Colloidal gold-based immunochromatographic assay for detection of botulinum neurotoxin type B

Chiao, Der-Jiang; Shyu, Rong-Hwa; Hu, Chih-Fen; Chiang, Hsien-Yuan; Tang, Shiao-Shek

doi:10.1016/j.jchromb.2004.05.033

Cited by 117 publications

(61 citation statements)

References 13 publications

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“…An ICA was developed for the detection of anti-CHIKV capsid protein IgM using the method described previously (Chiao et al, 2004;Kim et al, 2007;Shyu et al, 2002). In brief, colloidal gold (40 nm in diameter, British Biocell International, UK) was used for the conjugation of anti-human IgM.…”

Section: Immunochromatographic Assay (Ica)mentioning

confidence: 99%

Expression of the capsid protein of Chikungunya virus in a baculovirus for serodiagnosis of Chikungunya disease

Cho¹,

Kim²,

Cho

et al. 2008

Journal of Virological Methods

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Section: Immunochromatographic Assay (Ica)mentioning

confidence: 99%

Expression of the capsid protein of Chikungunya virus in a baculovirus for serodiagnosis of Chikungunya disease

Cho¹,

Kim²,

Cho

et al. 2008

Journal of Virological Methods

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“…The antibody-antigen complex further continues to migrate along the strip and is captured in the detection zone by a capture antibody, resulting in a visible change of color. Several LFAs for the detection of different BoNT serotypes have been developed with detection limits usually between 0.3 and 250 ng/mL (Chiao et al 2004(Chiao et al , 2008aKlewitz et al 2006;Attrée et al 2007;Han et al 2007). Sharma and co-workers evaluated two commercial products on spiked food samples and found detection limits of above 20 ng/mL for BoNT complexes of serotypes A, B, and E (Sharma et al 2005).…”

Section: Rapid Detection Tools Based On Elisa Formatsmentioning

confidence: 99%

Complexity of Botulinum Neurotoxins: Challenges for Detection Technology

Dorner

Schulz

Kull

et al. 2012

Current Topics in Microbiology and Immunology

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The detection of botulinum neurotoxins (BoNT) is extremely challenging due to their high toxicity and the multiple BoNT variants. To date, seven serotypes with more than 30 subtypes have been described, and even more subtypes are expected to be discovered. The fact that the BoNT molecules are released as large complexes of different size and composition adds further complexity to the issue. Currently, in the diagnostics of botulism, the mouse bioassay (MBA) is still considered as gold standard for the detection of BoNT in complex sample materials. Over the years, different functional, immunological, and spectrometric assays or combinations thereof have been developed, supplemented by DNA-based assays for the detection of the organism. In this review, advantages and limitations of the current technologies will be discussed, highlighting some of the intricacies of real sample analysis.

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“…Gold nanoparticles (GNPs) have recently attracted significant attention due to their non-toxic nature and excellent biological compatibility [20,21]. Therefore, gold-labeled antibodies have provided attractive means for developing biosensors without the handling of toxic reagents [22][23][24][25]. Unlike fluorescence or enzyme-detection systems, gold-labeled antibodies are more stable and easy to use.…”

Section: Introductionmentioning

confidence: 99%

Application of gold-labeled antibody biosensor in simultaneous determination of total aflatoxins using artificial neural network

Saidi

Mirzaei

2013

J IRAN CHEM SOC

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Preparation of antibody-coated gold nanoparticles (GNPs) specific to aflatoxins B1, B2, G1 and G2 and its use in developing aflatoxins diagnostic method were presented in this paper. The formation of gold-labeled antibodies was accomplished at optimal condition. Due to severe overlapping between the emission profiles for the aflatoxins, they cannot be determined by direct inspection of data. The strategy used in this study, constituted by artificial neural network (ANN), was easy to implement and to originate reliable results. ANN can be successfully applied to spectrofluorimetric spectra matrices to simultaneous determination of total aflatoxins. Quantitative results obtained using ANN method for aflatoxins in pistachio nuts samples were compared to those obtained using the HPLC method. Obtained results using these two methods did not show significant differences.

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Colloidal gold-based immunochromatographic assay for detection of botulinum neurotoxin type B

Cited by 117 publications

References 13 publications

Expression of the capsid protein of Chikungunya virus in a baculovirus for serodiagnosis of Chikungunya disease

Expression of the capsid protein of Chikungunya virus in a baculovirus for serodiagnosis of Chikungunya disease

Complexity of Botulinum Neurotoxins: Challenges for Detection Technology

Application of gold-labeled antibody biosensor in simultaneous determination of total aflatoxins using artificial neural network

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