Cold air alters MUC5AC and MUC5B expression in the airways of asthma patients

Naumov, Denis; Gassan, Dina; Kotova, Olesya; Prikhodko, Anna; Pinegina, Elena; Perelman, Juliy M.; Кolosov, Victor P.; Zhou, Xiangdong; Li, Qi

doi:10.1183/13993003.congress-2018.pa1272

Cited by 2 publications

(3 citation statements)

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“…Within bridge genes, MUC5AC is a glycoprotein and significantly increased when the respiratory tract was exposed to external stimulus [54]. High expression of MUC5AC was observed in bronchial epithelial cells of patients with severe asthma [55].…”

Section: Resultsmentioning

confidence: 99%

Consistent Biomarkers and Related Pathogenesis Underlying Asthma Revealed by Systems Biology Approach

Nie

Wei

Hao

et al. 2019

IJMS

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Asthma is a common chronic airway disease worldwide. Due to its clinical and genetic heterogeneity, the cellular and molecular processes in asthma are highly complex and relatively unknown. To discover novel biomarkers and the molecular mechanisms underlying asthma, several studies have been conducted by focusing on gene expression patterns in epithelium through microarray analysis. However, few robust specific biomarkers were identified and some inconsistent results were observed. Therefore, it is imperative to conduct a robust analysis to solve these problems. Herein, an integrated gene expression analysis of ten independent, publicly available microarray data of bronchial epithelial cells from 348 asthmatic patients and 208 healthy controls was performed. As a result, 78 up- and 75 down-regulated genes were identified in bronchial epithelium of asthmatics. Comprehensive functional enrichment and pathway analysis revealed that response to chemical stimulus, extracellular region, pathways in cancer, and arachidonic acid metabolism were the four most significantly enriched terms. In the protein-protein interaction network, three main communities associated with cytoskeleton, response to lipid, and regulation of response to stimulus were established, and the most highly ranked 6 hub genes (up-regulated CD44, KRT6A, CEACAM5, SERPINB2, and down-regulated LTF and MUC5B) were identified and should be considered as new biomarkers. Pathway cross-talk analysis highlights that signaling pathways mediated by IL-4/13 and transcription factor HIF-1α and FOXA1 play crucial roles in the pathogenesis of asthma. Interestingly, three chemicals, polyphenol catechin, antibiotic lomefloxacin, and natural alkaloid boldine, were predicted and may be potential drugs for asthma treatment. Taken together, our findings shed new light on the common molecular pathogenesis mechanisms of asthma and provide theoretical support for further clinical therapeutic studies.

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Section: Resultsmentioning

confidence: 99%

Consistent Biomarkers and Related Pathogenesis Underlying Asthma Revealed by Systems Biology Approach

Nie

Wei

Hao

et al. 2019

IJMS

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“…Menthol and inflammatory cytokines have been shown to increase airway mucins [ 17 , 23 , 24 ]. We, therefore, investigated the expression levels of the two major mucins found in the airways, namely MUC5AC and MUC5B [ 25 ].…”

Section: Resultsmentioning

confidence: 99%

“…Cold temperature and menthol were found to induce the expression of MUC5AC mRNA and protein in proliferating and submerged normal HBEC in a TRPM8-dependent manner [ 16 ]. Cold air was also shown to increase MUC5AC and decrease MUC5B mRNA expression in nasal cells of asthma patients [ 17 ]. It has been proposed that mucus concentration and the absolute concentrations of MUC5AC and MUC5B could be used as biomarkers of chronic bronchitis [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Nebulized Menthol Impairs Mucociliary Clearance via TRPM8 and MUC5AC/MUC5B in Primary Airway Epithelial Cells

Baumlin

Silswal²,

Dennis³

et al. 2023

IJMS

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Flavorings enhance the palatability of e-cigarettes (e-cigs), with menthol remaining a popular choice among e-cig users. Menthol flavor remains one of the only flavors approved by the United States FDA for use in commercially available, pod-based e-cigs. However, the safety of inhaled menthol at the high concentrations used in e-cigs remains unclear. Here, we tested the effects of menthol on parameters of mucociliary clearance (MCC) in air–liquid interface (ALI) cultures of primary airway epithelial cells. ALI cultures treated with basolateral menthol (1 mM) showed a significant decrease in ciliary beat frequency (CBF) and airway surface liquid (ASL) volumes after 24 h. Menthol nebulized onto the surface of ALI cultures similarly reduced CBF and increased mucus concentrations, resulting in decreased rates of mucociliary transport. Nebulized menthol further increased the expression of mucin 5AC (MUC5AC) and mRNA expression of the inflammatory cytokines IL1B and TNFA. Menthol activated TRPM8, and the effects of menthol on MCC and inflammation could be blocked by a specific TRPM8 antagonist. These data provide further evidence that menthol at the concentrations used in e-cigs could cause harm to the airways.

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Cold air alters MUC5AC and MUC5B expression in the airways of asthma patients

Cited by 2 publications

References 0 publications

Consistent Biomarkers and Related Pathogenesis Underlying Asthma Revealed by Systems Biology Approach

Consistent Biomarkers and Related Pathogenesis Underlying Asthma Revealed by Systems Biology Approach

Nebulized Menthol Impairs Mucociliary Clearance via TRPM8 and MUC5AC/MUC5B in Primary Airway Epithelial Cells

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