Cold Atmospheric Plasma (CAP) and CAP-Stimulated Cell Culture Media Suppress Ovarian Cancer Cell Growth – A Putative Treatment Option in Ovarian Cancer Therapy

Koensgen, Dominique; Besic, Ilma; Gümbel, Denis; Kaul, Anne; Weiß, Martin; Diesing, Karoline; Krämer, Axel; Bekeschus, Sander; Mustea, Alexander; Stope, Matthias B.

doi:10.21873/anticanres.12133

Cited by 37 publications

(30 citation statements)

References 30 publications

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“…Against this background, CAP treatment can be considered a new alternative in anti-oncological therapy. It has been shown that CAP treatment can inhibit both the proliferation and the cell mobility of in vitro propagated tumor cells [ 35 , 47 , 48 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

“…It is known that oxidative stress triggers apoptosis in epithelial cells [ 69 ]. Although CAP-induced apoptosis has been demonstrated in various tumor entities [ 34 , 35 , 70 , 71 ], the effect in endothelial cells is still largely unexplored. Analysis of apoptotic processes revealed both increased DNA fragmentation and increased activity of caspase 3 and caspase 7 in HDMEC directly treated with CAP.…”

Section: Discussionmentioning

confidence: 99%

“…The medical application of CAP has already been established in wound therapy, the sterilization of medical implants and devices, and the disinfection of air [ 30 , 31 ]. Another promising application of CAP is its use in the field of oncology, since an anti-proliferative effect of CAP on cancer cells of various cancers has already been demonstrated [ 32 , 33 , 34 , 35 ]. It is believed that, among other effects, the induction of apoptosis is the main reason for the anti-proliferative effect of CAP on cancer cells [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of Angiogenesis by Treatment with Cold Atmospheric Plasma as a Promising Therapeutic Approach in Oncology

Haralambiev

Neuffer

Nitsch

et al. 2020

IJMS

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Background: Cold atmospheric plasma (CAP) is increasingly used in the field of oncology. Many of the mechanisms of action of CAP, such as inhibiting proliferation, DNA breakage, or the destruction of cell membrane integrity, have been investigated in many different types of tumors. In this regard, data are available from both in vivo and in vitro studies. Not only the direct treatment of a tumor but also the influence on its blood supply play a decisive role in the success of the therapy and the patient’s further prognosis. Whether the CAP influences this process is unknown, and the first indications in this regard are addressed in this study. Methods: Two different devices, kINPen and MiniJet, were used as CAP sources. Human endothelial cell line HDMEC were treated directly and indirectly with CAP, and growth kinetics were performed. To indicate apoptotic processes, caspase-3/7 assay and TUNEL assay were used. The influence of CAP on cellular metabolism was examined using the MTT and glucose assay. After CAP exposure, tube formation assay was performed to examine the capillary tube formation abilities of HDMEC and their migration was messured in separate assays. To investigate in a possible mutagenic effect of CAP treatment, a hypoxanthine-guanine-phosphoribosyl-transferase assay with non malignant cell (CCL-93) line was performed. Results: The direct CAP treatment of the HDMEC showed a robust growth-inhibiting effect, but the indirect one did not. The MMT assay showed an apparent reduction in cell metabolism in the first 24 h after CAP treatment, which appeared to normalize 48 h and 72 h after CAP application. These results were also confirmed by the glucose assay. The caspase 3/7 assay and TUNEL assay showed a significant increase in apoptotic processes in the HDMEC after CAP treatment. These results were independent of the CAP device. Both the migration and tube formation of HDMEC were significant inhibited after CAP-treatment. No malignant effects could be demonstrated by the CAP treatment on a non-malignant cell line.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inhibition of Angiogenesis by Treatment with Cold Atmospheric Plasma as a Promising Therapeutic Approach in Oncology

Haralambiev

Neuffer

Nitsch

et al. 2020

IJMS

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“…At the same time, the potential of CAP in the treatment of gynaecological oncologic diseases can be illustrated by the example of current studies evaluating, for example, breast [55] or ovarian cancer [73]. There have not been any large clinical studies on CAP in gynaecological malignancies, however, although VSCC or cervical lesions are suitable for its use at large scale [69].…”

Section: Current Knowledge Of In Vitro Cell Lines and Further Potentimentioning

confidence: 99%

Cold Atmospheric Pressure Plasma (CAP) as a New Tool for the Management of Vulva Cancer and Vulvar Premalignant Lesions in Gynaecological Oncology

Žúbor

Wang

Líšková

et al. 2020

IJMS

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Vulvar cancer (VC) is a specific form of malignancy accounting for 5–6% of all gynaecologic malignancies. Although VC occurs most commonly in women after 60 years of age, disease incidence has risen progressively in premenopausal women in recent decades. VC demonstrates particular features requiring well-adapted therapeutic approaches to avoid potential treatment-related complications. Significant improvements in disease-free survival and overall survival rates for patients diagnosed with post-stage I disease have been achieved by implementing a combination therapy consisting of radical surgical resection, systemic chemotherapy and/or radiotherapy. Achieving local control remains challenging. However, mostly due to specific anatomical conditions, the need for comprehensive surgical reconstruction and frequent post-operative healing complications. Novel therapeutic tools better adapted to VC particularities are essential for improving individual outcomes. To this end, cold atmospheric plasma (CAP) treatment is a promising option for VC, and is particularly appropriate for the local treatment of dysplastic lesions, early intraepithelial cancer, and invasive tumours. In addition, CAP also helps reduce inflammatory complications and improve wound healing. The application of CAP may realise either directly or indirectly utilising nanoparticle technologies. CAP has demonstrated remarkable treatment benefits for several malignant conditions, and has created new medical fields, such as “plasma medicine” and “plasma oncology”. This article highlights the benefits of CAP for the treatment of VC, VC pre-stages, and postsurgical wound complications. There has not yet been a published report of CAP on vulvar cancer cells, and so this review summarises the progress made in gynaecological oncology and in other cancers, and promotes an important, understudied area for future research. The paradigm shift from reactive to predictive, preventive and personalised medical approaches in overall VC management is also considered.

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“…Cold atmospheric plasma (CAP) treatments have offered very promising opportunities for wound healing and antiseptics. Moreover, CAP indicated promising anti-neoplastic effects on several tumor entities, e.g., melanoma, glioma, pancreatic, and several gynecological tumors, particularly breast cancer, ovarian cancer, and cervical cancer [2][3][4][5][6][7][8][9][10][11][12][13]. CAP treatment led to sufficient inhibition of cancer cell growth, interestingly, without tissue swelling, inflammation, or pain.…”

Section: Introductionmentioning

confidence: 99%

Cancer-Selective Treatment of Cancerous and Non-Cancerous Human Cervical Cell Models by a Non-Thermally Operated Electrosurgical Argon Plasma Device

Feil

Koch

Utz

et al. 2020

Cancers

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Cold atmospheric plasma (CAP) treatment is developing as a promising option for local anti-neoplastic treatment of dysplastic lesions and early intraepithelial cancer. Currently, high-frequency electrosurgical argon plasma sources are available and well established for clinical use. In this study, we investigated the effects of treatment with a non-thermally operated electrosurgical argon plasma source, a Martin Argon Plasma Beamer System (MABS), on cell proliferation and metabolism of a tissue panel of human cervical cancer cell lines as well as on non-cancerous primary cells of the cervix uteri. Similar to conventional CAP sources, we were able to show that MABS was capable of causing antiproliferative and cytotoxic effects on cervical squamous cell and adenocarcinoma as well as on non-neoplastic cervical tissue cells due to the generation of reactive species. Notably, neoplastic cells were more sensitive to the MABS treatment, suggesting a promising new and non-invasive application for in vivo treatment of precancerous and cancerous cervical lesions with non-thermally operated electrosurgical argon plasma sources.

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Cold Atmospheric Plasma (CAP) and CAP-Stimulated Cell Culture Media Suppress Ovarian Cancer Cell Growth – A Putative Treatment Option in Ovarian Cancer Therapy

Abstract: In summary, the CAP application in oncological surgery leads to strong anti-proliferative effects and opens up novel opportunities for the OC treatment.

Cited by 37 publications

References 30 publications

Inhibition of Angiogenesis by Treatment with Cold Atmospheric Plasma as a Promising Therapeutic Approach in Oncology

Inhibition of Angiogenesis by Treatment with Cold Atmospheric Plasma as a Promising Therapeutic Approach in Oncology

Cold Atmospheric Pressure Plasma (CAP) as a New Tool for the Management of Vulva Cancer and Vulvar Premalignant Lesions in Gynaecological Oncology

Cancer-Selective Treatment of Cancerous and Non-Cancerous Human Cervical Cell Models by a Non-Thermally Operated Electrosurgical Argon Plasma Device

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