Co-dependence of genotype and dietary protein intake to affect expression on amino acid/peptide transporters in porcine skeletal muscle

Liu, Y.; Kong, Xiangfeng; Li, F.; Tan, Bin; Li, Y.; Duan, Yehui; Yin, Yulong; He, Jun; Hu, Chengjun; Blachier, François; Wu, Guoyao

doi:10.1007/s00726-015-2066-2

Cited by 27 publications

(21 citation statements)

References 99 publications

(79 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After centrifugation at 12,000 × g for 10 min, 2 ml of the supernatant liquid was mixed with 2 ml of 8% 5‐sulphosalicylic acid. After centrifugation again at 12,000 × g for 10 min, the supernatant fluid was filtered through a 0.45 μm membrane before analysis of AAs using an ion‐exchange AA analyzer (L8800, Hitachi, Tokyo, Japan) (Liu et al, ).…”

Section: Methodsmentioning

confidence: 99%

Dietary mulberry leaf powder affects growth performance, carcass traits and meat quality in finishing pigs

Liu

Peng

et al. 2019

Animal Physiology Nutrition

View full text Add to dashboard Cite

This study was conducted to evaluate the effect of mulberry leaves as an alternative source of protein on growth performance, carcass traits and meat quality in finishing pigs. A total of 180 Xiangcun Black pigs were randomly assigned to five treatment groups with six pens of six pigs per pen. The pigs were provided with a basal diet or a diet contained 3%, 6%, 9% or 12% of mulberry leaf powder during a 50‐day experiment period. The results showed that dietary mulberry leaf powder had no negative effect on growth performance in Xiangcun Black pigs, except in the 12% mulberry group, where final body weight and average daily gain decreased (p < .05) and feed to gain ratio of the pigs increased (p < .05). Dietary mulberry inclusion decreased (quadratic, p < .05) the back fat thickness, fibre mean cross‐sectional area (CSA) in the longissimus dorsi (LD) muscle and mRNA expression levels of myosin heavy chain (MyHC) IIb in LD and biceps femoris (BF) muscles, while increased (linear or quadratic, p < .05) the plasma concentration of albumin, levels of crude protein (CP), inosine monophosphate (IMP) and several amino acids in muscle tissues. When compared with the other groups, the 9% mulberry diet increased (p < .05) loin‐eye area and contents of CP and IMP in muscles, while decreased (p < .05) plasma activity of cholinesterase and concentrations of uric acid and urea. The 6% mulberry diet had the lowest fibre mean CSA and shear force and increased total fibre number of the LD muscle, when compared with the other groups. These results suggest that including mulberry in the diet at <12% is an effective feed crop to improve meat quality and the chemical composition of muscle without negatively affecting growth performance.

show abstract

Section: Methodsmentioning

confidence: 99%

Dietary mulberry leaf powder affects growth performance, carcass traits and meat quality in finishing pigs

Liu

Peng

et al. 2019

Animal Physiology Nutrition

View full text Add to dashboard Cite

show abstract

“…The use of fermentable carbohydrates to reduce deleterious protein-derived bacterial metabolites in pigs is well established (140), and for instance, soybean oligosaccharides have been shown to increase the presumably beneficial short-chain fatty acids while decreasing the protein-derived catabolites in the intestinal luminal content in weaned piglets (141). Last, interesting data have been recently obtained regarding the impact of the amount of dietary protein consumed by pigs on parameters like expression of AA and peptide transporters (125), or signaling pathways related to protein synthesis in muscles (142). Then, from these examples, it appears that using pig models for confirmation of data obtained in rodents represents a useful experimental strategy before further development of clinical studies implying dietary intervention with human volunteers, notably in overweight and obese individuals.…”

Section: The Pig Model For Research On Obesitymentioning

confidence: 99%

Animal Models for the Study of the Relationships between Diet and Obesity: A Focus on Dietary Protein and Estrogen Deficiency

et al. 2017

View full text Add to dashboard Cite

Obesity is an increasing major public health concern asking for dietary strategies to limit weight gain and associated comorbidities. In this review, we present animal models, particularly rats and mice, which have been extensively used by scientists to understand the consequences of diet quality on weight gain and health. Notably, modulation of dietary protein quantity and/or quality has been shown to exert huge effects on body composition homeostasis through the modulation of food intake, energy expenditure, and metabolic pathways. Interestingly, the perinatal window appears to represent a critical period during which the protein intake of the dam can impact the offspring’s weight gain and feeding behavior. Animal models are also widely used to understand the processes and mechanisms that contribute to obesity at different physiological and pathophysiological stages. An interesting example of such aspect is the situation of decreased estrogen level occurring at menopause, which is linked to weight gain and decreased energy expenditure. To study metabolic disorders associated with such situation, estrogen withdrawal in ovariectomized animal models to mimic menopause are frequently used. According to many studies, clear species-specific differences exist between rats and mice that need to be taken into account when results are extrapolated to humans.

show abstract

“…Serum amino acids were determined by an Applied Biosystems 3200 Q TRAP liquid chromatography–tandem mass spectrometry (LC‐MS/MS) system equipped with an RP‐C18‐column (150 × 4.6 mm, 5 mm) as previously described …”

Section: Methodsmentioning

confidence: 99%

Negative effects on newborn piglets caused by excess dietary tryptophan in the morning in sows

Bai

Peng

et al. 2019

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUND This study investigated the effect of dynamic feeding models of dietary tryptophan on sows' performance during late pregnancy. RESULTS The average piglet birth weight and live farrowing rate from sows consuming a high–low tryptophan diet (0.39% Trp in the morning and 0.13% Trp in the afternoon) were decreased compared with those fed a 2×tryptophan diet (0.26% Trp in the morning and afternoon). Compared with the 2×tryptophan group, sow serum kynurenic acid and the newborn liver n‐6:n‐3 polyunsaturated fatty acid ratio were significantly higher, and sow serum taurine and newborn serum taurine, phosphoserine, cysteine and proline were lower in the high–low tryptophan diet group. Eighty‐eight genes were differentially expressed in newborn piglets' livers between the 2×tryptophan and high–low groups. Genes related to cytotoxic effector regulation (major histocompatibility complex class I proteins), NADH oxidation, reactive oxygen species (ROS) metabolism and tissue development were differentially expressed between these two groups. CONCLUSION Together, the results provide information on new biomarkers in serum or liver and provide novel insights into variations in the fetal liver during exogenous stimulus response and biological processes of ROS metabolism in fetuses during late pregnancy caused by a single excessive tryptophan ingestion daily in the morning. © 2018 Society of Chemical Industry

show abstract

Co-dependence of genotype and dietary protein intake to affect expression on amino acid/peptide transporters in porcine skeletal muscle

Cited by 27 publications

References 99 publications

Dietary mulberry leaf powder affects growth performance, carcass traits and meat quality in finishing pigs

Dietary mulberry leaf powder affects growth performance, carcass traits and meat quality in finishing pigs

Animal Models for the Study of the Relationships between Diet and Obesity: A Focus on Dietary Protein and Estrogen Deficiency

Negative effects on newborn piglets caused by excess dietary tryptophan in the morning in sows

Contact Info

Product

Resources

About