Collagen structures in pericardium and aortic heart valves and their significance for tissue engineering

Filová, Elena; Burdíková, Zuzana; Staňková, Lubica; Hadraba, Daniel; Švindrych, Zdeněk; Schornik, David; Bačáková, Lucie; Chlup, Hynek; Gultova, Eva; Vesely, Jan; Horny, Lukas; Žitný, Rudolf; Straka, Frantisek; Pirk, Jan

doi:10.1109/ehb.2013.6707382

Cited by 7 publications

(6 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast to the analyzed jellyfish collagen scaffolds, the porcine pericardium has a special collagen fiber orientation due to its special functionality as a dominant load bearing component [59]. Moreover, the mammalian pericardium contains highly organized collagen fibers and bears a higher extent of natural crosslinking based on a higher glycosaminoglycan (GAG) content which makes this tissue denser compared to other tissues [60,61]. Altogether, it is assumable that both the collagen composition and also the crosslinking treatment of the jellyfish collagen scaffolds could be the basis for the observed faster degradation in concert with the occurrence of multinucleated giant cells, which are fused macrophages that are supposed to have a higher phagocytosis capacity [62,63].…”

Section: Discussionmentioning

confidence: 99%

In Vivo Analysis of the Biocompatibility and Immune Response of Jellyfish Collagen Scaffolds and its Suitability for Bone Regeneration

Flaig¹,

Radenković

Najman

et al. 2020

IJMS

View full text Add to dashboard Cite

Jellyfish collagen, which can be defined as “collagen type 0” due to its homogeneity to the mammalian types I, II, III, V, and IX and its batch-to-batch consistent producibility, is of special interest for different medical applications related to (bone) tissue regeneration as an alternative to mammalian collagen-based biomaterials. However, no in vivo studies regarding the induction of M1- and M2-macrophages and their time-dependent ration as well as the analysis of the bone regeneration capacity of jellyfish collagen scaffolds have been conducted until now. Thus, the goal of this study was to determine the nature of the immune response to jellyfish collagen scaffolds and their bone healing capacities. Two in vivo studies using established implantation models, i.e., the subcutaneous and the calvarian implantation model in Wistar rats, were conducted. Furthermore, specialized histological, histopathological, and histomorphometrical methods have been used. As a control biomaterial, a collagen scaffold, originating from porcine pericardium, which has already been stated as biocompatible, was used for the subcutaneous study. The results of the present study show that jellyfish collagen scaffolds are nearly completely resorbed until day 60 post implantation by stepwise integration within the subcutaneous connective tissue mediated mainly by macrophages and single multinucleated giant cells. Interestingly, the degradation process ended in a vessel rich connective tissue that is understood to be an optimal basis for tissue regeneration. The study results showed an overall weaker immune response to jellyfish collagen than to porcine pericardium matrices by the induction of significantly lower numbers of macrophages together with a more balanced occurrence of M1- and M2-macrophages. However, both collagen-based biomaterials induced balanced numbers of both macrophage subtypes, which supports their good biocompatibility. Moreover, the histomorphometrical results for the calvarial implantation of the jellyfish scaffolds revealed an average of 46.20% de novo bone formation at day 60, which was significantly higher compared to the control group. Thereby, the jellyfish collagen scaffolds induced also significantly higher numbers of anti-inflammatory macrophages within the bony implantation beds. Altogether, the results show that the jellyfish collagen scaffolds allowed for a directed integration behavior, which is assumed to be in accordance with the concept of Guided Bone Regeneration (GBR). Furthermore, the jellyfish collagen scaffolds induced a long-term anti-inflammatory macrophage response and an optimal vascularization pattern within their implant beds, thus showing excellent biocompatibility and (bone) tissue healing properties.

show abstract

Section: Discussionmentioning

confidence: 99%

In Vivo Analysis of the Biocompatibility and Immune Response of Jellyfish Collagen Scaffolds and its Suitability for Bone Regeneration

Flaig¹,

Radenković

Najman

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…The experience with biological valves in rheumatic diseases is similarly unsatisfactory, with early degeneration and an increased risk of reoperation [13] . Histological differences in pericardial and valve tissue composition may reduce the risk of future degeneration of the aortic autopericardial cusps [14] . The use of homografts and reconstructive surgeries using autologous materials allow not only avoiding lifelong intake of anticoagulants, but also maintaining hemodynamic profile comparable to that of native valves.…”

Section: Discussionmentioning

confidence: 99%

Case report: Autopericardial aortic valve replacement and mitral homograft implantation in a patient with ankylosing spondylitis

Комаров

Tkachev

Ismailbaev

et al. 2023

International Journal of Surgery Case Reports

View full text Add to dashboard Cite

“…Assessments were conducted utilizing porcine pericardium, the use of which is justified as the amount of collagen and fiber organization is similar to human pericardial tissue [35].…”

Section: Discussionmentioning

confidence: 99%

A Glutaraldehyde-Free Crosslinking Method for the Treatment of Collagen-Based Biomaterials for Clinical Application

Steitz,

Zouhair,

Khan

et al. 2023

Bioengineering

View full text Add to dashboard Cite

Biological bioprostheses such as grafts, patches, and heart valves are often derived from biological tissue like the pericardium. These bioprostheses can be of xenogenic, allogeneic, or autologous origin. Irrespective of their origin, all types are pre-treated via crosslinking to render the tissue non-antigenic and mechanically strong or to minimize degradation. The most widely used crosslinking agent is glutaraldehyde. However, glutaraldehyde-treated tissue is prone to calcification, inflammatory degradation, and mechanical injury, and it is incapable of matrix regeneration, leading to structural degeneration over time. In this work, we are investigating an alternative crosslinking method for an intraoperative application. The treated tissue‘s crosslinking degree was evaluated by differential scanning calorimetry. To confirm the findings, a collagenase assay was conducted. Uniaxial tensile testing was used to assess the tissue’s mechanical properties. To support the findings, the treated tissue was visualized using two-photon microscopy. Additionally, fourier transform infrared spectroscopy was performed to study the overall protein secondary structure. Finally, a crosslinking procedure was identified for intraoperative processing. The samples showed a significant increase in thermal and enzymatic stability after treatment compared to the control, with a difference of up to 22.2 °C and 100%, respectively. Also, the tissue showed similar biomechanics to glutaraldehyde-treated tissue, showing greater extensibility, a higher failure strain, and a lower ultimate tensile strength than the control. The significant difference in the structure band ratio after treatment is proof of the introduction of additional crosslinks compared to the untreated control with regard to differences in the amide-I region. The microscopic images support these findings, showing an alteration of the fiber orientation after treatment. For collagen-based biomaterials, such as pericardial tissue, the novel phenolic crosslinking agent proved to be an equivalent alternative to glutaraldehyde regarding tissue characteristics. Although long-term studies must be performed to investigate superiority in terms of longevity and calcification, our novel crosslinking agent can be applied in concentrations of 1.5% or 2.0% for the treatment of biomaterials.

show abstract

Collagen structures in pericardium and aortic heart valves and their significance for tissue engineering

Cited by 7 publications

References 5 publications

In Vivo Analysis of the Biocompatibility and Immune Response of Jellyfish Collagen Scaffolds and its Suitability for Bone Regeneration

In Vivo Analysis of the Biocompatibility and Immune Response of Jellyfish Collagen Scaffolds and its Suitability for Bone Regeneration

Case report: Autopericardial aortic valve replacement and mitral homograft implantation in a patient with ankylosing spondylitis

A Glutaraldehyde-Free Crosslinking Method for the Treatment of Collagen-Based Biomaterials for Clinical Application

Contact Info

Product

Resources

About