Colonization of Beauveria bassiana (Balsamo) Vuillemin strains in maize (Zea mays L.) and their efficacy against stem borer Chilo partellus (Swinhoe)

Abstract

ABSTRACT:Glasshouse experiments were conducted to study the endophytic ability of six strains of entomopathogenic fungi Beauveria bassiana in maize leaf and stem tissues when applied through foliar sprays of oil formulations. B. bassiana treated and untreated control plants were artificially infested with second instar larvae of Chilo partellus to assess the stem borer damage. The variation in per cent colonization and persistence was observed in all the six strains in stem and leaf tissues of maize. The mean per cent colonization was observed in older stem tissues (16.67-46.67 %), in young stem tissues (22.22-52.22 %), in older leaf tissues (25.56-47.78 %) and in young leaf tissues (31.11-57.78 %). Evaluation of six B. bassiana strains, revealed that the isolate Bb-45 recorded the highest mean colonization in mature stem (46.67 %), mature leaf (47.78%) and also in relatively younger stem (52.22 %). The Bb-5a isolate also exhibited highest mean colonization in young leaf tissues (57.78 %). Lower percentage of dead hearts (2.2-11.1%) and lower stem tunneling (2.7-4.3cm/ plant) was observed significantly in B. bassiana treated plants as compared to the untreated control plants (28.86% of dead hearts and 13.41cm/plant stem tunneling).

Solid State Fermentation of Orange Peels for Production of Cellulase, Pectinase and Recovery of Orange Oil using Aspergillus Species NCIM 1432.

“…The surface sanitisation of orange peels using 70 % v/v ethanol was found to be more appropriate as it prevented the contamination, oil evaporation, and the drying of the peels as seen in case of autoclaving and UV-light methods. Gurulingappa et al [35], have reported the surface sterilisation using 70 % ethanol in tissue culture laboratories as an effective method which is in sync with the ndings of the present research.…”

Endophytic colonization of tomato plants by Beauveria bassiana Vuillemin (Ascomycota: Hypocreales) and leaf damage in Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) larvae

“…This difference could be attributed to the compatibility of Bb 115 genome which may be more compatible with the internal environment of the plant. Several factors such as the origin, the nature of the entomopathogen, the penetration site, the inoculation method, and the compatibility with the host plant can also influence the endophytic capacity of a fungal isolate compared to another isolate (Renuka et al 2017). However, the lower rate of endophytic colonization of B. bassiana observed at the isolate Bb 11 at the concentration of 1 × 10 7 conidia/ml could be explained by a limited penetration of germinating conidia in tomato plants.…”

Performance of Metarhizium rileyi Nm017: nutritional supplementation to improve production and quality conidia

“…g. , M. rileyi strains growth on a medium rich in soybeans (1.5 × 10 9 conidia g −1 substrate; Caro et al 2005 ), wheat bran enriched with malt soda (3.0 × 10 9 conidia g −1 substrate; Villamizar et al 2004 ), and crushed sorghum (1.4 × 10 9 conidia g −1 substrate; Devi et al 2001 ). Also, Nm017 production presented appropriate biomass yields for commercial entomopathogenic species, like M. anisopliae (27–42.2 g kg −1 ) and B. bassiana (20–40 g kg −1 ) (Keppanan et al 2018 ; Tumuhaise et al 2018 ; Liu et al 2015 ; Pham et al 2010 ; Posada-Flórez 2008 ; Renuka et al 2015 ). This could be due to the substrate used in this study (rice), in which most fungi reach the highest biomass production (Gouli et al 2013 ; Li et al 2010 ; Jaihan et al 2016 ; Saldarriaga et al 2017 ).…”