CO2-enriched microenvironment affects sucrose and macronutrients absorption and promotes autotrophy in the in vitro culture of kiwi (Actinidia deliciosa Chev. Liang and Ferguson)

Arigita, Luis; Cañal, Ma Jesús; Tamés, Ricardo Sánchez; González, A.

doi:10.1007/s11627-009-9267-x

Cited by 22 publications

(11 citation statements)

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“…73 Withdrawal of sucrose from the medium did not favour an increase in fresh and dry masses. This observation is consistent with the findings of Arigita et al with Kiwi explants 55 and Valero-Aracama et al in sea oats 56 under similar culture conditions. The reason behind this was that some cultures require an initial source of carbon from the medium until they are capable of using CO 2 from the vessel headspace as their main carbon source.…”

Section: Discussionsupporting

confidence: 93%

“…Similar synergistic effects of combinations of sucrose and CO 2 on in vitro shoot growth were observed in tobacco, 53 Wrightia tomentosa, 31 apple, 54 C. papaya 46 and Actinidia deliciosa. 55 Besides this, it was also observed that liquid medium was superior in overall growth over semi-solid medium in CO 2 -enriched conditions. CO 2 enrichment in liquid culture system was promotory for growth in Uniola paniculata 56 and Musa.…”

Section: Discussionmentioning

confidence: 87%

“…Fully photoautotrophic conditions promoted maximum plant growth in terms of both fresh and dry mass in Actinidia deliciosa. 55 In vitro culture under photoautotrophic conditions has been shown to promote the fresh and dry weight contents of several species, including, Phalaenopsis, Cymbidium kanran, and C. goeringii, 70 Hypericum perforatum, 33 Uniola paniculata 56 and Musa plantain. 35 Higher plantlet dry mass and contents of photosynthetic pigments, facilitated ex vitro acclimation and growth in Capsicum.…”

Section: Discussionmentioning

confidence: 99%

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Photosynthetic efficiency and in vitro growth of Celastrus paniculatus Willd. under varied concentrations of CO2

Habibi¹,

Purohit²

2019

Int J Phytocos Nat Ingred

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Introduction: Celastrus paniculatus (family: Celastraceae) is a woody climbing shrub valued for its immense medicinal properties contained in its various plant parts. Over-exploitation and poor natural regeneration either by seed or other method/s have resulted into depleting population of C. paniculatus in natural habitats in India. Novel approaches such as liquid culture media and photoautotrophic multiplication of shoots on sugar-free media has proved useful to obtain photosynthetically active micropropagated plantlets. Methods: The shoot cultures of C. paniculatus were multiplied on sucrose containing and sucrose-free semi-solid and liquid media. The cultures were further incubated under various concentrations of carbon dioxide (0.0 to 40.0 gm-3). The assessment of chlorophyll fluorescence parameters (F o , F m , F' m , F t , F v /F m and ΦPS2) under similar conditions was undertaken. Results: It was observed that CO 2 enrichment favored shoot multiplication and elongation and biomass production in sucrose supplemented medium. CO 2 (10.0 gm-3) along with sucrose (3.0%), recorded maximum growth on semi-solid and liquid media. The valuation of F o , F m , F' m , F t , F v /F m and ΦPS2 revealed that an increase in the concentration of CO 2 resulted in a decline in all the parameters especially the F v / F m and ΦPS2. On the contrary, withdrawal of sucrose from the medium under CO 2 enriched conditions resulted in a moderate growth rate and biomass production. However, F v /F m and ΦPS2 were considerably improved in shoot cultures incubated under elevated concentrations of CO 2 (10.0 gm-3) without sucrose in the medium indicating their photoautotrophic growth. Conclusion: Liquid medium proved to be superior for overall growth and biomass production over its semi-solid counterparts. The observations of photochemical efficiency in shoot cultures grown on liquid medium were at par with their semi-solid counterparts indicating no adverse effects such as hyperhydricity.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

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Photosynthetic efficiency and in vitro growth of Celastrus paniculatus Willd. under varied concentrations of CO2

Habibi¹,

Purohit²

2019

Int J Phytocos Nat Ingred

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“…CO 2 concentration inside the culture vessels decreased when plantlets grown in vitro, which limits the photosynthetic rate of the plants [4, 5]. The biomass accumulation of the in vitro cultured plants increased under photoautotrophic and CO 2 enrichment conditions, also affected nutrient absorption and secondary metabolism [6, 7].…”

Section: Introductionmentioning

confidence: 99%

Elevated CO2 concentration promotes photosynthesis of grape (Vitis vinifera L. cv. ‘Pinot noir’) plantlet in vitro by regulating RbcS and Rca revealed by proteomic and transcriptomic profiles

Zhao

Wang

et al. 2019

BMC Plant Biol

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BackgroundPlant photosynthesis can be improved by elevated CO2 concentration (eCO2). In vitro growth under CO2 enriched environment can lead to greater biomass accumulation than the conventional in micropropagation. However, little is know about how eCO2 promotes transformation of grape plantlets in vitro from heterotrophic to autotrophic. In addition, how photosynthesis-related genes and their proteins are expressed under eCO2 and the mechanisms of how eCO2 regulates RbcS, Rca and their proteins have not been reported.ResultsGrape (Vitis vinifera L. cv. ‘Pinot Noir’) plantlets in vitro were cultured with 2% sucrose designated as control (CK), with eCO2 (1000 μmol·mol− 1) as C0, with both 2% sucrose and eCO2 as Cs. Here, transcriptomic and proteomic profiles associated with photosynthesis and growth in leaves of V. vinifera at different CO2 concentration were analyzed. A total of 1814 genes (465 up-regulated and 1349 down-regulated) and 172 proteins (80 up-regulated and 97 down-regulated) were significantly differentially expressed in eCO2 compared to CK. Photosynthesis-antenna, photosynthesis and metabolism pathways were enriched based on GO and KEGG. Simultaneously, 9, 6 and 48 proteins were involved in the three pathways, respectively. The leaf area, plantlet height, qP, ΦPSII and ETR increased under eCO2, whereas Fv/Fm and NPQ decreased. Changes of these physiological indexes are related to the function of DEPs. After combined analysis of proteomic and transcriptomic, the results make clear that eCO2 have different effects on gene transcription and translation. RbcS was not correlated with its mRNA level, suggesting that the change in the amount of RbcS is regulated at their transcript levels by eCO2. However, Rca was negatively correlated with its mRNA level, it is suggested that the change in the amount of its corresponding protein is regulated at their translation levels by eCO2.ConclusionsTranscriptomic, proteomic and physiological analysis were used to evaluate eCO2 effects on photosynthesis. The eCO2 triggered the RbcS and Rca up-regulated, thus promoting photosynthesis and then advancing transformation of grape plantlets from heterotrophic to autotrophic. This research will helpful to understand the influence of eCO2 on plant growth and promote reveal the mechanism of plant transformation from heterotrophic to autotrophic.Electronic supplementary materialThe online version of this article (10.1186/s12870-019-1644-y) contains supplementary material, which is available to authorized users.

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“…In turmeric micropropagation, plant density and medium volume altered the amounts of nutrients used with different optimal mineral solutions required for high and low density cultures [ 20 ]. Sucrose concentration in media affects dry mass and the amount of NH 4 , NO 3 , Ca, Mg, and K taken up [ 22 ]. Multiplication and plant growth responses to plant density varied with medium volume and nutrient availability [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Growth of Curcuma longa L. in Response to Five Mineral Elements and Plant Density in Fed-Batch Culture Systems

2015

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Plant density was varied with P, Ca, Mg, and KNO3 in a multifactor experiment to improve Curcuma longa L. micropropagation, biomass and microrhizome development in fed-batch liquid culture. The experiment had two paired D-optimal designs, testing sucrose fed-batch and nutrient sucrose fed-batch techniques. When sucrose became depleted, volume was restored to 5% m/v sucrose in 200 ml of modified liquid MS medium by adding sucrose solutions. Similarly, nutrient sucrose fed-batch was restored to set points with double concentration of treatments’ macronutrient and MS micronutrient solutions, along with sucrose solutions. Changes in the amounts of water and sucrose supplementations were driven by the interaction of P and KNO3 concentrations. Increasing P from 1.25 to 6.25 mM increased both multiplication and biomass. The multiplication ratio was greatest in the nutrient sucrose fed-batch technique with the highest level of P, 6 buds/vessel, and the lowest level of Ca and KNO3. The highest density (18 buds/vessel) produced the highest fresh biomass at the highest concentrations of KNO3 and P with nutrient sucrose fed-batch, and moderate Ca and Mg concentrations. However, maximal rhizome dry biomass required highest P, sucrose fed-batch, and a moderate plant density. Different media formulations and fed-batch techniques were identified to maximize the propagation and storage organ responses. A single experimental design was used to optimize these dual purposes.

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CO2-enriched microenvironment affects sucrose and macronutrients absorption and promotes autotrophy in the in vitro culture of kiwi (Actinidia deliciosa Chev. Liang and Ferguson)

Cited by 22 publications

References 44 publications

Photosynthetic efficiency and in vitro growth of Celastrus paniculatus Willd. under varied concentrations of CO2

Photosynthetic efficiency and in vitro growth of Celastrus paniculatus Willd. under varied concentrations of CO2

Elevated CO2 concentration promotes photosynthesis of grape (Vitis vinifera L. cv. ‘Pinot noir’) plantlet in vitro by regulating RbcS and Rca revealed by proteomic and transcriptomic profiles

In Vitro Growth of Curcuma longa L. in Response to Five Mineral Elements and Plant Density in Fed-Batch Culture Systems

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