Coherent fluctuation nephelometry as a promising method for diagnosis of bacteriuria

Гурьев, А. С.; Yudina, Irina E.; Lazareva, Anna; Volkov, A. Yu.

doi:10.1016/j.plabm.2018.e00106

Cited by 3 publications

(1 citation statement)

References 14 publications

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“…Валидация метода исследования на отечественном КФН-анализаторе проводилась в сравнении с зарегистрированным на отечественном рынке анализатором UF-1000i. Исследования проводились на 117 образах мочи детей с инфекцией мочевыделительной системы [8], 18% были положительны по результатам посева на плотные среды. Результаты тестирования на КФНанализаторе одновременно обоими способами (по кривым роста и концентрации микроорганизмов) и на UF-1000i только по концентрации микробов сравнивали с результатами посева на плотные питательные среды, которые принимали за эталон (100%).…”

Section: опыт использования когерентной флуктуационной нефелометрии дunclassified

Coherent fluctuation nephelometry in clinical microbiology

Гурьев

Шалатова

Русанова

et al. 2019

Russian Journal of Infection and Immunity

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In this article data concerning coherent fluctuation nephelometry (CFN) use in clinical microbiology is presented. CFN-analyzer allows to solve two important problems – fast urine screening for bacteriuria within 2-4 hours and antibiotic susceptibility testing within 3-6 hours. Altogether more than 650 urine samples were tested, and the effectivity of CFN-analyzer for preliminary selection of samples for further analysis was shown. Method allows to detect negative samples, reducing the number of urine analyses by 70-80%. Simultaneous analysis of growth curves and concentration of microorganisms shows high sensitivity and specificity (95.2% и 96.9%). Also more than 250 antibiotic susceptibility tests were performed using CFN-analyzer to show its effectiveness for determination of resistant properties of both pure cultures and urine microflora without isolation of bacteria. The agreement with traditional methods was from 84% to 88%. The use of CFN-analyzer with express methods of identification of microorganisms (chromogenic nutrient broths or mass-spectrometry) allows to make full urine analysis within 1-2 days. In the future CFN-analyzer gives an opportunity to screen different human biological liquids, and finds an application for other microbiological tasks, including standardization and speeding-up in sanitary bacteriology.

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Section: опыт использования когерентной флуктуационной нефелометрии дunclassified

Coherent fluctuation nephelometry in clinical microbiology

Гурьев

Шалатова

Русанова

et al. 2019

Russian Journal of Infection and Immunity

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Fast antibiotic susceptibility testing of urine microflora using a microbiological analyzer based on coherent fluctuation nephelometry

et al. 2022

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Preoperative bacteriuria positivity on urinalysis increases wound complications in primary total hip arthroplasty regardless of the urine culture result

Peng

Zeng

et al. 2021

BMC Musculoskelet Disord

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Background Current evidence does not recommend screening urine culture and curing asymptomatic bacteriuria (ASB) before joint arthroplasty. The bacteriuria count on pre-operative urinalysis is a more common clinical parameter. We aimed to investigate whether the bacteriuria count on preoperative urinalysis can increase postoperative wound complications in primary total hip arthroplasty (THA). Methods We conducted a retrospective study that included patients who underwent primary THA in our institution from 2012 to 2018. We obtained preoperative urinalysis results before THA during the same hospitalization and identified patients with abnormal urinalysis. Receiver operating characteristic (ROC) curves were first generated to evaluate the predicted value of leukocyte esterase (LE), nitrite, bacteriuria, and pyuria in the urinalysis for superficial wound infection. Then, all included patients were divided into two groups according to the preoperative urinalysis: a bacteriuria-positive group and a bacteriuria-negative group. The primary outcome was the superficial wound infection rate within 3 months postoperatively, and the secondary outcomes included wound leakage, prosthetic joint infection (PJI), pulmonary infection, urinary tract infection (UTI), readmission rate within 3 months postoperatively, and length of stay (LOS) during hospitalization. We utilized univariable analyses to compare the outcomes between the two groups. A multivariable logistic regression model was generated to explore the potential association between bacteriuria and the risk of superficial wound infection, wound leakage, and readmission rate controlling for baseline values. Results A total of 963 patients were included in the study. One hundred sixty patients had abnormal urinalysis. The AUCs for LE, nitrite, bacteriuria, and pyuria were 0.507 (95% confidence interval (CI), 0.315 to 0.698), 0.551 (0.347 to 0.756), 0.675 (0.467 to 0.882), and 0.529 (0.331 to 0.728), respectively. Bacteriuria was diagnostically superior to LE, nitrite, and pyuria. Among the 963 patients, 95 had a positive bacteriuria on preoperative urinalysis, and only 9 (9.5%) had a positive urine culture. Compared with the bacteriuria-negative group, the bacteriuria-positive group had a higher superficial wound infection rate (4.2% vs. 0.6%, P = 0.008), higher wound leakage rate (11.6% vs. 4.5%, P = 0.007), higher readmission rate (5.3% vs. 1.3%, P = 0.015) within 3 months postoperatively and longer LOS (6.19 ± 2.89 days vs. 5.58 ± 2.14 days, P = 0.011). After adjustment, the bacteriuria-positive group had a significantly increased risk of superficial wound infection (OR = 7.587, 95%CI: 2.002 to 28.755, P = 0.003), wound leakage (OR = 3.044, 95%CI: 1.461 to 6.342, P = 0.003), and readmission (OR = 4.410, 95%CI: 1.485 to 13.097, P = 0.008). Conclusion Preoperative bacteriuria positivity on urinalysis significantly increased the risk of postoperative wound complications, readmission, and LOS in primary THA regardless of the result of the urine culture. Urinalysis is a fast and cost-acceptable test whose advantages have been underestimated. Level of evidence Level III, observational study.

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Coherent fluctuation nephelometry as a promising method for diagnosis of bacteriuria

Cited by 3 publications

References 14 publications

Coherent fluctuation nephelometry in clinical microbiology

Coherent fluctuation nephelometry in clinical microbiology

Fast antibiotic susceptibility testing of urine microflora using a microbiological analyzer based on coherent fluctuation nephelometry

Preoperative bacteriuria positivity on urinalysis increases wound complications in primary total hip arthroplasty regardless of the urine culture result

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