CO2 gradient domestication improved high-concentration CO2 tolerance and photoautotrophic growth of Euglena gracilis

Xin, Kai; Guo, Ruhan; Zou, Xiangbo; Rao, Mumin; Huang, Zhimin; Kuang, Cao; Ye, Ji; Chen, Chuangting; Huang, Cong; Zhang, Maoqiang; Yang, Weijuan; Cheng, Jun

doi:10.1016/j.scitotenv.2023.161629

Cited by 13 publications

(5 citation statements)

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“…Pigment extraction and determination were conducted as previously reported: chlorophyll a (Chl a ), b (Chl b ), and carotenoids’ contents were measured by ethanol extraction and spectrophotometry …”

Section: Methodsmentioning

confidence: 99%

“…Preparation of enzyme extracts was conducted as previously reported: Crude enzyme extracts were obtained using an ultrasonic cell disruptor (UH-S2, AUTO Science, China) for enzyme assays …”

Section: Methodsmentioning

confidence: 99%

“…Paramylon extraction of E. gracilis cells was conducted as previously reported . The paramylon content was tested using glucose as standard …”

Section: Methodsmentioning

confidence: 99%

“…Key Enzyme Expressions. Preparation of enzyme extracts was conducted as previously reported: 26 Crude enzyme extracts were obtained using an ultrasonic cell disruptor (UH-S2, AUTO Science, China) for enzyme assays. 28 Total protein concentrations were tested using the total protein assay kit (bicinchoninic acid method).…”

Section: Microalgal Strain and Cultivationmentioning

confidence: 99%

“…Pigment Content, Photosynthetic Fluorescence, and Photosynthetic Oxygenation. Pigment extraction and determination were conducted as previously reported: 26 chlorophyll a (Chl a), b (Chl b), and carotenoids' contents were measured by ethanol extraction and spectrophotometry. 27 The photosynthetic fluorescence parameters of E. gracilis cells (such as Fs, Fm′, Fo, Fm, and Fv/Fm) were directly measured by a pulse modulation fluorometer (FMS-2, Hansatech, UK).…”

Section: Microalgal Strain and Cultivationmentioning

confidence: 99%

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Photoautotrophic Growth and Cell Division of Euglena gracilis with Mixed Red and Blue Wavelengths

Xin,

Guo,

Wang

et al. 2024

Ind. Eng. Chem. Res.

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To improve flue gas CO 2 fixation of Euglena gracilis under a photoautotrophic environment, mixed red and blue wavelengths were used to promote photosynthetic growth and cell division with 15% CO 2 . The biomass dry weight increased by 385% to 1.31 g/L with a peak ribulose-1,5-bisphosphate carboxylase/oxygenase enzyme activity and oxygen release, when the ratio of red to blue wavelength decreased to 2:3. E. gracilis mainly engaged in photochemical reactions with accumulated photosynthetic pigment to focus on light conversion by optimizing the carotenoid/chlorophyll ratio and chlorophyll a/b ratio. The energy capture efficiency of open photosystem II (PSII) reaction centers and quantum efficiency of charge separation in PSII reaction centers were improved to maintain active photosynthesis. Blue light facilitated transition from G1 to S phase, while red light promoted transition from S to G2 phase. The malondialdehyde content decreased to a valley of 1.36 nmol/mg protein to keep efficient photosynthetic apparatus with carbon fixation.

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Section: Methodsmentioning

confidence: 99%

“…Preparation of enzyme extracts was conducted as previously reported: Crude enzyme extracts were obtained using an ultrasonic cell disruptor (UH-S2, AUTO Science, China) for enzyme assays …”

Section: Methodsmentioning

confidence: 99%

“…Paramylon extraction of E. gracilis cells was conducted as previously reported . The paramylon content was tested using glucose as standard …”

Section: Methodsmentioning

confidence: 99%

Section: Microalgal Strain and Cultivationmentioning

confidence: 99%

Section: Microalgal Strain and Cultivationmentioning

confidence: 99%

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