Colonization by high-level aminoglycoside-resistant enterococci in intensive care unit patients: epidemiology and clinical relevance

Ramírez, Encarnación; Muniain, Miguel A.; Santos, Jéssica Diane dos; Joyanes, Providencia; González, F. Baigorri; García-Sánchez, M; Martínez-Martínez, Luis

doi:10.1016/j.jhin.2004.12.024

Cited by 8 publications

(5 citation statements)

References 35 publications

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“…These results are in agreement with other studies (Simonsen et al, 2003;Iris et al, 2014). However, researchers from Spain (Rodriguez-Bano et al, 2005) obtained only 28.6% ampicillin-resistant E. faecium strains, whereas in the Netherlands (Schouten et al, 1999) 24% of E. faecium strains were susceptible to imipenem. It should be noted that in this study most of the tested strains that were resistant to β-lactams were also resistant to gentamicin or streptomycin.…”

Section: Discussionsupporting

confidence: 92%

“…Moreover, this species is also able to acquire resistance by means of mutations or as a result of the transfer of genes located on plasmids, transposons, or due to the incorporation of integrons (Chen et al, 2009;Sieńko et al, 2014). In the standard treatment of enterococcal infections, the use of a cell wall active agent (β-lactam, glycopeptide) with an aminoglycoside results in synergistic bactericidal activity (Rodriguez-Bano et al, 2005). E. faecium has high-level resistance to many β-lactams as a consequence of overproduction and modification of penicillin-binding proteins (PBP), particularly PBP5 (Rodriguez-Bano et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…In the standard treatment of enterococcal infections, the use of a cell wall active agent (β-lactam, glycopeptide) with an aminoglycoside results in synergistic bactericidal activity (Rodriguez-Bano et al, 2005). E. faecium has high-level resistance to many β-lactams as a consequence of overproduction and modification of penicillin-binding proteins (PBP), particularly PBP5 (Rodriguez-Bano et al, 2005). Although rare, this resistance is mediated by the production of a β-lactamase enzyme (Rodriguez-Bano et al, 2005;Comerlato et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…E. faecium has high-level resistance to many β-lactams as a consequence of overproduction and modification of penicillin-binding proteins (PBP), particularly PBP5 (Rodriguez-Bano et al, 2005). Although rare, this resistance is mediated by the production of a β-lactamase enzyme (Rodriguez-Bano et al, 2005;Comerlato et al, 2013). Bacteria resistant to glycopeptides produce cell wall precursors with decreased affinity for the drug administered to treat an infection, which prevents the antibiotic from blocking cell wall synthesis (Sacha et al, 2008;Cheng et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of antibiotic resistance and virulence between biofilm-producing and non-producing clinical isolates of Enterococcus faecium

et al. 2015

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An increase in the antibiotic resistance among Enterococcus faecium strains has been observed worldwide. Moreover, this bacteria has the ability to produce several virulence factors and to form biofilm that plays an important role in human infections. This study was designed to compare the antibiotic resistance and the prevalence of genes encoding surface protein (esp), aggregation substance (as), surface adhesin (efaA), collagen adhesin (ace), gelatinase (gelE), and hialuronidase (hyl) between biofilm-producing and non-producing E. faecium strains. Therefore, ninety E. faecium clinical isolates were tested for biofilm-forming ability, and then were assigned to two groups: biofilm-positive (BIO(+), n =70) and biofilm-negative (BIO(-), n = 20). Comparison of these groups showed that BIO(+) isolates were resistant to β-lactams, whereas 10% of BIO(-) strains were susceptible to ampicillin (statistically significant difference, p = 0.007) and 5% to imipenem. Linezolid and tigecycline were the only antibiotics active against all tested isolates. Analysis of the virulence factors revealed that ace, efaA, and gelE genes occurred more frequently in BIO(-) strains (ace in 50% BIO(+) vs. 75% BIO(-); efaA 44.3% vs. 85%; gelE 2.9% vs. 15%, respectively), while hyl gene appeared more frequently in BIO(+) isolates (87.1% BIO(+) vs. 65% BIO(-)). These differences were significant (p < 0.05). We concluded that BIO(+) strains were more resistant to antibiotics than BIO(-) strains, but interestingly, BIO(-) isolates were characterized by possession of higher virulence capabilities.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comparison of antibiotic resistance and virulence between biofilm-producing and non-producing clinical isolates of Enterococcus faecium

et al. 2015

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“…Enterococcus faecalis is a very important bacterial specie as a pathogen acquired in hospitals of USA and Europe, especially in intensive care units, where are one of the main causes of bacteremia (Rodríguez-Baño et al 2005). Although there are various surveys addressed to know the causes because these microorganisms induce human infections, diverse aspects of its biology have not been revealed (Fisher and Phillips 2009).…”

Section: Discussionmentioning

confidence: 99%

Virulence, bacterocin genes and antibacterial susceptibility in Enterococcus faecalis strains isolated from water wells for human consumption

Padilla

Lobos

2013

SpringerPlus

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The objectives of this study were to detect genes for virulence and bacteriocins in addition to studying the antimicrobial susceptibility of 78 strains of E. faecalis isolated from water wells for human consumption. The virulence and bacteriocin genes of 78 E. faecalis were amplified by PCR and visualized in agarose gels. The antimicrobial susceptibility was determined through diffusion agar tests and the MIC through microdilution. It was observed that the major percentage of virulence genes in the E. faecalis strains corresponds to aggA (93.5%). The bacteriocin gene entA (64.1%) is the most frequently detected. The studied strains exhibited different virulence and bacteriocin genes, and an important antibacterial resistance. The most common resistant phenotype (n = 14) corresponds to tetracycline and chloramphenicol and the less frequent (n = 2) to ciprofloxacin and moxifloxacin. Eight different genetic profiles were observed for virulence y bacteriocin genes. It was determined a statistical association between the bacterial resistance and some of the genetic profiles detected.

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Occurrence of aminoglycoside-modifying-enzyme genesaac(6′)-aph(2″), aph(3′), ant(4′) andant(6) in clinical isolates ofEnterococcus faecalis resistant to high-level of gentamicin and amikacin

et al. 2006

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The genes coding for 4 aminoglycoside-modifying enzymes AAC(6')-APH(2"), APH(3'), ANT(4') and ANT(6) were determined in 44 Slovak clinical isolates of Enterococcus faecalis with high-level resistance to gentamicin (HLGR, collection 1) and 48 E. faecalis isolates with resistance to amikacin (AR, collection 2). The occurrence of spotted genes was (collection 1 vs. collection 2): aac(6)-aph(2") 81.8 vs. 8.3 %, ant(4') 52.3 vs. 81.3 %, aph(3') 50 vs. 56.3 % and ant(6) 6.8 vs. 4.2 %, the most frequent combinations of genes in the HLGR collection were aac(6')-aph(2") + ant(4') and aac(6')-aph(2") + aph(3). In contrast, the aph(3') + ant(4') gene profile was predominant in AR isolates. None of the isolates contained all four AGME genes simultaneously.

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Colonization by high-level aminoglycoside-resistant enterococci in intensive care unit patients: epidemiology and clinical relevance

Cited by 8 publications

References 35 publications

Comparison of antibiotic resistance and virulence between biofilm-producing and non-producing clinical isolates of Enterococcus faecium

Comparison of antibiotic resistance and virulence between biofilm-producing and non-producing clinical isolates of Enterococcus faecium

Virulence, bacterocin genes and antibacterial susceptibility in Enterococcus faecalis strains isolated from water wells for human consumption

Occurrence of aminoglycoside-modifying-enzyme genesaac(6′)-aph(2″), aph(3′), ant(4′) andant(6) in clinical isolates ofEnterococcus faecalis resistant to high-level of gentamicin and amikacin

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