Collagen type I-based recombinant peptide promotes bone regeneration in rat critical-size calvarial defects by enhancing osteoclast activity at late stages of healing

Chimedtseren, Ichinnorov; Yamahara, Shoji; Akiyama, Yasunori; Ito, Masaaki; Arai, Yoshinori; Gantugs, Anar Erdene; Nastume, Nagato; Wakita, Taku; Hiratsuka, Takahiro; Honda, Masaki; Montenegro Raudales, Jorge Luis

doi:10.1016/j.reth.2023.09.013

Cited by 3 publications

(1 citation statement)

References 47 publications

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“…Moreover, the osteogenic capacity of rib bone grafts may be comparatively lower than that of iliac grafts due to a relatively lower proportion of cancellous bone and a corresponding scarcity of osteoinductive factors, including bone morphogenetic proteins (BMPs) and growth factors [ 42 – 44 ]. Conversely, the iliac crest, with its abundance of cancellous bone, serves as a robust source of osteoprogenitor cells and vascular marrow, facilitating superior osteoinduction and a vigorous osteogenic response after implantation [ 40 , 45 – 48 ]. Its denser cortical layer imparts enhanced resistance to compression, which is crucial for maintaining the stability of the anterior and middle columns post-surgery.…”

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of multi-fold rib and structural iliac bone grafts in single-segment thoracic and thoracolumbar spinal tuberculosis: clinical and radiological outcomes

Luo,

Chen,

Xiu

et al. 2023

J Orthop Surg Res

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Objective To compare clinical and radiological outcomes of multi-fold rib and structural iliac bone grafts, the primary autologous graft techniques in anterolateral-only surgery for single-segment thoracic and thoracolumbar spinal tuberculosis. Methods This retrospective study included 99 patients treated from January 2014 to March 2022, categorized into 64 with multi-fold rib grafts (group A) and 35 with structural iliac bone grafts (group B). Outcomes assessed included hospital stay, operation time, intraoperative blood loss, postoperative drainage, complications, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), the Visual Analog Scale (VAS) for pain, the Oswestry Disability Index (ODI), bone fusion time, and the American Spinal Injury Association (ASIA) impairment scale grade. Segmental kyphotic angle and intervertebral height were measured radiologically before surgery and follow-up. Results The mean follow-up was 63.50 ± 26.05 months for group A and 64.97 ± 26.43 months for group B (P > 0.05). All patients had achieved a clinical cure. Group A had a shorter operation time (P = 0.004). Within one week post-surgery, group B reported higher VAS scores (P < 0.0001). Neurological performance and quality of life significantly improved in both groups. No significant differences were observed in segmental kyphotic angle and intervertebral height between the groups pre- and postoperatively (P > 0.05). However, group A showed a greater segmental kyphotic angle at the final follow-up, while group B had better maintenance of kyphotic angle correction and intervertebral height (P < 0.05). Bone fusion was achieved in all patients without differences in fusion time (P > 0.05). Conclusions Multi-fold rib grafts resulted in shorter operation times and less postoperative pain, while structural iliac bone grafts provided better long-term maintenance of spinal alignment and stability, suggesting their use in cases where long-term outcomes are critical.

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Section: Discussionmentioning

confidence: 99%

Comparative evaluation of multi-fold rib and structural iliac bone grafts in single-segment thoracic and thoracolumbar spinal tuberculosis: clinical and radiological outcomes

Luo,

Chen,

Xiu

et al. 2023

J Orthop Surg Res

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Biomaterial Cues for Regulation of Osteoclast Differentiation and Function in Bone Regeneration

Shariati,

Bedar,

Huang

et al. 2024

Advanced Therapeutics

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Tissue regeneration involves dynamic dialogue between and among different cells and their surrounding matrices. Bone regeneration is specifically governed by reciprocity between osteoblasts and osteoclasts within the bone microenvironment. Osteoclast‐directed resorption and osteoblast‐directed formation of bone are essential to bone remodeling, and the crosstalk between these cells is vital to curating a sequence of events that culminate in the creation of bone tissue. Among bone biomaterial strategies, many have investigated the use of different material cues to direct the development and activity of osteoblasts. However, less attention has been given to exploring features that similarly target osteoclast formation and activity, with even fewer strategies demonstrating or integrating biomaterial‐directed modulation of osteoblast‐osteoclast coupling. This review aims to describe various biomaterial cues demonstrated to influence osteoclastogenesis and osteoclast function, emphasizing those that enhance a material construct's ability to achieve bone healing and regeneration. Additionally discussed are approaches that influence the communication between osteoclasts and osteoblasts, particularly in a manner that takes advantage of their coupling. Deepening the understanding of how biomaterial cues may dictate osteoclast differentiation, function, and influence on the microenvironment may enable the realization of bone‐replacement interventions with enhanced integrative and regenerative capacities.

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Biofunctional Characterization of Collagen from Orange-Spotted Grouper, Epinephelus coioides: Maintenance of Cartilage in Aged Zebrafish

Hu,

Tsou,

Tseng

et al. 2024

Fishes

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In this investigation, collagen was successfully extracted from the skin and fins of the orange-spotted grouper, Epinephelus coioides, with recovery rates of 4.45% and 23.65% (dry weight basis), respectively. UV–Vis spectrophotometric analysis demonstrated distinct absorbance peaks at 222 nm and 217 nm for collagen isolated from the skin and fins, correspondingly. Both collagen sources were confirmed to be type I, characterized by the presence of two α-chains (α1 and α2), with glycine as the predominant amino acid, an absence of tryptophan, and a notable content of proline and hydroxyproline. The enzymatic hydrolysis of fin-derived collagen using pepsin yielded low-molecular-weight peptides (PHC), which were subsequently incorporated into the diet of 1.5-year-old zebrafish, either alone or in combination with glucosamine (GC), to assess their biological effects. After eight weeks of dietary supplementation, zebrafish fed PHC or the combined PHC + GC diets exhibited a significant upregulation of sox9a (jef) expression in spinal tissues, accompanied by a marked downregulation of runx2. Although the differences in swimming performance among the groups were not statistically significant, zebrafish that received PHC or the combined supplements demonstrated enhanced endurance compared to the control group. These results suggest that collagen sourced from grouper may have advantageous effects in supporting cartilage health in aged zebrafish. Furthermore, utilizing fish by-products for collagen extraction enhances resource efficiency and aligns with circular economy principles.

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Collagen type I-based recombinant peptide promotes bone regeneration in rat critical-size calvarial defects by enhancing osteoclast activity at late stages of healing

Cited by 3 publications

References 47 publications

Comparative evaluation of multi-fold rib and structural iliac bone grafts in single-segment thoracic and thoracolumbar spinal tuberculosis: clinical and radiological outcomes

Comparative evaluation of multi-fold rib and structural iliac bone grafts in single-segment thoracic and thoracolumbar spinal tuberculosis: clinical and radiological outcomes

Biomaterial Cues for Regulation of Osteoclast Differentiation and Function in Bone Regeneration

Biofunctional Characterization of Collagen from Orange-Spotted Grouper, Epinephelus coioides: Maintenance of Cartilage in Aged Zebrafish

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