Collagen Promotes Higher Adhesion, Survival and Proliferation of Mesenchymal Stem Cells

Chinnapaka, Somaiah; Kumar, Atul; Mawrie, Darilang; Sharma, Amit; Patil, Suraj Dasharath; Bhattacharyya, Jayanta; Swaminathan, Rajaram; Jaganathan, Bithiah Grace

doi:10.1371/journal.pone.0145068

Cited by 235 publications

(183 citation statements)

References 50 publications

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“…The optimum concentration of alginate used in our experiment caused the formation of suitable pore size, promoting the better supply of nutrients and cell transverse through shell membrane which was confirmed by SEM imaging (Yao et al, ). The existence of membrane pores in alginate‐gelatin microcapsules is importance for inward and outward diffusion of HUVECs and components in aqueous phase (Somaiah et al, ). Cell attachment to protein matrix per se induces signaling pathways, essential steps for the dynamic growth of cells.…”

Section: Discussionmentioning

confidence: 99%

Alginate‐gelatin encapsulation of human endothelial cells promoted angiogenesis in in vivo and in vitro milieu

Nemati

Rezabakhsh

Khoshfetrat

et al. 2017

Biotech & Bioengineering

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Up to present, many advantages have been achieved in the field of cell-based therapies by applying sophisticated methodologies and delivery approaches. Microcapsules are capable to provide safe microenvironment for cells during transplantation in a simulated physiological 3D milieu. Here, we aimed to investigate the effect of alginate-gelatin encapsulation on angiogenic behavior of human endothelial cells over a period of 5 days. Human umbilical vein endothelial cells were encapsulated by alginate-gelatin substrate and incubated for 5 days. MTT and autophagy PCR array analysis were used to monitor cell survival rate. For in vitro angiogenesis analysis, cell distribution of Tie-1, Tie-2, VEGFR-1, and VEGFR-2 were detected by ELISA. In addition to in vitro tubulogenesis assay, we monitored the expression of VE-cadherin by Western blotting. The migration capacity of encapsulated HUVECs was studied by measuring MMP-2 and MMP-9 via gelatin zymography. The in vivo angiogenic potential of encapsulated HUVECs was analyzed in immune-compromised mouse implant model during 7 days post-transplantation. We demonstrated that encapsulation promoted HUVECs cell survival and proliferation. Compared to control, no significant differences were observed in autophagic status of encapsulated cells (p > 0.05). The level of Tie-1, Tie-2, VEGFR-1, and VEGFR-2 were increased, but did not reach to significant levels. Encapsulation decreased MMP-2, -9 activity and increased the VE-cadherin level in enclosed cells (p < 0.05). Moreover, an enhanced in vivo angiogenic response of encapsulated HUVECs was evident as compared to non-capsulated cells (p < 0.05). These observations suggest that alginate-gelatin encapsulation can induce angiogenic response in in vivo and in vitro conditions.

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Section: Discussionmentioning

confidence: 99%

Alginate‐gelatin encapsulation of human endothelial cells promoted angiogenesis in in vivo and in vitro milieu

Nemati

Rezabakhsh

Khoshfetrat

et al. 2017

Biotech & Bioengineering

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“…These systems are convenient for downstream analyses, but lack the complexity of the native cellular milieu. To incorporate cell–ECM interactions within these platforms, TCP is often coated with purified ECM components (e.g., collagens, laminin, or fibronectin), which have been shown to mediate cell behavior . However, these simplified models lack the complex ultrastructure and biochemical composition of the native ECM, which may limit the translatability of the findings from in vitro to in vivo systems.…”

Section: Introductionmentioning

confidence: 99%

Culture on Tissue‐Specific Coatings Derived from α‐Amylase‐Digested Decellularized Adipose Tissue Enhances the Proliferation and Adipogenic Differentiation of Human Adipose‐Derived Stromal Cells

Shridhar

Lam

Sun

et al. 2019

Biotechnology Journal

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While extracellular matrix (ECM)‐derived coatings have the potential to direct the response of cell populations in culture, there is a need to investigate the effects of ECM sourcing and processing on substrate bioactivity. To develop improved cell culture models for studying adipogenesis, the current study examines the proliferation and adipogenic differentiation of human adipose‐derived stem/stromal cells (ASCs) on a range of ECM‐derived coatings. Human decellularized adipose tissue (DAT) and commercially available bovine tendon collagen (COL) are digested with α‐amylase or pepsin to prepare the coatings. Physical characterization demonstrates that α‐amylase digestion generates softer, thicker, and more stable coatings, with a fibrous tissue‐like ultrastructure that is lost in the pepsin‐digested thin films. ASCs cultured on the α‐amylase‐digested ECM have a more spindle‐shaped morphology, and proliferation is significantly enhanced on the α‐amylase‐digested DAT coatings. Further, the α‐amylase‐digested DAT provides a more pro‐adipogenic microenvironment, based on higher levels of adipogenic gene expression, glycerol‐3‐phosphate dehydrogenase (GPDH) enzyme activity, and perilipin staining. Overall, this study supports α‐amylase digestion as a new approach for generating bioactive ECM‐derived coatings, and demonstrates tissue‐specific bioactivity using adipose‐derived ECM to enhance ASC proliferation and adipogenic differentiation.

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“…This is mainly because the JBNT/FN Nano-Matrix are morphologically mimicking ECM, which F I G U R E 2 Demonstration for the self-assembled process of the Nano-matrix. Collagens have been shown to promote higher adhesion, survival and proliferation of MSCs (Somaiah et al, 2015). ECM determines tissue construction by providing a scaffold for cell adhesion (Singh & Schwarzbauer, 2012).…”

Section: Resultsmentioning

confidence: 99%

Self‐assembled biomimetic Nano‐Matrix for stem cell anchorage

Zhou

Yau

et al. 2020

J Biomedical Materials Res

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Mesenchymal stem cells (MSCs) have been widely applied in biomedicine due to their ability to differentiate into many different cell types and their ability to synthesize a broad spectrum of growth factors and cytokines that directly and indirectly influence other cells in their vicinity. To guide MSC infiltration to a bone fracture site, we developed a novel self-assembled Nano-Matrix which can be used as an injectable scaffold to repair bone fractures. The Nano-Matrix is formed by Janus base nanotubes (JBNTs) and fibronectin (FN). JBNTs are nucleobase-derived nanotubes mimicking collagen fibers, and FN is one of the cell adhesive glycoproteins which is responsible for cell-extracellular matrix interactions and guides stem cell migration and differentiation to desired cells types. Here, we demonstrated the successful fabrication and characterization of the JBNT/FN Nano-Matrix as well as its excellent bioactivity that encouraged human MSC migration and adhesion. This work lays a solid foundation for using the Nano-Matrix as an injectable approach to improve MSC retention and function during bone fracture healing. K E Y W O R D Sanchorage, fibronectin, Janus-based nanotubes, mesenchymal stem cells, Nano-Matrix

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Collagen Promotes Higher Adhesion, Survival and Proliferation of Mesenchymal Stem Cells

Cited by 235 publications

References 50 publications

Alginate‐gelatin encapsulation of human endothelial cells promoted angiogenesis in in vivo and in vitro milieu

Alginate‐gelatin encapsulation of human endothelial cells promoted angiogenesis in in vivo and in vitro milieu

Culture on Tissue‐Specific Coatings Derived from α‐Amylase‐Digested Decellularized Adipose Tissue Enhances the Proliferation and Adipogenic Differentiation of Human Adipose‐Derived Stromal Cells

Self‐assembled biomimetic Nano‐Matrix for stem cell anchorage

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