Cold Atmosphere Plasma Modification on Beta-Carotene-Loaded Nanofibers to Enhance Osteogenic Differentiation

Moradi, Yasamin; Atyabi, Seyed Alireza; Ghiassadin, Ali; Bakhshi, Hadi; Irani, Shiva; Atyabi, Seyed Mohammad; Dadgar, Neda

doi:10.1007/s12221-021-0033-y

Cited by 5 publications

(4 citation statements)

References 41 publications

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“…After 10 d of incubation, the cell viability for cells cultured on scaffolds PANi/PAN/CMC1%+βC, PANi/PAN/ CMC2%+βC, and PANi/PAN/CMC3%+βC was significantly decreased (p < 0.001), while the cells on scaffold PANi/PAN + βC and TCP continued to proliferate. The decrease in cell viability for scaffolds PANi/PAN/CMC1%+βC, PANi/PAN/CMC2%+βC, and PANi/PAN/ CMC3%+βC, amplified at higher surface-immobilized CMC contents, was not as a result of a cytotoxicity effect, but due to the switch from proliferation to differentiation [20,22,[25][26][27]. SEM images (Fig.…”

Section: Neural Differentiationmentioning

confidence: 92%

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Carboxymethyl Chitosan-Functionalized Polyaniline/Polyacrylonitrile Nano-Fibers for Neural Differentiation of Mesenchymal Stem Cells

Solimani

Irani

Mohamadali

et al. 2023

Appl Biochem Biotechnol

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Electroconductive scaffolds based on polyaniline (PANi)/polyacrylonitrile (PAN) were fabricated and surface-functionalized by carboxymethyl chitosan (CMC) as efficient scaffolds for nerve tissue regeneration. The results of scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, and water contact angle measurement approved the successful fabrication of CMC-functionalized PANi/PAN-based scaffolds. Human adipose-derived mesenchymal stem cells (hADMSCs) were cultured on the scaffolds for 10 d in the presence or absence of β-carotene (βC, 20 µM) as a natural neural differentiation agent. The MTT and SEM results confirmed the attachment and proliferation of hADMSCs on the scaffolds. The expression of MAP2 at the mRNA and protein levels showed the synergic neurogenic induction effect of CMC-functionalization and βC for hADMSCs on the scaffolds. The CMC-functionalized nanofibrous PANi/PAN-based scaffolds are potential candidates for nerve tissue engineering.

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Section: Neural Differentiationmentioning

confidence: 92%

“…Beta-Carotene (βC) is a plentiful provitamin A carotenoid found in dark green, yellow, and orange fruits and vegetables [20,[25][26][27]. Because of conjugated double bonds, βC has electrical activity [28,29].…”

Section: Introductionmentioning

confidence: 99%

Carboxymethyl Chitosan-Functionalized Polyaniline/Polyacrylonitrile Nano-Fibers for Neural Differentiation of Mesenchymal Stem Cells

Solimani

Irani

Mohamadali

et al. 2023

Appl Biochem Biotechnol

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“…Though exposing carboxyl groups may interfere with osteogenesis in vitro, studies have shown that negatively charged functional groups interact with calcium and phosphate ions present in bodily fluids and facilitate hydroxyapatite crystallization, which may aid in osteogenesis [157]. Interestingly, studies involving plasma treatment cite resultant exposure of carboxyl and hydroxyl groups as adhesion promoters, and thus osteogenesis promoters [158]. It is more probable that the hydrophilic effect from creating oxygen functional groups is what drives osteogenesis.…”

Section: Hydrophilic Surface Chemistry Improves Osteoconductivity Of ...mentioning

confidence: 99%

Mechanical Properties and Morphological Alterations in Fiber-Based Scaffolds Affecting Tissue Engineering Outcomes

Dolgin¹,

Hanumantharao

Farias³

et al. 2023

Fibers

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Electrospinning is a versatile tool used to produce highly customizable nonwoven nanofiber mats of various fiber diameters, pore sizes, and alignment. It is possible to create electrospun mats from synthetic polymers, biobased polymers, and combinations thereof. The post-processing of the end products can occur in many ways, such as cross-linking, enzyme linking, and thermal curing, to achieve enhanced chemical and physical properties. Such multi-factor tunability is very promising in applications such as tissue engineering, 3D organs/organoids, and cell differentiation. While the established methods involve the use of soluble small molecules, growth factors, stereolithography, and micro-patterning, electrospinning involves an inexpensive, labor un-intensive, and highly scalable approach to using environmental cues, to promote and guide cell proliferation, migration, and differentiation. By influencing cell morphology, mechanosensing, and intracellular communication, nanofibers can affect the fate of cells in a multitude of ways. Ultimately, nanofibers may have the potential to precisely form whole organs for tissue engineering, regenerative medicine, and cellular agriculture, as well as to create in vitro microenvironments. In this review, the focus will be on the mechanical and physical characteristics such as porosity, fiber diameter, crystallinity, mechanical strength, alignment, and topography of the nanofiber scaffolds, and the impact on cell proliferation, migration, and differentiation.

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“…PCL is frequently employed for bone and cartilage tissue engineering applications [ 8 , 22 ]. The major disadvantage of PCL is the lack of surface hydrophilicity/wettability, which influences the cell attachment and consequently their proliferation and differentiation, essential in tissue engineering [ 23 – 25 ]. Here, we immobilized CMC on the surface of PCL nanofibers through the chemical grafting reaction to simply fabricate novel scaffolds, which can induce osteochondral differentiation on stem cells without using any external differential agents.…”

Section: Introductionmentioning

confidence: 99%

Chemical Immobilization of Carboxymethyl Chitosan on Polycaprolactone Nanofibers as Osteochondral Scaffolds

Kabirkoohian

Bakhshi

Irani

et al. 2022

Appl Biochem Biotechnol

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Carboxymethyl chitosan (CMC) as a bio-based osteochondral inductive material was chemically immobilized on the surface of polycaprolactone (PCL) nanofibers to fabricate scaffolds for osteochondral tissue engineering applications. The chemical immobilization process included the aminolysis of ester bonds and bonding of the primary amines with glutaraldehyde as a coupling agent. The SEM and FTIR results confirmed the successfulness of the CMC immobilization. The fabricated scaffolds presented cell viabilities of > 82% and supported the attachment and proliferation of the human bone marrow mesenchymal stem cells (hBM-MSCs). The CMC-immobilized scaffolds concentration dependently induced the diverse osteochondral differentiation pathways for the hBM-MSCs without using any external differential agents. According to the Alcian Blue and Alizarin Red staining and immunocytochemistry results, scaffolds with a higher content of CMC presented more chondro-inductivity and less osteoinductivity. Thus, the CMC-immobilized scaffolds can be employed as great potential candidates for osteochondral tissue engineering applications.

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Cold Atmosphere Plasma Modification on Beta-Carotene-Loaded Nanofibers to Enhance Osteogenic Differentiation

Cited by 5 publications

References 41 publications

Carboxymethyl Chitosan-Functionalized Polyaniline/Polyacrylonitrile Nano-Fibers for Neural Differentiation of Mesenchymal Stem Cells

Carboxymethyl Chitosan-Functionalized Polyaniline/Polyacrylonitrile Nano-Fibers for Neural Differentiation of Mesenchymal Stem Cells

Mechanical Properties and Morphological Alterations in Fiber-Based Scaffolds Affecting Tissue Engineering Outcomes

Chemical Immobilization of Carboxymethyl Chitosan on Polycaprolactone Nanofibers as Osteochondral Scaffolds

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