Coherent super-resolution microscopy via laterally structured illumination

Illumination plays an important role in optical microscopy. Köhler illumination, introduced more than a century ago, has been the backbone of optical microscopes. The last few decades have seen the evolution of new illumination techniques meant to improve certain imaging capabilities of the microscope. Most of them are, however, not amenable for wide-field observation and hence have restricted use in microscopy applications such as cell biology and microscale profile measurements. The method of structured illumination microscopy has been developed as a wide-field technique for achieving higher performance. Additionally, it is also compatible with existing microscopes. This method consists of modifying the illumination by superposing a well-defined pattern on either the sample itself or its image. Computational techniques are applied on the resultant images to remove the effect of the structure and to obtain the desired performance enhancement. This method has evolved over the last two decades and has emerged as a key illumination technique for optical sectioning, super-resolution imaging, surface profiling, and quantitative phase imaging of microscale objects in cell biology and engineering. In this review, we describe various structured illumination methods in optical microscopy and explain the principles and technologies involved therein.

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“…This has been reported to achieve better spatial resolution [87][88][89][90][91][92][93] or greater depth of field [94,95].…”

Section: Super-resolutionmentioning

confidence: 99%

Structured illumination microscopy

2015

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“…Others have used coherent structured illumination to reconstruct an enlarged field spectrum of the object but these approaches require complicated numerical postprocessing and they did not show the separation of objects below the 1 μm scale [4,5]. Recently, very high lateral resolution has been shown in coherent imaging using a holographic detection scheme [6].…”

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confidence: 99%

Coherent total internal reflection dark-field microscopy: label-free imaging beyond the diffraction limit

Olshausen

Rohrbach

2013

Opt. Lett.

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Coherent imaging is barely applicable in life-science microscopy due to multiple interference artifacts. Here, we show how these interferences can be used to improve image resolution and contrast. We present a dark-field microscopy technique with evanescent illumination via total internal reflection that delivers high-contrast images of coherently scattering samples. By incoherent averaging of multiple coherent images illuminated from different directions we can resolve image structures that remain unresolved by conventional (incoherent) fluorescence microscopy. We provide images of 190 nm beads revealing resolution beyond the diffraction limit and slightly increased object distances. An analytical model is introduced that accounts for the observed effects and which is confirmed by numerical simulations. Our approach may be a route to fast, label-free, super-resolution imaging in live-cell microscopy.

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“…Even though structured illumination can not achieve super-resolution in coherent imaging modalities that utilize simple scattering, it has been utilized for synthesizing a higher numerical aperture system using low numerical aperture objective. This has been reported to achieve better spatial resolution [87][88][89][90][91][92][93] or greater depth-of-field [94,95].…”

Section: Super-resolutionmentioning

confidence: 99%

Structured Illumination Microscopy

2016

Super-Resolution Imaging in Biomedicine

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Coherent super-resolution microscopy via laterally structured illumination

Cited by 34 publications

References 7 publications

Structured illumination microscopy

Structured illumination microscopy

Coherent total internal reflection dark-field microscopy: label-free imaging beyond the diffraction limit

Structured Illumination Microscopy

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