Collagen type VIII alpha 2 chain (COL8A2), an important component of the basement membrane of the corneal endothelium, facilitates the malignant development of glioblastoma cells via inducing EMT

Cheng, Ying-xin; Xiao, Lin; Yang, Yan-Li; Liu, Xiaodong; Zhou, Xiu-Rong; Bu, Zhenfu; Cao, Peicheng; Wang, Dao-kui

doi:10.1007/s10863-020-09865-1

Cited by 15 publications

(7 citation statements)

References 35 publications

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“…However, if we adopt the definitions of Jakus (1956) and Murphy et al (1984) , all of the vertebrates with thin, incomplete, irregular collections of loosely-woven fine fibrils would be better described as having a basement membrane rather than a Desçemet’s membrane and this includes the lampreys. Further differentiation may be possible as a result of tissue analysis of the membranes, e.g., using monoclonal antibodies to label components such as collagen ( Cheng et al, 2021 ) and laminin ( Leung et al, 2000 ). Collagen Type IV and laminin are major components of basement membranes ( Kefalides et al, 1979 ; Kühn et al, 1981 ; Sanes et al, 1990 ), while Desçemet’s membrane, a morphologically unique basement membrane, is rich in collagen Type VIII ( Kapoor et al, 1986 ; Illidge et al, 2001 ), in addition to some collagen Type IV ( Leung et al, 2000 ), although, in rabbits, Desçemet’s membrane does not contain laminin ( Leung et al, 2000 ).…”

Section: Discussionmentioning

confidence: 99%

The Functional Anatomy of the Cornea and Anterior Chamber in Lampreys: Insights From the Pouched Lamprey, Geotria australis (Geotriidae, Agnatha)

2021

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Extant lampreys (Petromyzontiformes) are one of two lineages of surviving jawless fishes or agnathans, and are therefore of critical importance to our understanding of vertebrate evolution. Anadromous lampreys undergo a protracted lifecycle, which includes metamorphosis from a larval ammocoete stage to an adult that moves between freshwater and saltwater with exposure to a range of lighting conditions. Previous studies have revealed that photoreception differs radically across the three extant families with the Pouched lamprey Geotria australis possessing a complex retina with the potential for pentachromacy. This study investigates the functional morphology of the cornea and anterior chamber of G. australis, which is specialised compared to its northern hemisphere counterparts. Using light microscopy, scanning and transmission electron microscopy and microcomputed tomography, the cornea is found to be split into a primary spectacle (dermal cornea) and a scleral cornea (continuous with the scleral eyecup), separated by a mucoid layer bounded on each side by a basement membrane. A number of other specialisations are described including mucin-secreting epithelial cells and microholes, four types of stromal sutures for the inhibition of stromal swelling, abundant anastomosing and branching of collagen lamellae, and a scleral endothelium bounded by basement membranes. The structure and function of the cornea including an annular and possibly a pectinate ligament and iris are discussed in the context of the evolution of the eye in vertebrates.

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Section: Discussionmentioning

confidence: 99%

The Functional Anatomy of the Cornea and Anterior Chamber in Lampreys: Insights From the Pouched Lamprey, Geotria australis (Geotriidae, Agnatha)

2021

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“…The alpha 2 chain of type VIII collagen is encoded by COL8A2, which possessed two non-triple helical domains at the N and C termini and a brief triple-helical domain. It was demonstrated that knockdown of COL8A2 inhibited the proliferation, migration, and invasion of GBM cells via downregulating the expression of EMT-related proteins in GBM (Cheng et al 2021). Claudin-10, encoded by CLDN10, was expressed broadly in the kidney, skin, and salivary glands and served as an integral tight junction membrane-spanning protein in two alternatively spliced variant forms(Hadj-Rabia et al 2018).…”

Section: Discussionmentioning

confidence: 99%

Identification of novel gene signature predicting lymph node metastasis in papillary thyroid cancer via bioinformatics analysis and in vitro validation

li,

Sun,

Jin

et al. 2024

Preprint

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Background Although with a good prognosis of papillary thyroid cancer (PTC), the patients in PTC experiencing lymph node metastasis (LNM) remained higher recurrence and mortality rate. It was still essential to explore novel biomarkers or methods to predict and evaluate the situation in the stages of PTC. Method In this study, mRNA sequence datasets from The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO) were utilized to obtain differentially expressed genes (DEGs) between PTC tumor and normal specimens and DEGs related to lymph node metastasis were identified using Weighted Gene Co-expression Network Analysis (WGCNA) according to the clinical information. Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were applied to quest the biological functions and pathways. Furthermore, protein-protein interaction (PPI) network was constructed using STRING database and a prognosis model was established using the least absolute shrinkage and selection operator (LASSO) Cox regression analysis based on the LNM-related DEGs. Finally, six hub genes were identified and verified in vitro experiments. Results LNM-related co-expression modules were identified using WGCNA analysis from samples of TCGA THCA and GSE60542. A novel six-gene signature model including COL8A2, MET, FN1, MPZL2, PDLIM4 and CLDN10 was established based on totally 52 DEGs from the intersection of those modules to predict the situation of lymph node metastasis in PTC. Those six hub genes were all higher expressed in PTC tumors and played potential biological functions on the development of PTC in vitro experiments, which had potential values as diagnostic and therapeutic targets.

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“…COL8A1 is also upregulated in colon and gastric cancers and expression correlated with progression and poor survival [57][58][59]. Similarly, COL8A2 is upregulated and associated with poor survival in glioblastoma [60]. Type VIII collagen also has a matrikine in its C-terminal NC1 domain, called Vastatin, that reportedly has anti-angiogenic properties [61,62], yet is elevated in the serum of colorectal patients [63].…”

Section: Discussionmentioning

confidence: 99%

The collagen landscape in cancer: profiling collagens in tumors and in circulation reveals novel markers of cancer‐associated fibroblast subtypes

Thorlacius‐Ussing,

Jensen,

Nissen

et al. 2023

The Journal of Pathology

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Cancer‐associated fibroblasts (CAFs) deposit and remodel collagens in the tumor stroma, impacting cancer progression and efficacy of interventions. CAFs are the focus of new therapeutics with the aim of normalizing the tumor microenvironment. To do this, a better understanding of CAF heterogeneity and collagen composition in cancer is needed. In this study, we sought to profile the expression of collagens at multiple levels with the goal of identifying cancer biomarkers. We investigated the collagen expression pattern in various cell types and CAF subtypes in a publicly available single‐cell RNA sequencing (RNA‐seq) dataset of pancreatic ductal adenocarcinoma. Next, we investigated the collagen expression profile in tumor samples across cancer types from The Cancer Genome Atlas (TCGA) database and evaluated if specific patterns of collagen expression were associated with prognosis. Finally, we profiled circulating collagen peptides using a panel of immunoassays to measure collagen fragments in the serum of cancer patients. We found that pancreatic stellate cells and fibroblasts were the primary producers of collagens in the pancreas. COL1A1, COL3A1, COL5A1, COL6A1 were expressed in all CAF subtypes, whereas COL8A1, COL10A1, COL11A1, COL12A1 were specific to myofibroblast CAFs (myCAF) and COL14A1 specific to inflammatory CAFs (iCAF). In TCGA database, myCAF collagens COL10A1 and COL11A1 were elevated across solid tumor types, and multiple associations between high expression and worse survival were found. Finally, circulating collagen biomarkers were elevated in the serum of patients with cancer relative to healthy controls with COL11A1 (myCAF) having the best diagnostic accuracy of the markers measured. In conclusion, CAFs express a noncanonical collagen profile with specific collagen subtypes associated with iCAFs and myCAFs in PDAC. These collagens are deregulated at the cellular, tumor, and systemic levels across different solid tumors and associate with survival. These findings could lead to new discoveries such as novel biomarkers and therapeutic targets. © 2023 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.

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Collagen type VIII alpha 2 chain (COL8A2), an important component of the basement membrane of the corneal endothelium, facilitates the malignant development of glioblastoma cells via inducing EMT

Cited by 15 publications

References 35 publications

The Functional Anatomy of the Cornea and Anterior Chamber in Lampreys: Insights From the Pouched Lamprey, Geotria australis (Geotriidae, Agnatha)

The Functional Anatomy of the Cornea and Anterior Chamber in Lampreys: Insights From the Pouched Lamprey, Geotria australis (Geotriidae, Agnatha)

Identification of novel gene signature predicting lymph node metastasis in papillary thyroid cancer via bioinformatics analysis and in vitro validation

The collagen landscape in cancer: profiling collagens in tumors and in circulation reveals novel markers of cancer‐associated fibroblast subtypes

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