Cockroach sensitization in laboratory workers

Dr, Steinberg; Di, Bernstein; Js, Gallagher; Arlian, Larry G.; Il, Bernstein

doi:10.1016/0091-6749(87)90012-1

Cited by 26 publications

(11 citation statements)

References 11 publications

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“…In a study of 35 patients in the Boston area, there was concordance of skin test reactivity to commercial extracts of either German or American cockroach [2], In another study, cockroach-specific IgE was mea sured in the sera of 13 cockroach-sensitive patients, and it was suggested that the cockroach cross-reactiv ity may have been due to exposure to multiple species and that most of the allergenic activity of the German cockroach could be extracted from whole bodies and cast skins [1], Other studies have documented the high prevalence of cutaneous reactivity to cockroach al lergens among urban patients with asthma and the presence of both immediate-and dual-phase bronchoconstrictive responses in patients with extrinsic asthma following bronchial challenge with cockroach allergens [3,4,8], Recently, a clinical and immuno logic study on cockroach-laboratory workers demon strated that American cockroach hemolymph pos sessed a broad range of cross-reactivity not only with American cockroach fecal antigens but with a whole range of whole body allergens from different cock roach species, as well as with a beetle larval antigen [14] . These data supported the findings of Baur et al [15] , who suggested that increases in asthmatic disease reported in the Sudan and other areas were predomi nantly caused by sensitization to hemoglobin mole cules of chironomids.…”

Section: Discussionmentioning

confidence: 99%

Identification of a German Cockroach-Specific Allergen by Human IgE and Rabbit IgG

Helm¹,

Bandele²,

Swanson³

et al. 1988

Int Arch Allergy Immunol

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To further study the immunology of insect hypersensitivity, we identified and partially characterized the principal allergens in whole body German cockroach (WBGCR) (Blattella germanica) and compared this extract to whole body antigens prepared from other insects. WBGCR extract was fractionated over a calibrated Sephadex G-200 column; peak allergenic activity was contained in fraction 3 (GCR3), containing components with apparent molecular weights ranging from 12,500 to 75,000 daltons. The antigenicity, allergenicity, and specificity of GCR3 components were tested by using rabbit antisera raised to GCR3 or true armyworm (Pseudaletia unipuncta). Radioimmunoassay, cross-inhibition and immunoblot studies revealed, particularly in the IgE system, that GCR3 contained an allergen with a pI of 6.7 and MW of 36,000 daltons that was unique to WBGCR extracts and not present in other insect species, including true armyworm, caddis fly, lakefly, yellow jacket, or honeybee. This GCR3 component may represent a specific marker for the diagnosis of cockroach hypersensitivity in an insect-sensitive population of individuals.

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Section: Discussionmentioning

confidence: 99%

Identification of a German Cockroach-Specific Allergen by Human IgE and Rabbit IgG

Helm¹,

Bandele²,

Swanson³

et al. 1988

Int Arch Allergy Immunol

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“…among entomologists and laboratory personnel who work with CR (1-4). In urban or "inner city" areas, up to 60% of patients with asthma have IgE antibodies to CR allergens, as demonstrated by skin tests, bronchial challenge tests, and serum IgE antibody assays (1)(2)(3)(4). Delayed bronchial reactions involving eosinophil recruitment, a characteristic immunopathologic feature of asthma, also occur following inhalation of CR extract (5).…”

mentioning

confidence: 99%

Cloning of Cockroach Allergen, Bla g 4, Identifies Ligand Binding Proteins (or Calycins) as a Cause of IgE Antibody Responses

Arruda

Vailes

Hayden

et al. 1995

Journal of Biological Chemistry

130

118

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An allergen cloned from a Blattella germanica (German cockroach) cDNA library, encoded a 182-amino acid protein of 20,904 Da. This protein, designated B. germanica allergen 4 (Bla g 4), was expressed as a glutathione S-transferase fusion protein in Escherichia coli and purified by affinity chromatography and high-performance liquid chromatography. The prevalence of serum IgE antibody to recombinant Bla g 4 in 73 cockroach allergic patients with asthma ranged from 40% (antigen binding radioimmunoassay) to 60% (plaque immunoassay). Cockroach allergic patients gave positive intradermal skin tests to recombinant Bla g 4 at concentrations of 10 ؊3 -10 ؊5 g/ml, whereas non-allergic controls, or cockroach allergic patients with no detectable serum IgE antibody to Bla g 4, gave negative skin tests to 1 g/ml. Polymerase chain reaction and Southern analysis identified a 523-base pair DNA encoding Bla g 4 in both B. germanica and Periplaneta americana (American cockroach). However, Northern analysis showed that mRNA encoding Bla g 4 was transcribed in B. germanica but not in P. americana, suggesting that allergen expression was species specific. Sequence similarity searches showed that Bla g 4 was a ligand binding protein or calycin and unexpectedly revealed that this family contained several important allergens: ␤-lactoglobulin, from cow milk, and rat and mouse urinary proteins. Although the overall sequence homology between these proteins was low (ϳ20%), macromolecular modeling techniques were used to generate two models of the tertiary structure of Bla g 4, based on comparisons with the x-ray crystal coordinates of bilin binding protein and rodent urinary proteins. The results show that members of the calycin protein family can cause IgE antibody responses by inhalation or ingestion and are associated with asthma and food hypersensitivity.

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“…Conversely, the Asian extract showed little inhibitory capacity with either D/H-S Am or D/H-S Or cock roach extract solid-phase polymers and IgE serum pool. Although direct RAST indicated that extracts of whole bodies, cast skins, and egg cases of the German cockroach contained the clinically relevant allergens [4], RAST inhibition studies demonstrated cross-inhi bition to American hemolymph by American, Ger man, and Brown-banded cockroach whole-body ex tracts [22], Collectively, these results suggest that the allergen concentration and/or presentation exposed on the polymers may vary significantly and lead to an oversimplification or misinterpretation of the results. Shared epitopes on allergens may be present among all cockroach species and could be dependent on dif ferences in the respective allergen concentration.…”

Section: Discussionmentioning

confidence: 87%

Shared Allergenic Activity in Asian (Blattella asahinai), German (Blattella germanica), American (Periplaneta americana), and Oriental (Blatta orientalis) Cockroach Species

Helm

Squillace

Jones

et al. 1990

Int Arch Allergy Immunol

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Whole-body extracts of the feral and peridomestic Asian cockroach (Blattella asahinai) and the three domestic cockroach species, German (Blattella germanica), American (Periplaneta americana), and Oriental (Blatta orientalis), were compared allergenically using an IgE serum pool from 4 German cockroach sensitive individuals. In crossover radioallergosorbent inhibition analysis, the Asian cockroach shared allergenic activity primarily with the German cockroach polymer and to a lesser extent with either the American or Oriental cockroach polymers. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and thin-layer isoelectric focusing analysis of the extracts showed similar although varying intensities of Coomassie blue stained banding patterns among five extracts analyzed. Electroblotting analysis with 12.5% SDS-PAGE of the whole-body German cockroach extract and IgE serum from individuals sensitive to German cockroach revealed eight allergenic components with apparent molecular weights of 92, 80, 67, 48, 36, 27, 25 and 18 kD. Five components could be identified in the whole-body extract of the Asian cockroach corresponding to apparent molecular weights of 92, 67, 48, 40, and 32 kD. Analysis of individual serum by immunoblot analysis with each of the cockroach extracts showed considerable heterogenicity in the IgE-binding pattern. Although the Asian cockroach demonstrated considerable cross-reacting allergenic components to German and relatively fewer cross-reacting allergenic components to either the Oriental or American, it is too early to establish genus- or species-specific cockroach allergens. It is important to point out that German cockroach sensitive individuals should be made aware of the potential exposure of Asian cockroach aeroallergens both indoors and outdoors in areas with high infestations of Asian cockroaches.

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Cockroach sensitization in laboratory workers

Cited by 26 publications

References 11 publications

Identification of a German Cockroach-Specific Allergen by Human IgE and Rabbit IgG

Identification of a German Cockroach-Specific Allergen by Human IgE and Rabbit IgG

Cloning of Cockroach Allergen, Bla g 4, Identifies Ligand Binding Proteins (or Calycins) as a Cause of IgE Antibody Responses

Shared Allergenic Activity in Asian <i>(Blattella asahinai)</i>, German <i>(Blattella germanica)</i>, American <i>(Periplaneta americana)</i>, and Oriental <i>(Blatta orientalis)</i> Cockroach Species

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