Collagen secretion by clonal and primary human muscle cells in culture

Partial characterization of a low-molecular-weight collagen synthesized by chondrocytes cultured in collagen gels

“…The media of the cultures were separated from the cell layers, and the collagens were extracted from the medium as previously described (Kundu et al, 1981), except where the collagens were precipitated with 1.2~-instead of 0.9 M (Ristell et al, 1980). The collagens were separated by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis (Sykes et al, 1976).…”

“…The collagens were separated by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis (Sykes et al, 1976). The proportions of the various collagens were determined from the amount of radioactivity present in 1 mm gel slices (Kundu et al, 1981). As an index of cell density we measured the protein content of the cell layer by the Lowry method.…”