COL5A2 as a potential clinical biomarker for gastric cancer and renal metastasis

Abstract

Background: Gastric cancer, characterized by insidious onset and multiple metastasis, is almost incurable and has poor prognosis, and also one of the leading causes of treatment failure and death in patients with gastric cancer (GC). However, the prognosis of collagen type V alpha2 chain (COL5A2) in GC and renal metastasis is unknown. Methods: Recruited 148 patients who underwent GC. The diagnosis of GC was confirmed by ultrasound imaging and pathological examination. Immunohistochemistry and RT-qPCR were performed to exam the expression level of COL5A2. The statistical methods included Pearson chi-square test, Spearman-rho correlation test, univariate and multivariate cox regression analysis. Finally, this research constructed receiver operating characteristic (ROC) curves and applied the area under the curve (AUC). Results: Based on Pearson's chi-square test, Spearman-rho test, and univariate/multivariate cox regression, pathologic grade (P < .001), renal metastasis (P < .001) and staging (P < .001) were significantly related to COL5A2. And COL5A2 expression (hazard ratio [HR]: 18.834, P < .001) is an independent risk factor of GC. The AUC was used as the degree of confidence in judging each factor: COL5A2 (AUC = 0.878, P < .001), COL1A1 (AUC = 0.636, P = .006), COL1A2 (AUC = 0.545, P = .368), and COL3A1 (AUC = 0.617, P = .019). Through the ROC result, COL5A2 had more advantage as a biomarker for GC than other collagens. Conclusions: COL5A2 gene expression level might be a risk factor for GC. COL5A2 has a strong correlation with the prognosis of the disease.

Collagen Family and Other Matrix Remodeling Proteins Identified by Bioinformatics Analysis as Hub Genes Involved in Gastric Cancer Progression and Prognosis

“…Recent studies associate these molecules with processes such as migration, invasion, and poor overall survival. Moreover, inhibition of the gene expression reduces cell proliferation and invasion [ 34 , 37 , 38 , 39 , 40 , 41 , 42 ]. The overexpression of biglycan (BGN) was also identified in GC and was associated with poor prognosis, while inhibition of BGN enhanced chemotherapeutic efficacy.…”

Integrative analysis and experiments to explore angiogenesis regulators correlated with poor prognosis, immune infiltration and cancer progression in lung adenocarcinoma

“…Collagen type V alpha 2 chain (COL5A2) has previously been reported to be involved in various pathological processes in tumorigenesis and cancer progression, such as cancer cell survival, migration, immune microenvironment, angiogenesis and blood vessel development [ 25 , 26 ]. The expression level of COL5A2 can also effectively predict patient outcomes in multiple malignant tumors, including gastric cancer, bladder cancer and tongue squamous cell carcinoma [ 27 – 29 ]. EPHB2 is a member of the erythropoietin-producing hepatocellular (Eph) receptor family, which plays a critical role in various physiological and pathological functions, such as cell adhesion and migration, stemness and angiogenesis [ 30 – 32 ].…”

Cross-Talk of Focal Adhesion-Related Gene Defines Prognosis and the Immune Microenvironment in Gastric Cancer

“…EGF mediates the activation of Rab35 by DENND1A to regulate GC invasion and migration (Ye et al, 2018). A retrospective study found that COL5A2 was a potential risk factor for prognosis in GC patients (P < 0.001, HR = 18.834) (Ding et al, 2021). The above genes have been confirmed to be important participants in the development of GC, and we are also the first to find that they are important markers for GC stratification from a holistic perspective.…”