Cold atmospheric plasma as a potential tool for multiple myeloma treatment

Xu, Dehui; Xu, Yujing; Cui, Qingjie; Liu, Dingxin; Liu, Zhijie; Wang, Xiaohua; Yang, Yanjie; Feng, Miaojuan; Liang, Rong; Chen, Hailan; Ye, Kai; Kong, Michael G.

doi:10.18632/oncotarget.24649

Cited by 32 publications

(26 citation statements)

References 62 publications

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“…Plasma-activated liquids display different biological actions which have been mainly attributed to the generation of RONS such as H 2 O 2 5,6 , NO 2 – 7 , ONOO − 8 , etc. These reactive species are known to be involved in a wide range of intracellular 6,7,9 and intercellular processes 10 and have shown a strong anti-cancer effect on several cancer cell lines in vitro 11–17 and on subcutaneously xenografted tumors in vivo 14,17–21 . Recently, proof of efficiency and selectivity in osteosarcoma was shown 10,22–24 .…”

Section: Introductionmentioning

confidence: 99%

Pyruvate Plays a Main Role in the Antitumoral Selectivity of Cold Atmospheric Plasma in Osteosarcoma

Tornín

Mateu-Sanz

Rodríguez

et al. 2019

Sci Rep

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Osteosarcoma (OS) is the most common primary bone tumor but current therapies still have poor prognosis. Cold Atmospheric Plasma (CAP) and Plasma activated media (PAM) have shown potential to eliminate cancer cells in other tumors. It is thought that Reactive Oxygen and Nitrogen species (RONS) in PAM are key players but cell culture media composition alters treatment outcomes and data interpretation due to scavenging of certain RONS. In this work, an atmospheric pressure plasma jet was employed to obtain PAM in the presence or absence of pyruvate and used to treat the SaOS-2 (OS) cell line or hBM-MSC healthy cells. OS cells show higher sensitivity to PAM treatment than healthy cells, both in medium with and without pyruvate, activating apoptosis, DNA damage and deregulating cellular pathways mediated by c-JUN, AKT, AMPK or STAT3. In line with previous works, lack of pyruvate increases cytotoxic potential of PAM affecting cancer and healthy cells by increasing 10–100 times the concentration of H 2 O 2 without altering that of nitrites and thus decreasing CAP anti-tumor selectivity. Suitable conditions for CAP anti-cancer selectivity can be obtained by modifying plasma process parameters (distance, flow, treatment time) to obtain adequate balance of the different RONS in cell culture media.

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Section: Introductionmentioning

confidence: 99%

Pyruvate Plays a Main Role in the Antitumoral Selectivity of Cold Atmospheric Plasma in Osteosarcoma

Tornín

Mateu-Sanz

Rodríguez

et al. 2019

Sci Rep

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“…These results might be due to the fact that SCC-15 cells respond to cisplatin treatment during the initial 6 h, and then apoptosis/necrosis is accelerated following cell death. Xu et al also reported that CAP increased caspase-9 activity of multiple myeloma and induced apoptosis/necrosis of cells [ 43 ]. Saadati et al also reported that indirect/direct treatment of B16F10 melanoma cells using CAP induces apoptosis-related protein expression, such as BAX/BCL-2 [ 44 ].…”

Section: Resultsmentioning

confidence: 99%

Combinatorial Effect of Cold Atmosphere Plasma (CAP) and the Anticancer Drug Cisplatin on Oral Squamous Cell Cancer Therapy

Lee

Jeong

Kook

et al. 2020

IJMS

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Cold atmospheric plasma (CAP) has been extensively investigated in the local treatment of cancer due to its potential of reactive oxygen species (ROS) generation in biological systems. In this study, we examined the synergistic effect of combination of CAP and cisplatin-mediated chemotherapy of oral squamous cell carcinoma (OSCC) in vitro. SCC-15 OSCC cells and human gingival fibroblasts (HGF-1) cells were treated with cisplatin, and then, the cells were irradiated with CAP. Following this, viability and apoptosis behavior of the cells were investigated. The viability of SCC-15 cells was inhibited by cisplatin with a dose-dependent manner and CAP treatment time. HGF-1 cells also showed decreased viability by treatment with cisplatin and CAP. Combination of 1 μM cisplatin plus 3 min of CAP treatment or 3 μM cisplatin plus 1 min of CAP treatment showed a synergistic anticancer effect with appropriate cytotoxicity against normal cells. ROS generation and dead cell staining were also increased by the increase in CAP treatment time. Furthermore, tumor-suppressor proteins and apoptosis-related enzymes also increased according to the treatment time of CAP. We showed the synergistic effect of cisplatin and CAP treatment against SCC-15 cells with low cytotoxicity against normal cells.

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“…Toxicological studies were CAP at 75kV, IC 50 was 0.0087 µg/ml. CAP similarly generates ROS and can be localised by selective application process at a milli or micro scale to cancer cells [50,51]. Consistent with the findings regarding to oxidative stress correlation to cell death with AgNP and CAP continuously mounts the evidence to show that generation of ROS is highly related to the mechanism of AgNP and CAP with the results in current study showed that the cytotoxicity induced by AgNP alone, as well as the combinational treatment AgNP-CAP were efficiently prevented prior NAC treatment up to 10-fold (see Figure 3).…”

Section: Discussionmentioning

confidence: 99%

Cold Atmospheric Plasma induces silver nanoparticle uptake, oxidative dissolution and enhanced cytotoxicity in Glioblastoma multiforme cells

Manaloto

Gowen²,

Leśniak³

et al. 2020

Preprint

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Running title: Cold Atmospheric Plasma enhances silver nanoparticle uptake and cytotoxicity.2 Abstract Silver nanoparticles (AgNP) emerged as a promising reagent for cancer therapy with oxidative stress implicated in the toxicity. Meanwhile, studies reported cold atmospheric plasma (CAP) generation of reactive oxygen and nitrogen species has selectivity towards cancer cells. Gold nanoparticles display synergistic cytotoxicity when combined with CAP against cancer cells but there is a paucity of information using AgNP, prompting to investigate the combined effects of CAP using dielectric barrier discharge system (voltage of 75 kV, current is 62.5mA, duty cycle of 7.5kVA and input frequency of 50-60Hz) and 10nm PVA-coated AgNP using U373MG Glioblastoma Multiforme cells. Cytotoxicity in U373MG cells was >100-fold greater when treated with both CAP and PVA-AgNP compared with either therapy alone (IC 50 of 4.30 µg/mL with PVA-AgNP alone compared with 0.07 µg/mL after 25s CAP and 0.01 µg/mL 40s CAP). Combined cytotoxicity was ROS-dependent and was prevented using N-Acetyl Cysteine. A novel darkfield spectral imaging method investigated and quantified AgNP uptake in cells determining significantly enhanced uptake, aggregation and subcellular accumulation following CAP treatment, which was confirmed and quantified using atomic absorption spectroscopy. The results indicate that CAP decreases nanoparticle size, decreases surface charge distribution of AgNP and induces uptake, aggregation and enhanced cytotoxicity in vitro.

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Cold atmospheric plasma as a potential tool for multiple myeloma treatment

Cited by 32 publications

References 62 publications

Pyruvate Plays a Main Role in the Antitumoral Selectivity of Cold Atmospheric Plasma in Osteosarcoma

Pyruvate Plays a Main Role in the Antitumoral Selectivity of Cold Atmospheric Plasma in Osteosarcoma

Combinatorial Effect of Cold Atmosphere Plasma (CAP) and the Anticancer Drug Cisplatin on Oral Squamous Cell Cancer Therapy

Cold Atmospheric Plasma induces silver nanoparticle uptake, oxidative dissolution and enhanced cytotoxicity in Glioblastoma multiforme cells

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