In this study, eight cultivars, all of which are Vitis vinifera L., are grown in Aksaray province were determined. Identification material was selected approximately 20 years old, own roots vines in the producer vineyards. Ampelographic characteristics are defined by 123 descriptive characters in the OIV (The International Organization of Vine and Wine) list of grape and grapevine rootstock varieties identification criteria. With the numerical codes of the OIV identification criteria, similarity analysis was made in the IBM SPSS Statistics software (SPSS) package program, and the similarity relationships of the cultivars were visualized with a dendrogram. Aşeri and Mor Üzüm varieties defined in Aksaray province are not registered in the European Catalogue, but all varieties are Vitis vinifera L. Subsp. vinifera is registered as Turkey varieties. All the cultivar excluding Aşeri, and Emir names defined here are included in the Turkey grapevine genetic resources list, and several cultivars containing the same name or synonyms have been defined in different studies. The ampelographic descriptions of Aşeri and Emir grape varieties were made for the first time. The ampelographic definitions of Ak Dimrit, Çavuş, Kalecik Karası, Mor Üzüm, Parmak Üzümü and Sergi Karası cvs were confirmed with previous descriptions made in different provinces. The similarities and differences of the varieties we identified were reflected in the similarity dendrogram. Six of the eight grape varieties are hermaphrodite, and two of them (Çavuş and Sergi Karası) are functional female flowers. In the international variety catalogue records, the most common Aksaray variety on a global scale is Çavuş variety originating from Turkey. The cv. Parmak Üzümü was a separate ecotype due to its differences from previous identification records. It would be appropriate to protect the Aşeri, Emir, and Parmak Üzümü varieties, which we described for the first time, in the grapevine genetic resources of Turkey