in Supporting Information, Table S1.…”

Section: Quantitative Real-time Pcr (Rt-qpcr) Validation Of Rnaseq Re...mentioning

confidence: 99%

“…In order to investigate the potential molecular mechanism of Crypthecodinium cohnii mutants with high growth and lipid content, Liu et al 18 studied the changes in mutants using RNA-seq technology, and transcriptomic analysis indicated that the transcripts involved in fatty acid biosynthesis, energy and amino acid metabolism were upregulated in the mutant compared to the wild type. In addition, Yao et al 19 used RNA-seq to investigate the effects of the co-culture on resistance of Zygosaccharomyces rouxii to ethanol stress and studied the changes of gene transcript levels. Transcriptomic analysis indicated that the genes involved in the mitogenactivated protein kinase signaling pathway had higher transcript levels in co-culture cells.…”

Section: Introductionmentioning

confidence: 99%

“…studied the changes in mutants using RNA‐seq technology, and transcriptomic analysis indicated that the transcripts involved in fatty acid biosynthesis, energy and amino acid metabolism were upregulated in the mutant compared to the wild type. In addition, Yao et al 19 . used RNA‐seq to investigate the effects of the co‐culture on resistance of Zygosaccharomyces rouxii to ethanol stress and studied the changes of gene transcript levels.…”

Section: Introductionmentioning

confidence: 99%

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Molecular mechanism of high‐production tannase of Aspergillus carbonariusNCUF M8 after ARTP mutagenesis: revealed by RNA‐seq and molecular docking

et al. 2022

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BACKGROUND Tannase is an enzyme produced by microbial fermentation and is widely used in the food industry; however, the molecular mechanism of tannase production by Aspergillus has not yet been studied. This study was conducted to reveal the differences in Aspergillus carbonarius tannase enzymatic characterization, secondary structures and molecular mechanisms after treatment of the strain with atmospheric and room temperature plasma (ARTP). RESULTS The results showed that the specific activity of tannase was improved by ARTP treatment, and it showed higher thermostability and tolerance to metal ions and additives. The enzymatic characterization and molecular docking results indicated that tannase had a higher affinity and catalytic rate with tannic acid as a substrate after ARTP treatment. In addition, the docking results indicated that Aspergillus tannases may catalyze tannic acid by forming two hydrogen‐bonding networks with neighboring residues. RNA‐seq analysis indicated that changes in steroid biosynthesis, glutathione metabolism, glycerolipid metabolism, oxidative phosphorylation pathway and mitogen‐activated protein kinase signaling pathways might be crucial reasons for the high production of tannase. CONCLUSION ARTP enhanced the yield and properties of A. carbonarius tannase by changing the enzyme structure and cell metabolism. This study provides a theoretical basis for elucidating the molecular mechanism underlying high production of Aspergillus tannases. © 2022 Society of Chemical Industry.

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Co-culture with Tetragenococcus halophilus improved the ethanol tolerance of Zygosaccharomyces rouxii by maintaining cell surface properties

Cited by 15 publications

References 56 publications

Metabolomics analysis of salt tolerance of Zygosaccharomyces rouxii and guided exogenous fatty acid addition for improved salt tolerance

Metabolomics analysis of salt tolerance of Zygosaccharomyces rouxii and guided exogenous fatty acid addition for improved salt tolerance

Formation of Biofilm by Tetragenococcus halophilus Benefited Stress Tolerance and Anti-biofilm Activity Against S. aureus and S. Typhimurium

Molecular mechanism of high‐production tannase of Aspergillus carbonariusNCUF M8 after ARTP mutagenesis: revealed by RNA‐seq and molecular docking

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