Cobalt has Enhancing Effect on Extracellular Lipases Isolated from Pseudomonas aeroginosa JCM5962(T)

Sachan, Shweta; Chandra, VanamYagnic; Yadu, Alka; Singh, Aditi

doi:10.20902/ijptr.2017.1016

Cited by 6 publications

(4 citation statements)

References 9 publications

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“…Researchers have noticed that the activity of P. aeruginosa JCM5962 (T) enzymes and other species of Pseudomonas decreases when the medium has a high Na + ion concentration (Gautam and Azmi 2017; Sachan et al . 2017).…”

Section: Resultsmentioning

confidence: 99%

“…As a result, under such conditions the bacterial cell membrane easily transports these ions into the cell and changes its osmotic pressure, which can affect the viability of the bacteria. Researchers have noticed that the activity of P. aeruginosa JCM5962 (T) enzymes and other species of Pseudomonas decreases when the medium has a high Na + ion concentration (Gautam and Azmi 2017;Sachan et al 2017). strategy of adding inoculum progressively was effective in improving the fermentation process.…”

Section: Resultsmentioning

confidence: 99%

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Lactobionic acid production by Pseudomonas taetrolens in a fed‐batch bioreactor using acid whey as substrate

Sarenkova

Sáez‐Orviz

Ciproviča

et al. 2022

Int J of Dairy Tech

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Acid whey causes a major disposal problem for the dairy industry due to its low pH and high salt concentration. Lactobionic acid (LBA) production by whey fermentation is an inexpensive process. The goal of this work was to employ acid whey for LBA production. Acid whey was fermented in a bioreactor, adding Pseudomonas taetrolens LMG 2336 at 30°C and 6.5 pH, with 1.5-Lpm oxygen aeration and 350-rpm agitation. Three fermentation tests were carried out with a different amount of inoculum (10%, 30% and 10% three times within 24 h). Results indicated that repeatedly adding P. taetrolens inoculum to the acid whey substrate allows a complete lactose conversion into LBA, while the lactose oxidation process was insufficient in the sample where the inoculum was only added at the beginning of the fermentation process (only 29.7% LBA production with 10% inoculum). The physiological heterogeneity of P. taetrolens was determined by multiparametric flow cytometry, and results showed that there was a phenotypic adaptation of the microorganism due to the changes observed in its heterogeneity and physiological state. The results achieved will help to recycle acid whey for value-added product production such as LBA production.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Lactobionic acid production by Pseudomonas taetrolens in a fed‐batch bioreactor using acid whey as substrate

Sarenkova

Sáez‐Orviz

Ciproviča

et al. 2022

Int J of Dairy Tech

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“…The internal osmotic pressure must be higher than in the external environment in order to maintain bacterial growth and viability. If osmotic pressure is too low/high, it could also affect enzyme activity [25,26]. To maintain an acid whey substrates pH, a significant amount of 1 M NaOH is used in samples with a higher proportion of acid whey (Figure 4), influencing lactonase activity and the lactose conversation rate into LBA.…”

Section: Figure 3 Correlation Between Lba and Salts Concentration In ...mentioning

confidence: 99%

Acid Whey Valorization for Biotechnological Lactobionic Acid Bio-production

Narala¹,

Zagorska

Sarenkova

et al. 2022

J. Hum. Earth Future

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The dairy industry is facing a problem associated with 1.6 billion tons of acid whey per year as a waste stream. The extended amount of acid whey has encouraged studies for novel approaches of acid whey utilization. The production of lactobionic acid (LBA) using dairy waste has been in rapid demand as an economically feasible and environmentally friendly approach. The composition of acid whey makes lactose conversion into LBA by Pseudomonas taetrolens complicated. Therefore, the aim of the current research was to evaluate factors (quality of whey (salts, protein concentration, pH), volume of inoculum, and cultivation time) with the purpose of increasing the suitability of acid whey for biotechnological LBA production. LBA production was performed in a 4L bioreactor, which was equipped with a pH electrode and a dissolved oxygen electrode. The whole experiment was performed at a temperature of 30 °C under 350 rpm agitation. The continuous aeration was set at 0.5 L/min. The current study presents the study of acid and sweet whey combinations in different ratios (100:0; 50:50; 60:40, 70:30, 80:20, respectively) inoculated with 10% or 30% v/v of fresh P. taetrolens inoculum reaching up to 59.9 ± 1% LBA yield during cultivation. Increasing protein and pH in a substrate slows down the lactose converting ability of P. taetrolens. Results demonstrated that increasing the acid whey amount in a substrate can affect the LBA yield, and a combination of sweet and acid whey could be a good solution for biotechnological LBA production using dairy waste. Doi: 10.28991/HEF-SP2022-01-04 Full Text: PDF

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“…It was not suprising since most metal ions and detergents are known to negatively impact on activity and stability of lipases [21]. Detergents and metal ions are known to have an influence on the biological function of enzymes, by enhancing or inhibiting their activity via various mechanisms, such as acting as an electron donor or acceptor and forming complexes with limited reactivity [22]. Like any other enzyme, inhibition of lipase activity by these metal ions is caused by inhibition of the catalytic site [19].…”

Section: Effect Of Ph and Temperature On Enzyme Stability And Actimentioning

confidence: 99%

Microbial Screening for Bio-delipidation System of Pre-treated Poultry Slaughterhouse Wastewater: Impact of Solvents, Metal Ions and Detergents on Lipase Activity

Mbulawa¹,

Ntwampe²,

Basitere³

et al. 2018

ASETH-18,ACABES-18 &Amp; EBHSSS-18 Nov. 19-20 2018 Cape Town (South Africa)

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This study reports on the microbial screening for a bio-delipidation system of lipid-rich slaughterhouse wastewater, and on the optimal conditions for lipase production and activity. In this study, swaps were collected from the poultry slaughterhouse discharge point for screening, isolation and characterisation of lipolytic microorganisms using molecular techniques. Bacillus cereus strains AB1 (BF3) and CC-1 (B3O) were identified using 16S rRNA techniques. Maximal lipase production for both strains was observed between pH 6-8 and 45-60 °C. Optimal lipase activity for BF3 and B30 was achieved at pH 8 and 60 °C, and at pH 8.83 and 45°C, respectively. After partial purification, increased activity was observed for BF3 and B30 strains. Solvents, metal ions and detergents (triclosan and trichlorocarbonilide) affected lipase activity. It was concluded that BF3 and B30 strains were suitable candidates for bio-delipidation systems.

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Cobalt has Enhancing Effect on Extracellular Lipases Isolated from Pseudomonas aeroginosa JCM5962(T)

Cited by 6 publications

References 9 publications

Lactobionic acid production by Pseudomonas taetrolens in a fed‐batch bioreactor using acid whey as substrate

Lactobionic acid production by Pseudomonas taetrolens in a fed‐batch bioreactor using acid whey as substrate

Acid Whey Valorization for Biotechnological Lactobionic Acid Bio-production

Microbial Screening for Bio-delipidation System of Pre-treated Poultry Slaughterhouse Wastewater: Impact of Solvents, Metal Ions and Detergents on Lipase Activity

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