Colistin-Functionalized Nanoparticles for the Rapid Capture of <i>Acinetobacter baumannii</i>

Miller, Sinead; Bell, Charleson S; Mejías, Raquel; McClain, Mark S.; Cover, Timothy L.; Giorgio, Todd D.

doi:10.1166/jbn.2016.2273

Cited by 18 publications

(11 citation statements)

References 64 publications

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“…Therefore, there is an urgent need to identify and quantify the principal parameters governing the NPs interaction with the biological surfaces such as bacterial cell walls (Xing et al 2018). Most of the previous studies are focused on the interaction between NPs and bacteria, leading to an apoptotic disintegration of the bacterial cell walls (cells death) (Chwalibog et al 2010;Miller et al 2016;López-Lorente et al 2019). Yet, we believe that such knowledge may be used for designing microorganism-based effective systems, not only for the mere capture of NPs from the environment but also for the development of new functionalities associated with a controlled dispersion of NPs of various size, shape, and chemical nature (Polish Patents Applications P.426130 from 29 June 2018 and P.424760 from 5 March 2018).…”

Section: Introductionmentioning

confidence: 99%

Attachment efficiency of gold nanoparticles by Gram-positive and Gram-negative bacterial strains governed by surface charges

et al. 2019

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The interaction between metal nanoparticles and bacteria belongs to the central issues in a dynamically growing bionanotechnological research. Herein, we investigated the adhesion efficiency of gold nanoparticles (30 nm) for various bacterial strains, both Gram-positive (Bacillus subtilis, Staphylococcus carnosus) and Gram-negative (Neisseria subflava, Stenotrophomonas maltophilia). The thorough microscopic (SEM/TEM) observations revealed that the nanoparticles do not penetrate into the bacterial cells but adhere to the walls. Large differences in the adhered nanoparticles amount were observed for the investigated strains (B. subtilis >> S. carnosus > N. subflava > S. maltophilia). A direct correlation between the number of the attached nanoparticles and the ζ-potential of the bacterial strains was found, and the results were rationalized in terms of the DLVO model. The calculated DLVO energy profiles revealed that the activation barriers for the adhesion process are rather small (1.45-1.55 kT), and the primary energy minima of 120-170 kT are favorable for the effective adsorption process. The established linear correlation between the nanoparticles adhered to the cell surface and the size of the critical volume around the bacterial cell, where the attraction forces dominate, implies that the observed dramatic differences in the attachment efficiency result from the availability of the nanoparticles in the critical volume of the surrounding suspensions. Owing to non-specific interactions governed by the ζ-potential mainly, the obtained results can be readily extended for the other bacteria-nanoparticle systems, providing a rational background for future advances in bacteria detection and thorough characterization via SERS method as well as for nanoparticles assemblies towards nanoelectronics.

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Section: Introductionmentioning

confidence: 99%

Attachment efficiency of gold nanoparticles by Gram-positive and Gram-negative bacterial strains governed by surface charges

et al. 2019

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“…The following conditions were used: where r c was the effective spherical radius of a pathogen (0.5 μm, A . baumannii [ 54 ]; 0.65 μm, K . pneumoniae [ 55 ]) and it was assumed, based on previous literature, that half of the cell surface was covered by magnetic particles (ρ = 0.5 <dimensionless>)[ 13 , 53 ].…”

Section: Methodsmentioning

confidence: 99%

Dynamic Computational Model of Symptomatic Bacteremia to Inform Bacterial Separation Treatment Requirements

et al. 2016

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The rise of multi-drug resistance has decreased the effectiveness of antibiotics, which has led to increased mortality rates associated with symptomatic bacteremia, or bacterial sepsis. To combat decreasing antibiotic effectiveness, extracorporeal bacterial separation approaches have been proposed to capture and separate bacteria from blood. However, bacteremia is dynamic and involves host-pathogen interactions across various anatomical sites. We developed a mathematical model that quantitatively describes the kinetics of pathogenesis and progression of symptomatic bacteremia under various conditions, including bacterial separation therapy, to better understand disease mechanisms and quantitatively assess the biological impact of bacterial separation therapy. Model validity was tested against experimental data from published studies. This is the first multi-compartment model of symptomatic bacteremia in mammals that includes extracorporeal bacterial separation and antibiotic treatment, separately and in combination. The addition of an extracorporeal bacterial separation circuit reduced the predicted time of total bacteria clearance from the blood of an immunocompromised rodent by 49%, compared to antibiotic treatment alone. Implementation of bacterial separation therapy resulted in predicted multi-drug resistant bacterial clearance from the blood of a human in 97% less time than antibiotic treatment alone. The model also proposes a quantitative correlation between time-dependent bacterial load among tissues and bacteremia severity, analogous to the well-known ‘area under the curve’ for characterization of drug efficacy. The engineering-based mathematical model developed may be useful for informing the design of extracorporeal bacterial separation devices. This work enables the quantitative identification of the characteristics required of an extracorporeal bacteria separation device to provide biological benefit. These devices will potentially decrease the bacterial load in blood. Additionally, the devices may achieve bacterial separation rates that allow consequent acceleration of bacterial clearance in other tissues, inhibiting the progression of symptomatic bacteremia, including multi-drug resistant variations.

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“…The resulting particles had an average size of 20 nm and a surface charge of about −6 mV. In vitro studies showed that particle complexation with A. baumannii occurred rapidly and reached half-maximum saturation in 7 min [136].…”

Section: Conjugatesmentioning

confidence: 99%

Polymyxin Delivery Systems: Recent Advances and Challenges

Dubashynskaya

Skorik

2020

Pharmaceuticals

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Polymyxins are vital antibiotics for the treatment of multiresistant Gram-negative ESKAPE pathogen infections. However, their clinical value is limited by their high nephrotoxicity and neurotoxicity, as well as their poor permeability and absorption in the gastrointestinal tract. This review focuses on various polymyxin delivery systems that improve polymyxin bioavailability and reduce drug toxicity through targeted and controlled release. Currently, the most suitable systems for improving oral, inhalation, and parenteral polymyxin delivery are polymer particles, liposomes, and conjugates, while gels, polymer fibers, and membranes are attractive materials for topical administration of polymyxin for the treatment of infected wounds and burns. In general, the application of these systems protects polymyxin molecules from the negative effects of both physiological and pathological factors while achieving higher concentrations at the target site and reducing dosage and toxicity. Improving the properties of polymyxin will be of great interest to researchers who are focused on developing antimicrobial drugs that show increased efficacy and safety.

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Colistin-Functionalized Nanoparticles for the Rapid Capture of Acinetobacter baumannii

Cited by 18 publications

References 64 publications

Attachment efficiency of gold nanoparticles by Gram-positive and Gram-negative bacterial strains governed by surface charges

Attachment efficiency of gold nanoparticles by Gram-positive and Gram-negative bacterial strains governed by surface charges

Dynamic Computational Model of Symptomatic Bacteremia to Inform Bacterial Separation Treatment Requirements

Polymyxin Delivery Systems: Recent Advances and Challenges

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