Cobalt content modulates characteristics and osteogenic properties of cobalt-containing hydroxyapatite in in-vitro milieu

Kahaie-Khosrowshahi, Amir; Khoshfetrat, Ali Baradar; Khosrowshahi, Younes Beygi; Maleki-Ghaleh, H.

doi:10.1016/j.mtcomm.2021.102392

Cited by 11 publications

(8 citation statements)

References 42 publications

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“…Considering the biomaterials used for bone implants and scaffolds, hydroxyapatite (HA) is one of the preferred choices [6,7]. Possessing chemical composition of Ca 10 (PO 4 ) 6 (OH) 2 , HA is one of the main components of bone (nearly 70%) which forms the mineral part of bones tissues [8]. Because HA is biocompatible, nontoxic, and non-inflammatory at the same time, it is one of the most favored biomaterials for fabricating bone scaffolds [9,10].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and Cellular Behaviors of Novel Zinc-Doped Hydroxyapatite/Graphene Nanocomposite for Bone Tissue Engineering

Maleki-Ghaleh

Siadati

Fallah

et al. 2021

IJMS

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Bacteria are one of the significant causes of infection in the body after scaffold implantation. Effective use of nanotechnology to overcome this problem is an exciting and practical solution. Nanoparticles can cause bacterial degradation by the electrostatic interaction with receptors and cell walls. Simultaneously, the incorporation of antibacterial materials such as zinc and graphene in nanoparticles can further enhance bacterial degradation. In the present study, zinc-doped hydroxyapatite/graphene was synthesized and characterized as a nanocomposite material possessing both antibacterial and bioactive properties for bone tissue engineering. After synthesizing the zinc-doped hydroxyapatite nanoparticles using a mechanochemical process, they were composited with reduced graphene oxide. The nanoparticles and nanocomposite samples were extensively investigated by transmission electron microscopy, X-ray diffraction, and Raman spectroscopy. Their antibacterial behaviors against Escherichia coli and Staphylococcus aureus were studied. The antibacterial properties of hydroxyapatite nanoparticles were found to be improved more than 2.7 and 3.4 times after zinc doping and further compositing with graphene, respectively. In vitro cell assessment was investigated by a cell viability test and alkaline phosphatase activity using mesenchymal stem cells, and the results showed that hydroxyapatite nanoparticles in the culture medium, in addition to non-toxicity, led to enhanced proliferation of bone marrow stem cells. Furthermore, zinc doping in combination with graphene significantly increased alkaline phosphatase activity and proliferation of mesenchymal stem cells. The antibacterial activity along with cell biocompatibility/bioactivity of zinc-doped hydroxyapatite/graphene nanocomposite are the highly desirable and suitable biological properties for bone tissue engineering successfully achieved in this work.

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Section: Introductionmentioning

confidence: 99%

Antibacterial and Cellular Behaviors of Novel Zinc-Doped Hydroxyapatite/Graphene Nanocomposite for Bone Tissue Engineering

Maleki-Ghaleh

Siadati

Fallah

et al. 2021

IJMS

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“…Numerous studies have shown that HA scaffolds could induce apatite deposition on the surface in SBF, which was related to the bioactivity properties of scaffolds[ 40 , 41 ]. The HA, CHA-0, and CHA-H scaffolds were soaked in the SBF for 14 days at 37°C, and then the surface morphologies were observed by SEM.…”

Section: Resultsmentioning

confidence: 99%

Zn-doped chitosan/alginate multilayer coatings on porous hydroxyapatite scaffold with osteogenic and antibacterial properties

He¹,

Chen²,

Wu³

et al. 2023

IJB

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Porous hydroxyapatite (HA) scaffolds prepared by three-dimensional (3D) printing have wide application prospects owing to personalized structural design and excellent biocompatibility. However, the lack of antimicrobial properties limits its widespread use. In this study, a porous ceramic scaffold was fabricated by digital light processing (DLP) method. The multilayer chitosan/alginate composite coatings prepared by layer-by-layer method were applied to scaffolds and Zn2+ was doped into coatings in the form of ion crosslinking. The chemical composition and morphology of coatings were characterized by scanning electron microscope (SEM) and X-ray photoelectron spectroscopy (XPS). Energy dispersive spectroscopy (EDS) analysis demonstrated that Zn2+ was uniformly distributed in the coating. Besides, the compressive strength of coated scaffolds (11.52 ± 0.3 MPa) was slightly improved compared with that of bare scaffolds (10.42 ± 0.56 MPa). The result of soaking experiment indicated that coated scaffolds exhibited delayed degradation. In vitro experiments demonstrated that within the limits of concentration, a higher Zn content in the coating has a stronger capacity to promote cell adhesion, proliferation and differentiation. Although excessive release of Zn2+ led to cytotoxicity, it presented a stronger antibacterial effect against Escherichia coli (99.4%) and Staphylococcus aureus (93%).

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“…In the results, SB-Ti and PEO-SB-Ti showed peaks of the HA with crystalline natures, while HA was not detected in Ti sample. [45][46][47] In addition, mainly titanium dioxide in the forms of anatase and rutile was detected in PEO-SB-Ti implant groups. 14 The oxide film formed on the surface provides excellent biocompatibility properties, and in particular, the anatase phase is known to play a role in promoting and helping the nucleation and growth of HA by matching each other in certain crystal plane with HA.…”

Section: Preparation Of Plasma Electrolytic Oxidation-treated Sandbla...mentioning

confidence: 99%

Hierarchically porous surface of HA-sandblasted Ti implant screw using the plasma electrolytic oxidation: Physical characterization and biological responses

Choe,

Li,

Yeo

et al. 2024

J Biomater Appl

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The surface topological features of bioimplants are among the key indicators for bone tissue replacement because they directly affect cell morphology, adhesion, proliferation, and differentiation. In this study, we investigated the physical, electrochemical, and biological responses of sandblasted titanium (SB-Ti) surfaces with pore geometries fabricated using a plasma electrolytic oxidation (PEO) process. The PEO treatment was conducted at an applied voltage of 280 V in a solution bath consisting of 0.15 mol L−1 calcium acetate monohydrate and 0.02 mol L−1 calcium glycerophosphate for 3 min. The surface chemistry, wettability, mechanical properties and corrosion behavior of PEO-treated sandblasted Ti implants using hydroxyapatite particles (PEO-SB-Ti) were improved with the distribution of calcium phosphorous porous oxide layers, and showed a homogeneous and hierarchically porous surface with clusters of nanopores in a bath containing calcium acetate monohydrate and calcium glycerophosphate. To demonstrate the efficacy of PEO-SB-Ti, we investigated whether the implant affects biological responses. The proposed PEO-SB-Ti were evaluated with the aim of obtaining a multifunctional bone replacement model that could efficiently induce osteogenic differentiation as well as antibacterial activities. These physical and biological responses suggest that the PEO-SB-Ti may have a great potential for use an artificial bone replacement compared to that of the controls.

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Cobalt content modulates characteristics and osteogenic properties of cobalt-containing hydroxyapatite in in-vitro milieu

Cited by 11 publications

References 42 publications

Antibacterial and Cellular Behaviors of Novel Zinc-Doped Hydroxyapatite/Graphene Nanocomposite for Bone Tissue Engineering

Antibacterial and Cellular Behaviors of Novel Zinc-Doped Hydroxyapatite/Graphene Nanocomposite for Bone Tissue Engineering

Zn-doped chitosan/alginate multilayer coatings on porous hydroxyapatite scaffold with osteogenic and antibacterial properties

Hierarchically porous surface of HA-sandblasted Ti implant screw using the plasma electrolytic oxidation: Physical characterization and biological responses

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