Colchicine blocks β adrenoceptor and class I antigen-specific interactions

Cremaschi, Graciela; Genaro, Ana Marı́a; Sterin‐Borda, Leonor

doi:10.1016/0161-5890(89)90042-4

Cited by 12 publications

(3 citation statements)

References 28 publications

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“…The cells were cultured at optimal concentrations of 3 × 10 5 cells/mL in RPMI 1640 medium (Gibco) supplemented with 10% fetal calf serum (FCS), 2 m m glutamine and antibiotics (Anesini et al ., 1996). Normal lymphoid cell suspensions were prepared from (BALB/c (H‐2d) inbred male mice (2–4 months old); to do this, lymph nodes were obtained aseptically from nylon wool purification of T cells as described previously (Cremaschi et al ., 1989). Normal cells, at a concentration of 1 × 10 6 cells/mL, were cultured in the same medium as tumor cells.…”

Section: Methodsmentioning

confidence: 99%

Comparative antioxidant activity of an extract of Lithraea molleoides and an isolated 5‐alkyl resorcinol derivative. Effects on the proliferation of normal and tumoral lymphocytes

López

Ferraro

Anesini

2011

Phytotherapy Research

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Lithraea molleoides (Vell.) Engl. (Anacardiaceae), is known in South America for its medicinal properties: antiarthritic, haemostatic, diuretic, tonic and useful for the treatment of respiratory diseases. Previously the isolation of a new cytotoxic 5-alkyl resorcinol derivative, 1,3-dihydroxy-5-(tridec-4',7'-dienyl) benzene, from a dichloromethane extract (DM) was reported. The aim of this work was to determine and to compare the antioxidant activity of DM and the isolated compound, 1,3-dihydroxy-5-(tridec-4',7'-dienyl) benzene. Moreover, the activity of both on the proliferation of tumoral and normal lymphocytes was determined. The compound was isolated and quantified by HPLC. The compound represented 0.036% of DM. The extract and the compound exerted antioxidant activity by DPPH and FTC methods. On tumoral cells, both exerted antiproliferative action but the compound was more active (EC(50) : 17.4 ± 1 µg/mL). On normal lymphocytes, both exerted a stimulatory effect on cell proliferation inversely related to concentration, the extract was more active than the compound (maximum effect: 300 ± 20% of stimulation). Most of the effects observed with the extract were independent of the isolated compound. DM could be an important source of antioxidant and immunomodulatory compounds to be studied on cancer diseases.

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Section: Methodsmentioning

confidence: 99%

Comparative antioxidant activity of an extract of Lithraea molleoides and an isolated 5‐alkyl resorcinol derivative. Effects on the proliferation of normal and tumoral lymphocytes

López

Ferraro

Anesini

2011

Phytotherapy Research

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“…The cells were cultured at optimal concentrations of 3 × 10 5 in RPMI 1640 medium (GIBCO supplemented with 10% fetal calf serum (FCS), 2 nM glutamine and antibiotics, (Anesini et al, 1996). Lymphoid cell suspensions from C3H (H-2d) inbred male mice (between 2-4 months old) lymph nodes were obtained aseptically from nylon wool purification of T-cells as described previously (Cremaschi et al, 1989). T-cell purity was higher than 97% as checked by lysis with anti-Thy plus complement and by indirect immunofluorescence.…”

Section: Cell Suspensions and Culture Conditionsmentioning

confidence: 99%

Comparative immunomodulatory effect of scopoletin on tumoral and normal lymphocytes

et al. 2006

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“…Lymphoid cell suspensions from BALB/c inbred mice lymph nodes, as well as nylon wool purified T cells, were prepared aseptically as described before [13,14]. Cells were cultured at a concentration of I x 106 cell/ml, in RPMI 1640 medium (Gibco Co.) supplemented with 10% heat-inactivated fetal calf serum, 2 mM glutamine and antibiotics, alone or in the presence of one of the stimulators of proliferation: Con A (4/lg/ml; Sigma Chemical Co.) or the phorbol ester, phorbol myristate acetate (PMA, 2 x 10 ~ M; Sigma) alone, together with ionophore A23187 (2 x 10 6 M; Sigma) or with 12.5 IU/ml of human recombinant IL-2 (rIL-2) (Electro Nucleonics Inc.).…”

Section: Cell Suspensions and Culture Conditionsmentioning

confidence: 99%

Down‐regulation of β‐adrenergic receptors induced by mitogen activation of intracellular signaling events in lymphocytes

1995

FEBS Letters

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The expression of/3-adrenergic receptors on murine lymphoeytes stimulated with concanavalin A was studied. A decrease in 13-adrenoeeptor number on T lymphoeytes and a diminished response to specific agonist stimulation at the peak of proliferatiou was found. The blockade of cell proliferation by tyrosine kinascs or protein kinase C inhibitors reversed the decrease in 13-adrenoceptor number. PMA plus ionophore or interleukin-2 hut not PMA alone were able to induce l]-adrenoceptor downregulation accompanying cellular proliferation. These results showed that the intraccllular signals triggered during lymphocyte activation are involved in/3-adrenoceptor down-regulation and it would represent the loss of a mechanism that exerts negative neuroimmune control of cellular proliferation.

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Colchicine blocks β adrenoceptor and class I antigen-specific interactions

Cited by 12 publications

References 28 publications

Comparative antioxidant activity of an extract of Lithraea molleoides and an isolated 5‐alkyl resorcinol derivative. Effects on the proliferation of normal and tumoral lymphocytes

Comparative antioxidant activity of an extract of Lithraea molleoides and an isolated 5‐alkyl resorcinol derivative. Effects on the proliferation of normal and tumoral lymphocytes

Comparative immunomodulatory effect of scopoletin on tumoral and normal lymphocytes

Down‐regulation of β‐adrenergic receptors induced by mitogen activation of intracellular signaling events in lymphocytes

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