Co‐micellized Pluronic mixture with thermo‐sensitivity and residence stability as an injectable tissue adhesion barrier hydrogel

Abstract

Although the tissue adhesion which leads to various complications frequently occurs after surgery, the development of an ideal tissue adhesion barrier is still a challenge. In this study, a thermo-sensitive hydrogel, which can fulfill the essential requirements of tissue adhesion barrier (that is, ease of handling for surgeon, flowing down prevention after application, stable residence on the injury during wound healing, and no use of toxic additives), was developed using biocompatible polyethylene glycol-polypropylene glycol copolymers (Pluronic F127/F68/P123 mixture). From the in vitro cell culture and in vivo animal study, it was observed that the Pluronic mixtures showed sol-gel transition at approximately body temperature (for easy injection or coating on the injury site and flowing down prevention after application) and prolonged residence stability in aqueous environment (> ∼7 days for stable protection of injury tissues/organs during wound healing), and thus was highly effective for the prevention of tissue adhesion without adverse tissue responses. Based on these results, the Pluronic F127/F68/P123 mixture itself (without any additives) can be a good candidate as an injectable or coatable tissue adhesion barrier hydrogel applicable to various injury tissues in terms of ease of use, effectiveness, and safety. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 172-182, 2018.

Non-swellable F127-DA hydrogel with concave microwells for formation of uniform-sized vascular spheroids

“…They are widely used in cell culture and drug delivery for their nontoxic property, 20 and at the same time, most of Pluronics are non-adherent for cells due to the presence of non-fouling PEG chains. [21][22][23] In our previous study, we have prepared a nonswellable Pluronic F127-DA hydrogel which maintained the asprepared size and mechanical strength aer equilibrating at 37 C in culture medium. 24 In this study, the F127-DA will be fabricated into hydrogel with concave microwells for the formation of vascular spheroids, while the conventional PEG-DA hydrogel is set as control.…”

Advancement of Biomaterial‐Based Postoperative Adhesion Barriers

“…have developed Pluronic F127/F68/P123, including mildly crosslinked alginate and nonsteroidal anti‐inflammatory drug (ibuprofen), which showed gelation at physiological temperature, prolonged residence stability in an aqueous environment, and highly effective against PPA without adverse tissue responses, unlike other Pluronic hydrogels. [ 225 ]…”

Pre‐clinical and clinical applications of thermoreversible hydrogels in biomedical engineering: a review

“…197 Above its critical gelation concentration, gel formation is observed, due to the system reaching a sufficiently high volume fraction of micelles. 190 Other poloxamers that have been incorporated in in vivo injectable systems are (i) poloxamer 188 (P188), 58,[60][61][62][63][64][65][66][67][68][69][70][71][72][73]78,79,84,85,[88][89][90][91] (ii) poloxamer 124 (P124), 59 (iii) poloxamer 403 (P403), 58 (iv) poloxamer 338 (P338) 57 and (v) poloxamer 105 (P105) 106,107 (Table 2). The Pluronic® code is also included in Table 2; the letter describes the morphology of the polymer (F, P and L for flake, paste and liquid), the first (two) number(s) denote approximately the total MM in kg mol −1 , and the last number, when multiplied by 10, denotes the percentage of EG.…”