Cold and Heat Stress Diversely Alter Both Cauliflower Respiration and Distinct Mitochondrial Proteins Including OXPHOS Components and Matrix Enzymes

Abstract

Complex proteomic and physiological approaches for studying cold and heat stress responses in plant mitochondria are still limited. Variations in the mitochondrial proteome of cauliflower (Brassica oleracea var. botrytis) curds after cold and heat and after stress recovery were assayed by two-dimensional polyacrylamide gel electrophoresis (2D PAGE) in relation to mRNA abundance and respiratory parameters. Quantitative analysis of the mitochondrial proteome revealed numerous stress-affected protein spots. In cold, major downregulations in the level of photorespiratory enzymes, porine isoforms, oxidative phosphorylation (OXPHOS) and some low-abundant proteins were observed. In contrast, carbohydrate metabolism enzymes, heat-shock proteins, translation, protein import, and OXPHOS components were involved in heat response and recovery. Several transcriptomic and metabolic regulation mechanisms are also suggested. Cauliflower plants appeared less susceptible to heat; closed stomata in heat stress resulted in moderate photosynthetic, but only minor respiratory impairments, however, photosystem II performance was unaffected. Decreased photorespiration corresponded with proteomic alterations in cold. Our results show that cold and heat stress not only operate in diverse modes (exemplified by cold-specific accumulation of some heat shock proteins), but exert some associations at molecular and physiological levels. This implies a more complex model of action of investigated stresses on plant mitochondria.

Global identification and functional prediction of cold-related lncRNAs in eggplant

“…Regardless of regulatory manner, target genes of lncRNAs in both eggplant varieties were involved in plant hormone signal transduction, which was consistent with our finding in transcriptome analysis (Figure 5B, 5C, 6B, 6C) (Yang et al, 2020). It is widely reported that several pathways such as ubiquitin mediated proteolysis, starch and sucrose metabolism, oxidative phosphorylation and protein processing in endoplasmic reticulum were activated and respond to cold stress (Bar-Peled et al, 2007;Zhang et al, 2017;Rurek et al, 2018;Boulc et al, 2020). In our study, a majority of genes in cis-regulatory manner in both eggplant varieties took participate in those pathways (Figure 5B,5C), which indicated that lncRNAs could mediate eggplant cold response by cis-regulating these pathways.…”

Effects of Different Nitrogen Forms and Exogenous Application of Putrescine on Heat Stress of Cauliflower: Photosynthetic Gas Exchange, Mineral Concentration and Lipid Peroxidation

“…It is known that as a result of heat stress (depending on the intensity and duration), cauliflower can show wounds that range from a decrease in protein stability, or an increase in membrane fluidity, to the inactivation of mitochondrial enzymes [ 8 ]. Consequently, plants adjust their physiological and biochemical activities by altering their transcriptome, proteome, metabolome and lipidome to counteract these lesions and ensure cell survival [ 9 , 10 ].…”

Adaptation of broccoli (Brassica oleracea var. italica L.) to high and low altitudes in Bali, Indonesia

“…Heat stress limits plant growth, leaf development, photosynthesis activity, and yield on Brassica (Rodríguez et al 2015;Soengas et al 2018). Moreover, Rurek et al (2018) found that cauliflower closed stomata during heat stress, resulted in moderate photosynthetic with only minor respiratory damage. High temperature has also been reported to causing stress on the reproductive development of other Brassica species, i.e.…”