Coelocentesis: a new technique for early prenatal diagnosis

Jurkovic, D.; Jauniaux, Eric; Campbell, Stuart; Pandya, Pranav; Nicolaides, Kypros H.; Cardy, Don

doi:10.1016/0140-6736(93)90761-5

Cited by 62 publications

(49 citation statements)

References 7 publications

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“…cannot be successfully cultured, embryonic genetic material could be analyzed using PCR techniques. 97 …”

Section: Denaturant Gradient Gel Electrophoresismentioning

confidence: 99%

“…97 These cells have been initially used to determine fetal sex, sickle cell disease (SCD) 101 and other single-gene defects but often a diagnosis was impossible for high rate of maternal contamination. The main problems encountered during prenatal diagnosis using CF are the following: -The number of fetal cells.…”

Section: Coelomic Fluid Characteristics and Limitations For Prenatal mentioning

confidence: 99%

See 1 more Smart Citation

Prenatal Diagnosis of Hemoglobinopathies: From Fetoscopy to Coelocentesis

Damiani

Vinciguerra

Jakil

et al. 2014

Thalassemia Reports

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Prenatal diagnosis of hemoglobinopathies involves the study of fetal material from blood, amniocytes, trophoblast coelomatic cells and fetal DNA in maternal circulation. Its first application dates back to the 70s and it involves globin chain synthesis analysis on fetal blood. In the 1980s molecular analysis was introduced as well as amniocentesis and chorionic villi sampling under high-resolution ultrasound imaging. The application of direct sequencing and polymerase chain reactionbased methodologies improved the DNA analysis procedures and reduced the sampling age for invasive prenatal diagnosis from 18 to 16-11 weeks allowing fetal genotyping within the first trimester of pregnancy. In the last years, fetal material obtained at 7-8 weeks of gestation by coelocentesis and isolation of fetal cells has provided new platforms on which to develop diagnostic capabilities while non-invasive technologies using fetal DNA in maternal circulation are starting to develop.

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“…cannot be successfully cultured, embryonic genetic material could be analyzed using PCR techniques. 97 …”

Section: Denaturant Gradient Gel Electrophoresismentioning

confidence: 99%

Section: Coelomic Fluid Characteristics and Limitations For Prenatal mentioning

confidence: 99%

Prenatal Diagnosis of Hemoglobinopathies: From Fetoscopy to Coelocentesis

Damiani

Vinciguerra

Jakil

et al. 2014

Thalassemia Reports

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“…This knowledge may be used to differentiate fluids originating from these cavities and to understand the role of biochemical transfers between them. This knowledge may also be relevant in view of potential new applications, like coelocentesis for early prenatal diagnosis or fetal somatic gene therapy by injection into the exocoelomic cavity [4,5]. All these techniques are still in the experimental phase, and welldocumented animal models may contribute to further develop these.…”

Section: Introductionmentioning

confidence: 99%

Biochemical Differentiation of Gestational Compartments in the Midgestational Fetal Rabbit

Devlieger¹,

Gratacós²,

Wu³

et al. 2001

Fetal Diagn Ther

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Objectives: The fetal rabbit at midgestation is increasingly being used as a model in fetal diagnosis and therapy. In this study, we aimed to establish a reliable method for identification of the origin of sampled extra-embryonic fluids based on selected biochemical components. Methods: In 6 pregnant does at 22 days of gestation, 18 gestational sacs were sampled for amniotic, allantoic and exocoelomic fluid. These fluids, as well as matching maternal and fetal blood samples, were assayed for levels of sodium, potassium, chloride, bicarbonate, total protein, alkaline phosphatase, γ-glutamyl transferase and progesterone. Results: Levels of sodium and potassium were, respectively, lower and higher in the allantoic fluid when compared to other extra-embryonic spaces. Amniotic fluid had a significantly lower total protein content and higher level of alkaline phosphatase when compared to the exocoelomic fluid. Significant levels of progesterone could only be detected in maternal blood. Conclusions: In the midgestational rabbit, a combined assay of potassium, alkaline phosphatase and progesterone can determine the gestational cavity of origin of the sampled fluid. The obtained gradients for these markers suggest compartment-specific production and/or inter-cavity transfer mechanisms.

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confidence: 99%

“…Sonography has enabled in-vivo observation of the normal changes with time of the gestational sac compartments 1 -4 and sampling of amniotic and extra-embryonic celomic fluids in humans and non-human primates 5,6 . Investigators from King's College in London were the first to report on the feasibility of ultrasound-guided celocentesis in humans prior to elective termination of pregnancy 5 . Since then, celocentesis has: provided insights into the biochemical composition of celomic fluid 7,8 ; enabled the prenatal diagnosis of genetic disorders in very early pregnancy 9 ; and opened the doors to experimental in-utero stemcell therapy 10 .…”

mentioning

confidence: 99%