Colocalization pattern of calbindin and cocaine- and amphetamine-regulated transcript in the mammillary body–anterior thalamic nuclei axis of the guinea pig

Żakowski, Witold; Równiak, Maciej; Robak, Anna

doi:10.1016/j.neuroscience.2013.12.015

Cited by 12 publications

(9 citation statements)

References 55 publications

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“…Interestingly, and in stark contrast to our Nts -cre results, we observed highly specific labeling of the AV subdivision of the ATN, while sparing the AM and AD. These data are consistent with immunohistochemical evidence in guinea pig suggesting that CART (encoded by Cartpt ) and calbindin (encoded by Calb1 ) are co-localized in the ML region of the MB and calbindin-immunoreactive fibers are concentrated in the AV subdivision of the ATN ( Żakowski et al, 2014 ). Taken together, these anterograde tracing data suggest that not only are molecularly-defined subpopulations of MB neurons highly segregated within the anatomical subdivisions of the MB (MnM, MM, ML, and LM), but these subpopulations appear to project to distinct domains within the ATN, consistent with previous anterograde and retrograde tracing data without genetic specificity ( Aggleton et al, 2010 ; Shibata, 1992 ; Jankowski et al, 2013 ; Bubb et al, 2017 ; Seki and Zyo, 1984 ; Hayakawa and Zyo, 1989 ; Wright et al, 2013 ).…”

Section: Resultssupporting

confidence: 91%

Cellular taxonomy and spatial organization of the murine ventral posterior hypothalamus

Mickelsen

Flynn

Springer

et al. 2020

eLife

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The ventral posterior hypothalamus (VPH) is an anatomically complex brain region implicated in arousal, reproduction, energy balance, and memory processing. However, neuronal cell type diversity within the VPH is poorly understood, an impediment to deconstructing the roles of distinct VPH circuits in physiology and behavior. To address this question, we employed a droplet-based single-cell RNA sequencing (scRNA-seq) approach to systematically classify molecularly distinct cell populations in the mouse VPH. Analysis of >16,000 single cells revealed 20 neuronal and 18 non-neuronal cell populations, defined by suites of discriminatory markers. We validated differentially expressed genes in selected neuronal populations through fluorescence in situ hybridization (FISH). Focusing on the mammillary bodies (MB), we discovered transcriptionally-distinct clusters that exhibit neuroanatomical parcellation within MB subdivisions and topographic projections to the thalamus. This single-cell transcriptomic atlas of VPH cell types provides a resource for interrogating the circuit-level mechanisms underlying the diverse functions of VPH circuits.

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Section: Resultssupporting

confidence: 91%

Cellular taxonomy and spatial organization of the murine ventral posterior hypothalamus

Mickelsen

Flynn

Springer

et al. 2020

eLife

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“…a combination of intrinsic excitation (unit bursting) and extrinsic inhibition (parvalbumineric/GABAergic input) is thought to be one mechanism through which network theta activity in other theta-generating circuitry is generated ( Colgin, 2013 ; Hu et al, 2002 ; Hutcheon and Yarom, 2000 ; Stark et al, 2013 ). Accordingly, the medial MBs express genes promoting rhythmic firing behaviour such as T-type calcium channel receptor subunits ( Talley et al, 1999 ) as well as various calcium binding proteins, including parvalbumin, calbindin, calretinin and hippocalcin ( Bernstein et al, 2007 ; Celio, 1990 ; Fortin and Parent, 1997 ; Paterlini et al, 2000 ; Zakowski et al, 2014 ). In addition, the medial MBs are also subject to diverse neuromodulatory influences, which are likely to support oscillatory activity and, more broadly, their role in memory.…”

Section: Electrophysiological Propertiesmentioning

confidence: 99%

Time to put the mammillothalamic pathway into context

Dillingham

Milczarek

Perry

et al. 2021

Neuroscience & Biobehavioral Reviews

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“…CB and PV serve as markers of postnatal stages during development of the guinea pig anterior thalamic and septum, respectively (Zakowski et al, ; Hermanowicz‐Sobieraj and Robak, ). These proteins precisely mark also two functionally differentiated subsystems within the mammillary body‐anterior thalamic nuclei system (Zakowski et al, ). Although CB, CR, and PV were used as excellent markers of important cell populations in multiple brain regions, still, little is known about populations expressing these proteins in the preoptic area of the hypothalamus.…”

Section: Introductionmentioning

confidence: 99%

Ontogeny of Neurons Containing Calcium‐Binding Proteins in the Preoptic Area of the Guinea Pig: Sexually Dimorphic Development of Calbindin

Bogus‐Nowakowska

2019

Developmental Neurobiology

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This study characterizes for the first time the distribution and coexistence patterns of calbindin (CB), calretinin (CR), and parvalbumin (PV) in the female and male guinea pig preoptic area (POA) during brain development, using immunohistochemistry and quantitative real‐time PCR techniques. The results show that the prenatal development of the guinea pig POA takes place in elevated levels of CB and CR immunoreactivity with the peak at embryonic day 50 (E50) and generally in newborns both these proteins reach an adult‐like pattern of immunoreactivity, contrary to PV which appears later, peaks at postnatal day (PND) 10 (P10), and stabilizes at P20. CB and CR have also overlapping distributions which differed from that of PV, and much higher expressions at mRNA and protein levels. However, CB‐positive (+), CR+ and PV+ neurons create in the guinea pig POA separate populations as CB and CR coexisted only in a small number of neurons and CB+ cells never coexpressed PV. Moreover, the density of CB+ neurons, contrary to CR+ and PV+ cells, is sexually dimorphic favoring males at all the examined stages. In conclusion, elevated levels of CR and CB at the time of intense cell migration, differentiation, myelination, and synaptogenesis in the guinea pig brain suggest that these proteins may be engaged in similar processes in the POA, while late onset of PV may be rather linked with POA maturation. As the population of CB+ cells in the POA is very large, its dimorphic development may have huge impact on the sexual differentiation of this brain region.

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Colocalization pattern of calbindin and cocaine- and amphetamine-regulated transcript in the mammillary body–anterior thalamic nuclei axis of the guinea pig

Cited by 12 publications

References 55 publications

Cellular taxonomy and spatial organization of the murine ventral posterior hypothalamus

Cellular taxonomy and spatial organization of the murine ventral posterior hypothalamus

Time to put the mammillothalamic pathway into context

Ontogeny of Neurons Containing Calcium‐Binding Proteins in the Preoptic Area of the Guinea Pig: Sexually Dimorphic Development of Calbindin

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