Background: The Susan G. Komen for the Cure Tissue Bank at the IU Simon Cancer Center (KTB) was established in 2007 with funding from Susan G. Komen for the Cure to provide scientists with a resource for normal breast tissue. To date, nearly 3,500 women have donated their healthy breast tissue to the bank, but little is known about their perspectives. This study was designed to examine their motivations, concerns, and experiences.Methods: We conducted brief interviews with donors (n ¼ 221) to investigate their donation-related motivations, concerns, and experiences. Donor responses were coded and quantitatively analyzed (descriptive statistics and c 2 ). Results: The most frequent motivation to donate (48% of donors) was personal connection to a breast cancer patient/ survivor. A majority of donors (60%) were unconcerned about donation before the event; reported concerns included pain,